HIV-1慢性感染者外周血中CD8+干细胞样记忆型T细胞变化以及对疾病进展的影响

doi:10.3969/j.issn.1006-7795.2017.05.004

首都医科大学学报 ›› 2017, Vol. 38 ›› Issue (5): 650-653.doi: 10.3969/j.issn.1006-7795.2017.05.004

• 皮肤病性病诊疗与研究 • 上一篇下一篇

HIV-1慢性感染者外周血中CD8⁺干细胞样记忆型T细胞变化以及对疾病进展的影响

宋冰冰¹, 陆小凡², 翁文佳¹, 粟斌², 张彤², 高艳青¹

1. 首都医科大学附属北京佑安医院皮肤性病科, 北京 100069;
2. 北京市艾滋病研究北京市重点实验室, 北京 100069

收稿日期:2017-05-09 出版日期:2017-09-21 发布日期:2017-10-18
通讯作者: 高艳青 E-mail:gyqing2001bj@sina.com
基金资助:
北京市科技计划（D141100000314005）。

Dynamic changes of CD8⁺ stem memory T cells and their effects on disease progression in chronic HIV-1 infection

Song Bingbing¹, Lu Xiaofan², Weng Wenjia¹, Su Bin², Zhang Tong², Gao Yanqing¹

1. Department of Dermatology, Beijing Youan Hospital, Capital Medical University, Beijing 100069, China;
2. Beijing Key Laboratory of AIDS Research, Beijing 100069, China

Received:2017-05-09 Online:2017-09-21 Published:2017-10-18
Supported by:
This study was supported by Science and Technology Plan of Beijing (D141100000314005).

摘要/Abstract

摘要： 目的观察人类免疫缺陷病毒-1（human immunodeficiency virus-1，HIV-1）慢性感染者外周血中CD8⁺干细胞样记忆型T细胞（CD8⁺ stem memory T cells，CD8⁺TSCM）随治疗的动态变化，分析其与疾病进展的关系。方法观察25例经抗反转录病毒治疗（antiretroviral therapy，ART）的慢性HIV-1感染者（治疗方案为替诺福韦+依非韦伦+拉米夫定）以及36例健康对照者，对样本外周血单个核细胞（peripheral blood mononuclear cells，PBMC）分别用CD3-APCCy7、CD4-FITC、CD8-PerCPCy5、CD45RA-PECy7、CCR7-APC、CD27-PE、CD95-Pacific Blue抗体和CD38-PE、HLA DR-APC抗体进行细胞表面染色并通过流式细胞仪检测CD8⁺TSCM细胞比例。比较HIV-1慢性感染者和健康者CD8⁺TSCM细胞比例的差异，并分析其与疾病进展指标（CD4⁺T细胞计数、HIV病毒载量以及免疫活化指标）的关系。结果 HIV-1慢性感染组的CD8⁺TSCM细胞比例小于健康对照组；CD8⁺ TSCM细胞比例与CD4⁺ T细胞计数呈正相关，与HIV-1病毒载量以及免疫活化指标CD38⁺ HLA-DR⁺ CD8⁺ T细胞呈负相关；随着ART治疗，CD8⁺TSCM细胞比例在144周后升至健康对照水平。结论 CD8⁺TSCM在慢性HIV-1感染中发挥一定的保护作用，HIV-1相关特异性CD8⁺TSCM细胞可能是未来T细胞疫苗设计的靶点。

关键词: 人类免疫缺陷病毒, 干细胞样记忆性T细胞, 疾病进展

Abstract: Objective To study the dynamics of CD8⁺ stem memory T cells (TSCM) and the impact of CD8⁺ TSCM cells on disease progression of human immunodeficiency virus-1 (HIV-1) infection. Methods Twenty-five cases with chronic HIV-1 infection receiving antiretroviral therapy (ART) with tenofovir plus efavirenz + lamivudine and 36 healthy controls were enrolled and observed. Peripheral blood mononuclear cells were stained with using CD3-APCCy7, CD4-FITC, CD8-PerCPCy5, CD45RA-PECy7, CCR7-APC, CD27-PE, CD95-Pacific Blue, CD38-PE, HLA DR-APC monoclonal antibodies, then CD8⁺ TSCM cell percentage were determined by flow cytometry.To compare the difference of CD8⁺ TSCM cell percentage between HIV-1 chronic infected persons and healthy subjects, and to analyze the relationship between CD8⁺ TSCM cell percentage and disease progression index (CD4⁺ T cell count, HIV-1 viral load and immune activation index).Results Chronic HIV-1 infection resulted in a decrease of the CD8⁺ TSCM cell proportion in HIV-1 patients. CD8⁺ TSCM cells positively correlated with CD4⁺ T cell counts and negatively correlated with plasma viral load and CD8⁺ T cell activation. Prolonged ART successfully recovered the CD8⁺ TSCM cells, and the dynamic change of CD8⁺ TSCM cells was in parallel with CD4⁺ T cell restoration and a decrease in the level of T cell immune activation. Conclusion In summary, this report identifies CD8⁺ TSCM as a correlate of protection from disease progression. HIV-1-specific CD8 ⁺ TSCM can presumably directly contribute to the design of T cell-based vaccines.

Key words: human immunodeficiency virus, stem memory T cells, disease progression

中图分类号:

R512

宋冰冰, 陆小凡, 翁文佳, 粟斌, 张彤, 高艳青. HIV-1慢性感染者外周血中CD8⁺干细胞样记忆型T细胞变化以及对疾病进展的影响[J]. 首都医科大学学报, 2017, 38(5): 650-653.

Song Bingbing, Lu Xiaofan, Weng Wenjia, Su Bin, Zhang Tong, Gao Yanqing. Dynamic changes of CD8⁺ stem memory T cells and their effects on disease progression in chronic HIV-1 infection[J]. Journal of Capital Medical University, 2017, 38(5): 650-653.

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HIV-1慢性感染者外周血中CD8⁺干细胞样记忆型T细胞变化以及对疾病进展的影响

Dynamic changes of CD8⁺ stem memory T cells and their effects on disease progression in chronic HIV-1 infection

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HIV-1慢性感染者外周血中CD8+干细胞样记忆型T细胞变化以及对疾病进展的影响

Dynamic changes of CD8+ stem memory T cells and their effects on disease progression in chronic HIV-1 infection

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HIV-1慢性感染者外周血中CD8⁺干细胞样记忆型T细胞变化以及对疾病进展的影响

Dynamic changes of CD8⁺ stem memory T cells and their effects on disease progression in chronic HIV-1 infection