细胞连接在慢性鼻窦炎中的表达及意义

doi:10.3969/j.issn.1006-7795.2013.06.004

首都医科大学学报 ›› 2013, Vol. 34 ›› Issue (6): 799-804.doi: 10.3969/j.issn.1006-7795.2013.06.004

• 过敏性鼻炎和慢性鼻窦炎 • 上一篇下一篇

细胞连接在慢性鼻窦炎中的表达及意义

李颖^1,2, 范尔钟^1,2, 伯铭羽¹, 王向东^1,2, 张罗^1,2

1. 首都医科大学附属北京同仁医院耳鼻咽喉头颈外科, 北京 100005;
2. 北京市耳鼻咽喉科研究所, 耳鼻咽喉头颈科学教育部重点实验室, 鼻病研究北京市重点实验室, 北京 100005

收稿日期:2013-10-08 出版日期:2013-12-21 发布日期:2013-12-13
通讯作者: 张罗 E-mail:dr.luozhang@gmail.com
基金资助:
国家杰出青年科学基金（81025007）。

Expression of cell junctions in the tissue of chronic rhinosinusitis

Li Ying^1,2, Fan Erzhong^1,2, Bo Mingyu¹, Wang Xiangdong^1,2, Zhang Luo^1,2

1. Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, China;
2. Beijing Institute of Otorhinolaryngology, Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Beijing Key Laboratory of Nasal Diseases, Beijing 100005, China

Received:2013-10-08 Online:2013-12-21 Published:2013-12-13
Supported by:
This study was supported by the National Science Foundation for Distinguished Young Scholars(81025007).

摘要/Abstract

摘要：

目的探讨3种细胞连接蛋白在不同类型慢性鼻窦炎中的表达情况及其对上皮屏障功能改变的意义。方法共66例患者，其中慢性鼻窦炎伴息肉者30例，分为嗜酸性粒细胞性鼻息肉组（15例）、非嗜酸性粒细胞性鼻息肉组（15例），慢性鼻窦炎不伴息肉组15例，正常对照组为鼻中隔偏曲患者共15例。用免疫组织化学染色检测各组中紧密连接蛋白（zonular occludens-1，ZO-1）、黏附连接蛋白（E-cadherin）和桥粒连接蛋白（desmoglein-1，dsg-1）的表达情况。结果 ZO-1的表达中，嗜酸性粒细胞性鼻息肉组与正常对照组相比有明显下降（P<0.05）。E-cadherin的表达中，鼻息肉组和慢性鼻窦炎不伴息肉组与对照组相比呈明显上调表达（P<0.05），而dsg-1呈明显下调表达（P<0.05），并且鼻息肉组dsg-1的表达与慢性鼻-鼻窦炎不伴息肉组相比也呈明显下调表达（P<0.05）。结论 3种不同类型的细胞连接蛋白在慢性鼻-鼻窦炎中的表达均有不同程度的改变，说明连接蛋白表达的改变与上皮屏障功能的破坏可能在慢性鼻窦炎的发病机制中发挥重要的作用。

关键词: 慢性鼻窦炎, 细胞连接蛋白, 上皮屏障功能

Abstract:

Objective To evaluate the expression of cell junctions in the tissue of chronic rhinosinusitis and their roles in barrier function of epithelium. Methods Eosinophilic nasal polyps and non-eosinophilic nasal polyps of chronic rhinosinusitis with nasal polyps(CRSwNP), sinus mucosa of chronic rhinosinusitis without nasal polyps(CRSsNP) and control mucosa were enrolled in this study. Each group included 15 cases. Zonulae occludens(ZO-1), E-cadherin and desmoglein-1(dsg-1) were stained by immunohistochemistry. Results The expression of ZO-1 in eosinophilic nasal polyps group were significantly less than that in other groups(P<0.05). The expressions of E-cadherin in nasal polyps and CRSsNP group were significantly more than that in control group(P<0.05). The expressions of dsg-1 in nasal polyps groups and CRSsNP group were significantly less than that in control group(P<0.05), while dsg-1 of nasal polyps groups were significantly less than that of CRSsNP group(P<0.05). Conclusion The changes of cell junctions may play important roles in the pathogenesis of chronic rhinosinusitis.

Key words: chronic rhinosinusitis, cell junction, epithelial barrier function

中图分类号:

R765.4

李颖, 范尔钟, 伯铭羽, 王向东, 张罗. 细胞连接在慢性鼻窦炎中的表达及意义[J]. 首都医科大学学报, 2013, 34(6): 799-804.

Li Ying, Fan Erzhong, Bo Mingyu, Wang Xiangdong, Zhang Luo. Expression of cell junctions in the tissue of chronic rhinosinusitis[J]. Journal of Capital Medical University, 2013, 34(6): 799-804.

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细胞连接在慢性鼻窦炎中的表达及意义

Expression of cell junctions in the tissue of chronic rhinosinusitis

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