早幼粒白血病在银屑病病人外周血淋巴细胞中的表达及其与p53和bcl-2家族的相关性

doi:10.3969/j.issn.1006-7795.2017.05.003

首都医科大学学报 ›› 2017, Vol. 38 ›› Issue (5): 645-649.doi: 10.3969/j.issn.1006-7795.2017.05.003

• 皮肤病性病诊疗与研究 • 上一篇下一篇

早幼粒白血病在银屑病病人外周血淋巴细胞中的表达及其与p53和bcl-2家族的相关性

彭世光, 李娜, 蓝祖连, 李莉, 何焱玲

首都医科大学附属北京朝阳医院皮肤科, 北京 100020

收稿日期:2017-05-09 出版日期:2017-09-21 发布日期:2017-10-18
通讯作者: 何焱玲 E-mail:heyanling@medmail.com.cn
基金资助:
北京市自然科学基金项目（7102060）。

Overexpression of promyelocytic leukaemia gene correlated with p53 and bcl-2 family in psoriatic peripheral lymphocytes

Peng Shiguang, Li Na, Lan Zulian, Li Li, He Yanling

Department of Dermatology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China

Received:2017-05-09 Online:2017-09-21 Published:2017-10-18
Supported by:
This study was supported by Natural Science Foundation of Beijing(7102060).

摘要/Abstract

摘要： 目的研究早幼粒白血病（promyelocytic leukaemia，PML）、p53、B淋巴细胞瘤-2（B-cell lymphoma-2，bcl-2）和bcl-2相关X蛋白（bcl-2 associated X protein，bax）在银屑病外周血淋巴细胞中的表达，并评估上述因子之间的相关性。方法分离25例银屑病病人和20例健康对照的外周血淋巴细胞，通过qRT-PCR和流式细胞学分别检测PML、p53、bcl-2和bax在外周学淋巴细胞中mRNA和蛋白的表达水平。采用Pearson相关性分析评估PML与上述因子之间的相关性。结果与健康对照相比，银屑病病人外周血淋巴细胞中PML和p53的mRNA及蛋白表达增加，bcl-2的mRNA及蛋白表达下降，bax的mRNA表达下降但蛋白表达增加。PML蛋白表达与p53、bcl-2以及bax蛋白表达呈相关性。结论 PML可能参与了银屑病发病过程。

关键词: 早幼粒白血病, 银屑病, p53, Bcl-2家族, 凋亡

Abstract: Objective To investigate the expressions of promyelocytic leukaemia (PML) gene, p53, bcl-2 and bcl-2 associated X protein (bax) in psoriatic peripheral lymphocytes, and evaluate the relationships between PML and those candidate proteins. Methods Twenty-five psoriasis patients and 20 healthy donors were recruited. The peripheral lymphocytes were isolated. The mRNA and protein levels of PML, p53, bcl-2 and bax in peripheral lymphocytes were detected by qRT-PCR and flow cytometry, respectively. Pearson correlation matrix analysis was conducted to evaluate the relationships between PML protein and those candidate proteins.Results The mRNA and protein of PML and p53 were increased, while protein of bcl-2 decreased in peripheral lymphocytes of psoriasis patients as compared to healthy blood donors. The mRNA of bax decreased while protein of bax increased in psoriatic peripheral lymphocytes. The expression of PML protein was correlated with p53, bcl-2 and bax proteins in psoriatic peripheral lymphocytes. Conclusion PML may play a role in the mechanism of psoriasis.

Key words: promyelocytic leukaemia (PML), psoriasis, p53, Bcl-2 family, apoptosis

中图分类号:

R758.63

彭世光, 李娜, 蓝祖连, 李莉, 何焱玲. 早幼粒白血病在银屑病病人外周血淋巴细胞中的表达及其与p53和bcl-2家族的相关性[J]. 首都医科大学学报, 2017, 38(5): 645-649.

Peng Shiguang, Li Na, Lan Zulian, Li Li, He Yanling. Overexpression of promyelocytic leukaemia gene correlated with p53 and bcl-2 family in psoriatic peripheral lymphocytes[J]. Journal of Capital Medical University, 2017, 38(5): 645-649.

