人类白细胞抗原Ⅱ类基因多态性与中国人群尘螨过敏性鼻炎相关性研究

doi:10.3969/j.issn.1006-7795.2017.05.012

首都医科大学学报 ›› 2017, Vol. 38 ›› Issue (5): 688-694.doi: 10.3969/j.issn.1006-7795.2017.05.012

人类白细胞抗原Ⅱ类基因多态性与中国人群尘螨过敏性鼻炎相关性研究

赵延明¹, 王成硕¹, 赵亚丽², 张媛^2,3, 张罗^1,2,3

1. 首都医科大学附属北京同仁医院耳鼻咽喉头颈外科, 北京 100730;
2. 北京市耳鼻咽喉科研究所鼻病研究北京市重点实验室, 北京 100005;
3. 首都医科大学附属北京同仁医院变态反应科, 北京 100730

收稿日期:2017-07-07 出版日期:2017-09-21 发布日期:2017-10-18
通讯作者: 张媛, 张罗 E-mail:summer_zhang1211@126.com;dr.luozhang@139.com
基金资助:
国家自然科学基金（81420108009，81630023，81470678，30872846），北京市自然科学基金（7102030），北京市卫生局青年科学研究资助课题（QN2008-024）。

Association between human leukocyte antigenⅡ (HLA-Ⅱ) gene polymorphism and house dust mites-sensitive allergic rhinitis in Chinese subjects

Zhao Yanming¹, Wang Chengshuo¹, Zhao Yali², Zhang Yuan^2,3, Zhang Luo^1,2,3

1. Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China;
2. Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otorhinolaryngology, Beijing 100005, China;
3. Department of Allergy, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China

Received:2017-07-07 Online:2017-09-21 Published:2017-10-18
Supported by:
This study was supported by National Natural Science Foundation of China(81420108009,81630023,81470678,30872846),Natural Science Foundation of Beijing(7102030),Funds for Young Scholars by Beijing Public Health Bureau(QN2008-024)

摘要/Abstract

摘要： 目的探索人类白细胞抗原Ⅱ类基因（human leukocyte antigenⅡ，HLA-Ⅱ）多态性在中国人群中尘螨过敏性鼻炎发病中的作用。方法选取单纯尘螨过敏性鼻炎（allergic rhinitis，AR）病人71例，健康对照92例，提取受试者外周血DNA，应用聚合酶链反应序列分型法（polymerase chain reaction sequence-based genotyping，PCR-SBT）进行HLA-Ⅱ 基因（DRB1、DQB1）分型。采用R软件进行HLA-Ⅱ基因频率统计及单倍体型分析包进行统计学分析，基因频率在AR与对照组中的比较应用χ²及Fisher精确检验分析，结果进行Bonferroni多重矫正。结果共检测到16个HLA-DRB1等位基因，13个HLA-DQB1等位基因。其中DQB1*06：01：01（P_校正=0.010，OR=2.347，95%CI：1.392~4.225）、DRB1*08：03：02（P_校正=0.012，OR=3.213，95%CI：1.732~7.821），在尘螨过敏性病人中的基因频率较健康对照组增加，差异具有统计学意义。结果提示，HLA-DRB1*08：03：02和HLA-DQB1*06：01：01等位基因可能是中国人群尘螨过敏性鼻炎发病的风险因子。单倍体型DRB1*08：03-DQB1*06：01（19%vs 4.8%，P_校正=0.007，OR=4.232，95%CI：1.822~9.237）在病例组中频率增加。结论 HLA-DRB1*08：03：02和HLA-DQB1*06：01：01等位基因可能是中国人群尘螨过敏性鼻炎发病的风险因子。

关键词: 过敏性鼻炎, 尘螨, 人类白细胞抗原, DQB1, DRB1, 基因多态性

Abstract: Objective To elucidate the human leukocyte antigenⅡ (HLA-Ⅱ) gene alleles associated with allergic rhinitis (AR) in house dust mites sensitive (HDM-sensitive) Han Chinese subjects. Methods Seventy-one patients with AR and 92 healthy subjects were recruited to this case control study. Frequencies of HLA were estimated by using the R statistical analysis package. Significance was evaluated using χ² test and Fisher exact test. For multiple allelic frequency comparisons, Bonferroni correction was applied. Genotyping of HLA-DRB1 and HAL-DQB1 was performed by the polymerase chain reaction sequence-based genotyping method. Results A total of 16 alleles of the HLA-DRB1 locus and 13 alleles of the HLA-DQB1 locus were genotyped in the entire study cohort; of which the frequencies of alleles DQB1*06:01:01 (P=0.010, OR=2.347, 95% CI:1.392-4.225) and DRB1*08:03:02 (P=0.012, OR=3.213, 95% CI:1.732-7.821) were significantly increased in HDM-sensitive AR patients compared to healthy controls and considered to be a risk factor for AR in Chinese Han subjects. The frequency of haplotype DRB1*08:03-DQB1*06:01 in HDM-sensitive AR group was also significantly higher than that in the control group (19% vs 4.8%, P=0.007, OR=4.232;95%CI:1.822-9.237). Conclusion HLA-DRB1*08:03:02 and HLA-DQB1*06:01:01 are associated with mites-sensitive AR and may confer a risk for development of AR in Han Chinese subjects sensitized to HDM.

Key words: allergic rhinitis, house dust mites(HDMs), human leukocyte antigen(HLA), DQB1, DRB1, gene polymorphism

中图分类号:

R765

赵延明, 王成硕, 赵亚丽, 张媛, 张罗. 人类白细胞抗原Ⅱ类基因多态性与中国人群尘螨过敏性鼻炎相关性研究[J]. 首都医科大学学报, 2017, 38(5): 688-694.

Zhao Yanming, Wang Chengshuo, Zhao Yali, Zhang Yuan, Zhang Luo. Association between human leukocyte antigenⅡ (HLA-Ⅱ) gene polymorphism and house dust mites-sensitive allergic rhinitis in Chinese subjects[J]. Journal of Capital Medical University, 2017, 38(5): 688-694.

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人类白细胞抗原Ⅱ类基因多态性与中国人群尘螨过敏性鼻炎相关性研究

Association between human leukocyte antigenⅡ (HLA-Ⅱ) gene polymorphism and house dust mites-sensitive allergic rhinitis in Chinese subjects

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