ALOX15在慢性鼻窦炎伴鼻息肉中表达及糖皮质激素对其作用研究

doi:10.3969/j.issn.1006-7795.2017.05.011

首都医科大学学报 ›› 2017, Vol. 38 ›› Issue (5): 683-687.doi: 10.3969/j.issn.1006-7795.2017.05.011

ALOX15在慢性鼻窦炎伴鼻息肉中表达及糖皮质激素对其作用研究

闫冰¹, 王阳¹, 范尔钟¹, 李颖¹, 齐思涵², 楚晓晗², 王成硕², 张罗^1,2

1. 北京市耳鼻咽喉科研究所北京市鼻病重点实验室教育部耳鼻咽喉头颈外科重点实验室, 北京 100005;
2. 首都医科大学附属北京同仁医院耳鼻咽喉头颈外科, 北京 100730

收稿日期:2017-07-07 出版日期:2017-09-21 发布日期:2017-10-18
通讯作者: 王成硕, 张罗 E-mail:wangcs830@126.com;dr.luozhang@139.com
基金资助:
国家自然科学基金重点项目（81630023，81400444，81470678，81420108009），北京市医院管理局"使命"计划（SML20150203），北京市医院管理局"青苗"计划（QML20150202），首都临床特色应用研究（Z141107002514122），首都卫生发展科研专项重点攻关项目（2016-1-2052），北京市"百千万"人才项目（2016）。

Expression and corticosteroid inhibition of arachidonate 15-lipoxygenase in chronic rhinosinusitis with nasal polyps

Yan Bing¹, Wang Yang¹, Fan Erzhong¹, Li Ying¹, Qi Sihan², Chu Xiaohan², Wang Chengshuo², Zhang Luo^1,2

1. Beijing Institute of Otorhinolaryngology, Beijing Key Laboratory of Nasal Diseases, Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education of China, Beijing 100005, China;
2. Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China

Received:2017-07-07 Online:2017-09-21 Published:2017-10-18
Supported by:
This study was supported by National Natural Science Foundation of China (81630023, 81400444, 81470678, 81420108009), Beijing Municipal Administration of Hospitals' Mission Plan (SML20150203), Beijing Municipal Administration of Hospitals' Youth Programme (QML20150202), Beijing Clinical Research Foundation (Z141107002514122), Capital's Funds for Health Improvement and Research (2016-1-2052), Talents Project of Beijing Municipal Human Resources and Social Security Bureau (2016).

摘要/Abstract

摘要： 目的研究花生四烯酸酯15-脂氧合酶（arachidonate 15-lipoxygenase，ALOX15）在嗜酸粒细胞型慢性鼻窦炎伴鼻息肉（eosinophilic chronic rhinosinusitis with nasal polyps，ECRSwNP）及非嗜酸粒细胞型慢性鼻窦炎伴鼻息肉（nonesinophilc chronic rhinosinusitis with nasal polyps，nonECRSwNP）中表达，并研究口服糖皮质激素对其表达的影响。方法所有标本均由首都医科大学附属北京同仁医院标本库中获得，采用real-time PCR及免疫组织化学染色研究ALOX15表达与蛋白定位。比较激素治疗前ECRSwNP、nonECRSwNP及健康对照组中ALOX15 mRNA表达水平、蛋白定位及表达，并比较同一病人激素治疗前后mRNA及蛋白表达分布差异。结果 ECRSwNP组中ALOX15 mRNA及蛋白表达量高于健康对照组及nonECRSwNP组，差异有统计学意义（均P=0.000）。健康对照组、nonECRSwNP组间差异无统计学意义（P=0.594，P=0.550）。ALOX15蛋白表达于上皮细胞、浆细胞、中性粒细胞及嗜酸性粒细胞等多种细胞。ECRSwNP组激素治疗前后ALOX15 mRNA和蛋白表达差异有统计学意义（P=0.004，P=0.024）。结论 ALOX15在ECRSwNP中表达显著高于nonECRSwNP及健康对照组，口服糖皮质激素1周能抑制其表达。

关键词: 鼻息肉, 慢性鼻窦炎, 花生四烯酸酯15-脂氧合酶

Abstract: Objective To detect the expression of arachidonate 15-lipoxygenase (ALOX15) in eosinophilic chronic rhinosinusitis with nasal polyps (ECRSwNP), and nonesinophilc chronic rhinosinusitis with nasal polyps (nonECRSwNP). Moreover, the effect of corticosteroid on ALOX15 expression was evaluated. Methods Samples were socked in RNA later and embedded in paraffin were acquired from library of Beijing Tongren Hospital. Real-time PCR and immunohistochemistry (IHC) were applied for mRNA quantification and protein distribution of ALOX15 in ECRSwNP, nonECRSwNP and healthy control group respectively.ALOX15 mRNA level of ECRSwNP samples pre-and post-corticosteroid treatment were detected. Results LOX15 mRNA level and protein expression were found increased significantly in ECRSwNP compared with nonECRSwNP and healthy control (both P=0.000). There was no significant difference between nonECRSwNP and healthy control (P=0.594, P=0.550). ALOX15 protein localized in a majority of cells including epithelia, plasma cells,neutrophils and eosinophils.One-week oral corticosteroid could decrease ALOX15 mRNA and protein expression in ECRSwNP significantly (P=0.004, P=0.024). Conclusion ALOX15 is upregulated in ECRSwNP compared with nonECRSwNP and control groups. Corticosteroid treatment could inhibit ALOX15 expression in ECRSwNP.

Key words: nasal polyps, chronic rhinosinusitis, arachidonate 15-lipoxygenase

中图分类号:

R765.4

闫冰, 王阳, 范尔钟, 李颖, 齐思涵, 楚晓晗, 王成硕, 张罗. ALOX15在慢性鼻窦炎伴鼻息肉中表达及糖皮质激素对其作用研究[J]. 首都医科大学学报, 2017, 38(5): 683-687.

Yan Bing, Wang Yang, Fan Erzhong, Li Ying, Qi Sihan, Chu Xiaohan, Wang Chengshuo, Zhang Luo. Expression and corticosteroid inhibition of arachidonate 15-lipoxygenase in chronic rhinosinusitis with nasal polyps[J]. Journal of Capital Medical University, 2017, 38(5): 683-687.

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ALOX15在慢性鼻窦炎伴鼻息肉中表达及糖皮质激素对其作用研究

Expression and corticosteroid inhibition of arachidonate 15-lipoxygenase in chronic rhinosinusitis with nasal polyps

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