首都医科大学学报 ›› 2010, Vol. 31 ›› Issue (1): 79-83.

• 老年病的基础与临床研究 • 上一篇    下一篇

新西兰家兔大脑中动脉闭塞脑缺血模型建立的经验

闫峰1,2, 吉训明1,2, 宣芸1,2, 田欣3, 张陈诚1,2, 罗玉敏1,2
   

  1. 1. 首都医科大学宣武医院脑血管病实验室; 2. 首都医科大学脑血管病研究所;3. 首都医科大学宣武医院动物室
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-02-21 发布日期:2010-02-21
  • 通讯作者: 罗玉敏

Model of Middle Cerebral Artery Occlusion in New Zealand Rabbits

YAN Feng1,2, JI Xun-ming1,2, XUAN Yun1,2, TIAN Xin3, ZHANG Chen-cheng1,2, LUO Yu-min1,2   

  1. 1. Cerebrovascular Diseases Research Laboratory, Xuanwu Hospital, Capital Medical University; 2. Cerebrovascular Diseases Research Institute, Capital Medical University; 3. Animal Experiment Laboratory, Xuanwu Hospital, Capital Medical University
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-02-21 Published:2010-02-21
  • Contact: LUO Yu-min

PDF

337

被引次数

14

摘要：

目的 在大鼠线栓法大脑中动脉闭塞（middle cerebral artery occlusion，MCAO）模型的制作基础上，应用神经介入“微导丝导管”技术，建立一种改良的以家兔为实验对象的MCAO模型，为脑缺血研究提供新的模型。方法 分离麻醉固定后的家兔颈部动脉，在用PE-50管特制的中空导管内插入合适的导丝经颈外动脉残端剪口再经颈内动脉插入颅内，堵塞大脑中动脉。24 h后进行神经功能评分。处死家兔后用TTC染色测定脑梗死体积。结果 手术未影响家兔血气pH值、PO2、PCO2、平均动脉压（mean arterial blood pressure，MABP）及血糖水平；动物模型缺血后脑血流平均下降至插栓前的45.8%±16.2%；大脑中动脉缺血后24 h家兔行为学差异较大。脑组织TTC染色均匀，出现梗死的脑组织相对梗死体积百分比为9.43%±5.37%；动物模型成功率为54.3%。死亡原因为蛛网膜下腔出血。失败原因多为脑血管痉挛、脑血管解剖结构变异导致线栓位置有误。结论 此模型的成功率为54.3%，用于脑缺血研究时应监测脑血流以及注意术中细小的操作环节。

关键词: 家兔, 脑缺血, 模型

Abstract:

Objective To establish a modified middle cerebral artery occlusion(MCAO) model in the rabbit through a novel hollow filament based on the intraluminal suture method, to provide a new modal for studying the cerebrovascular disease. Methods The rabbit was anesthetized and fixed and the distal common carotid artery(CCA), internal carotid artery(ICA), and external carotid artery(ECA) of the rabbit were exposed. A modified PE-50 catheter(a novel hollow filament) had been introduced into the ICA through the incision of ECA and the filament was lodged in the narrow proximal anterior cerebral artery(ACA) and blocked the MCA at its origin. The neurological functional scores were evaluated at 24 h after the operation. Then the rabbits were sacrificed and 2, 3, 5-triphenyltetrazolium chloride(TTC) staining was used to determine the infarct volume. Results The pH values, PO2, PCO2, mean arterial blood pressure(MABP), and glucose were not affected by the operation. The cerebral blood flow(CBF) decreased by 45.8%±16.2 after ischemia. The neurological functional scores varied in each rabbit. The percentage of the infarct volume was 9.43%±5.37%. The rate of success was 54.3% for the model. Most rabbits died of the subarachnoid hemorrhage. Conclusion The model can be used for the study of cerebral ischemia by monitoring the cerebral blood flow and careful operation although the ratio of success was not very high.

Key words: rabbit, cerebral ischemia, model

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