大鼠非心肌细胞对心肌细胞凋亡的影响

首都医科大学学报 ›› 2007, Vol. 28 ›› Issue (4): 430-433.

大鼠非心肌细胞对心肌细胞凋亡的影响

谢苗荣, 沈潞华

首都医科大学附属北京友谊医院急诊科

收稿日期:2007-06-12 修回日期:1900-01-01 出版日期:2007-08-24 发布日期:2007-08-24

The Impact of Nonmyocytes Conditioned Media on the Biological Process of Apoptosis in Myocytes

Xie Miaorong, Shen Luhua

Departmen of Emergency Medicine, Beijing Friendship Hospital, Capital Medical University

Received:2007-06-12 Revised:1900-01-01 Online:2007-08-24 Published:2007-08-24

摘要/Abstract

摘要：

目的探讨大鼠心脏非心肌细胞对心肌细胞凋亡的影响。方法分别培养SD乳鼠心肌细胞(MC)和非心肌细胞(NMC)。制备非心肌细胞条件培养液(NMCM)。培养细胞的分组及药物干预:M组:MC+新鲜培养液;C组:MC+70%新鲜培养液+30%NMCM;L组:MC+新鲜培养液+Losartan;CL组:MC+70%新鲜培养液+30%NMCM+Losartan;P组:MC+新鲜培养液+PD123319;CP组:MC+70%新鲜培养液+30%NMCM+PD123319;LP组:MC+新鲜培养液+Losartan+PD123319;CLP组:MC+70%新鲜培养液+30%NMCM+Losartan+PD123319。用图像分析仪测定各组心肌细胞的面积,用流式细胞仪检测各组心肌细胞凋亡情况。结果用药物干预后,L组、P组及LP组与M组比较,心肌细胞面积无明显变化(P>0.05);C组及CP组细胞面积较M组、L组、P组、LP组明显增大(P<0.01)。C组及CP组细胞凋亡率明显高于其他组(P<0.001),CP组细胞凋亡较C组更为明显(P<0.05)。结论非心肌细胞培养液在促进心肌细胞肥大的同时,也促进了细胞的凋亡。Losartan可抵消非心肌细胞条件培养液促进心肌细胞凋亡的作用,提示AT₁受体介导促进心肌细胞凋亡。

关键词: 心肌细胞, 非心肌细胞, 凋亡

Abstract:

Objective To study the impact of nonmyocytes conditioned media(NMCM) on the biological process of apoptosis in myocytes(MC) and the relationship with the angiotensin Ⅱ receptors.Methods 1～3 day-old SD rats were used for our experiment.The single cells were separated to myocytes(MC) and nonmyocytes(NMC) and the suspension of NMC was used as nonmyocyte conditioned media(NMCM).After 96 hours culture,the myocytes were divided into 8 groups: group M: MC+new media;group C: MC+70% new media+30% NMCM;group L: MC+new media +Losartan(10^-6mol/L);group CL: MC+70% new media +30% NMCM+Losartan;group P: MC+ new media+ PD123319(10^-6mol/L);group CP: MC+ 70% new media +30% NMCM +PD123319;group LP: MC+new media+Losartan+PD123319;group CLP: MC+ 70% new media+30%NMCM+ Losartan+ PD123319.Results 1)The area of myocytes,RNA and protien of group C,CP and CLP were greater than those of group M;2)The rates of apoptosis of group C and CP were higher than those of other groups.Conclusion NMCM made myocytes of rats hypertrophy,but not proliferation.Losartan can inhibit both NMCM-induced hypertrophy of myocytes partly and NMCM-induced apoptosis of myocytes.It is suggest that AT1 receptor can promote apoptosis of myocytes.

Key words: myocyte, nonmyocyte, apoptosis

中图分类号:

R331.3⁺1

谢苗荣;沈潞华. 大鼠非心肌细胞对心肌细胞凋亡的影响[J]. 首都医科大学学报, 2007, 28(4): 430-433.

Xie Miaorong;Shen Luhua. The Impact of Nonmyocytes Conditioned Media on the Biological Process of Apoptosis in Myocytes[J]. Journal of Capital Medical University, 2007, 28(4): 430-433.

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