参考文献

[1] Deng Y, Chang C, Lu Q. The inflammatory response in psoriasis:a comprehensive review[J]. Clin Rev Allergy Immunol,2016, 50(3):377-389.
[2] Wang Y, Wang L L, Yang H Y, et al. Interleukin-21 is associated with the severity of psoriasis vulgaris through promoting CD4⁺ T cells to differentiate into Th17 cells[J]. Am J Transl Res,2016, 8(7):3188-3196.
[3] Wang Y, Mi Y, Li D, et al. Acute leukemia association with psoriasis:a report on 100 patients from a single center in China[J]. Am J Hematol, 2010, 85(5):378-379.
[4] Wong T, Hsu L, Liao W. Phototherapy in psoriasis:a review of mechanisms of action[J]. J Cutan Med Surg, 2013, 17(1):6-12.
[5] Carracedo A, Weiss D, Leliaert A K, et al. A metabolic prosurvival role for PML in breast cancer[J]. J Clin Invest, 2012, 122(9):3088-3100.
[6] Rokudai S, Laptenko O, Arnal S M, et al. MOZ increases p53 acetylation and premature senescence through its complex formation with PML[J]. Proc Natl Acad Sci U S A, 2013, 110(10):3895-3900.
[7] Hoetelmans R W. Nuclear partners of Bcl-2:Bax and PML[J]. DNA Cell Biol, 2004, 23(6):351-354.
[8] Salomoni P, Dvorkina M, Michod D. Role of the promyelocytic leukaemia protein in cell death regulation[J]. Cell Death Dis, 2012, 3:e247.
[9] Yeung P L, Denissova N G, Nasello C,et al. Promyelocytic leukemia nuclear bodies support a late step in DNA double-strand break repair by homologous recombination[J]. J Cell Biochem, 2012, 113(5):1787-1799.
[10] Yen C C, Tsao Y P, Chen P C, et al. PML protein as a prognostic molecular marker for patients with esophageal squamous cell carcinomas receiving primary surgery[J]. J Surg Oncol, 2011, 103(8):761-767.
[11] Yazici A C, Karabulut A A, Ozen O,et al. Expression of p53 in lesions and unaffected skin of patients with plaque-type and guttate psoriasis:a quantitative comparative study[J]. J Dermatol, 2007, 34(6):367-374.
[12] Ivanschitz L, Takahashi Y, Jollivet F,et al. PML IV/ARF interaction enhances p53 SUMO-1 conjugation, activation, and senescence[J]. Proc Natl Acad Sci U S A, 2015, 112(46):14278-14283.
[13] Pinton P, Giorgi C, Pandolfi P P. The role of PML in the control of apoptotic cell fate:a new key player at ER-mitochondria sites[J]. Cell Death Differ, 2011, 18(9):1450-1456.
[14] Haupt S, Mitchell C, Corneille V, et al. Loss of PML cooperates with mutant p53 to drive more aggressive cancers in a gender-dependent manner[J]. Cell Cycle, 2013, 12(11):1722-1731.
[15] Batinac T, Zamolo G, Hadzisejdić I, et al. Expression of Bcl-2 family proteins in psoriasis[J]. Croat Med J, 2007, 48(3):319-326.
[16] Myś Liwiec H, Flisiak I, Baran A,et al. Evaluation of CD40, its ligand CD40L and Bcl-2 in psoriatic patients[J]. Folia Histochem Cytobiol, 2012, 50(1):75-79.
[17] 张晓岚, 陈洋,姜奕.进展期寻常型银屑病病人皮损中IL-22的表达[J].中国医科大学学报,2015,44(4):362-364.
[18] 史雅琼,马建伟,庞晓文,等.银屑病合并代谢综合征病人血hs-CRP、IL-6水平及理化指标变化的意义[J].解放军医药杂志,2015,27(9):21-23.

