关键词: 人类免疫缺陷病毒, CCR5, 急性感染期, 慢性期, 表达

Abstract: Objective To study the expression levels of human peripheral blood CD4⁺T cell surface chemokine receptor 5(CCR5),and study the difference among healthy people,HIV patients in chronic phase and HIV patients in acute phase of the expression levels of CCR5,to clarify the differences of CCR5 expression levels between HIV patients and the normal individuals,and to show the relationship of CCR5 expression levels and the progression of HIV disease,and also to clarify the significance of CCR5 expression levels in peripheral blood CD4⁺T lymphocytes of HIV patients.Methods Blood specimens were collected from 260 cases with HIV infection and 60 healthy persons from Beijing Youan Hospital,Capital Medical University.Of the 260 HIV infected cases,98 had acute HIV infection,162 had chronic HIV infection.The peripheral blood mononuclear cells(PBMC) surface CD4 and CCR5 molecules were stained by direct fluorescent monoclonal antibody,and flow cytometry was used to analyze the expression levels of CD4⁺T cell surface CCR5.The results of the flow cytometry test were analyzed by using two sample t-test.Results (1) The peripheral blood CD4⁺T lymphocyte surface CCR5 expression levels of HIV patients in acute phase and chronic phase were significantly higher than those of normal controls(P<0.01);(2) The expression level of peripheral blood CD4⁺T lymphocytes surface CCR5 of the HIV patients in chronic phase was higher than that in HIV patients in acute phase(P<0.01).Conclusion Expression levels of peripheral blood CD4⁺T lymphocytes surface CCR5 may be closely related to the susceptibility to HIV infection and the disease progression.Determination of peripheral blood CD4⁺T cell surface CCR5 expression levels may have certain important clinical significance in early diagnosis,treatment and prognosis of HIV infected patients.

Key words: human immunodeficiency virus, CD4⁺T cell surface chemokine receptor 5(CCR5), acute infection, chronic phase, expression