早幼粒白血病在银屑病病人外周血淋巴细胞中的表达及其与p53和bcl-2家族的相关性

Overexpression of promyelocytic leukaemia gene correlated with p53 and bcl-2 family in psoriatic peripheral lymphocytes

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	张莹, 刘超楠, 时红林, 刘芳, 陈德喜, 时红波. P53凋亡刺激蛋白2介导酒精性肝病脂代谢相关基因的转录组学分析[J]. 首都医科大学学报, 2023, 44(1): 85-92.
[2]	刘志斌, 靳松, 牛亦农. 苯乙基异硫氰酸酯对良性前列腺上皮细胞增殖凋亡的影响及相关机制研究[J]. 首都医科大学学报, 2021, 42(6): 961-966.
[3]	安松林, 马玲, 赵月, 李雁. 主要促进因子超家族成员MFSD2A对肝癌细胞增殖、凋亡和侵袭的影响[J]. 首都医科大学学报, 2021, 42(6): 1000-1006.
[4]	崔兴, 钟萍. 我的梦想是做“百年老店”——记首都医科大学三博脑科医院神经外科于春江教授[J]. 首都医科大学学报, 2021, 42(1): 167-169.
[5]	郭肖瑶, 底婷婷, 赵京霞, 王燕, 解欣然, 翟春艳, 刘正荣, 蒙玉娇, 刘宇, 郭简宁, 张璐, 王宁, 张蕾, 李萍. 搜风顺气丸对咪喹莫特诱导的银屑病样小鼠γδT17的抑制作用[J]. 首都医科大学学报, 2019, 40(6): 881-888.
[6]	刘妍, 陈玉静, 陈文强, 黄小波. 远志汤对慢性脑低灌注大鼠认知功能及海马凋亡蛋白的影响[J]. 首都医科大学学报, 2019, 40(3): 323-329.
[7]	刘欣, 张广中, 姜春燕, 肖士菊, 谭勇, 曲剑华, 陈妍霏. 肠道微生态与银屑病的中医研究[J]. 首都医科大学学报, 2019, 40(3): 363-368.
[8]	张怡尘, 曹曦, 杨金奎. G蛋白偶联受体MAS对肝脏糖脂代谢的影响[J]. 首都医科大学学报, 2019, 40(3): 409-416.
[9]	卢晶, 沈涵, 程呈, 刘敬怡, 朱晓蓉, 谢荣荣, 袁明霞, 杨金奎. 人ether-α-go-go钾通道减少肝脏的内质网应激和凋亡[J]. 首都医科大学学报, 2019, 40(1): 45-52.
[10]	宋丽妮, 曹曦, 刘薇, 杨金奎. 血管紧张素转化酶2对肝细胞凋亡的影响[J]. 首都医科大学学报, 2018, 39(2): 223-229.
[11]	翟春艳, 底婷婷, 赵京霞, 王燕, 解欣然, 李雪, 蒙玉娇, 李宁飞, 刘正荣, 张璐, 张蕾, 王宁, 李萍. 解毒药与凉血药配伍对咪喹莫特诱导的银屑病样小鼠模型的干预作用[J]. 首都医科大学学报, 2018, 39(2): 243-251.
[12]	金双, 高连印, 赵梦培, 车念聪, 张秋云, 杜宇琼. 清毒汤对实验性重症肝损伤大鼠Bax蛋白及Bcl-2蛋白表达的影响[J]. 首都医科大学学报, 2018, 39(1): 79-83.
[13]	郭轶先, 张蓝方, 孙雪静, 万岁桂, 夏长青. 紫外线照射未成熟树突状细胞诱导小鼠同种抗原免疫耐受及其机制研究[J]. 首都医科大学学报, 2017, 38(3): 431-438.
[14]	史婷婷, 杨芳远, 曹曦, 卢晶, 谢荣荣, 袁明霞, 杨金奎. 血管紧张素转化酶2减少胰岛细胞的氧化应激及凋亡[J]. 首都医科大学学报, 2017, 38(2): 151-155.
[15]	王明星, 王燕, 赵京霞, 底婷婷, 阮智通, 蒙玉娇, 谢湘江, 张璐, 林燕, 王宁, 李萍. 树突状细胞在咪喹莫特诱导的银屑病样小鼠模型中的表达及凉血解毒方的干预作用[J]. 首都医科大学学报, 2017, 38(2): 249-259.