心力衰竭大鼠肺脏α1A及β1、β2肾上腺素受体表达的改变

doi:10.3969/j.issn.1006-7795.2013.01.027

首都医科大学学报 ›› 2013, Vol. 34 ›› Issue (1): 140-143.doi: 10.3969/j.issn.1006-7795.2013.01.027

心力衰竭大鼠肺脏α_1A及β₁、β₂肾上腺素受体表达的改变

蒋志丽¹, 李艳芳¹, 曹芳芳², 刘飞¹, 白雪源³, 吕杨³

1. 首都医科大学附属北京安贞医院心内科, 北京 100029;
2. 中国医学科学院北京协和医学院阜外心血管病医院心外科, 北京 100037;
3. 解放军总医院肾科, 北京 100853

收稿日期:2012-05-08 出版日期:2013-02-21 发布日期:2013-02-25
通讯作者: 李艳芳 E-mail:liyf101@sohu.com
基金资助:
中国中西医结合学会科研基金(2011-01)。

Expression of α_1A, β₁ and β₂ adrenergic receptors in rats lung with heart failure induced by myocardial infarction

JIANG Zhili¹, LI Yanfang¹, CAO Fangfang², LIU Fei¹, BAI Xueyuan³, LÜ Yang³

1. Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China;
2. Cardiac Surgery, Cardiovascular Institute and Fuwai Hospital, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100037, China;
3. Department of Nephrology, The General Hospital of The People Liberation Army, Beijing 100853, China

Received:2012-05-08 Online:2013-02-21 Published:2013-02-25
Supported by:
This study was supported by Chinese Integrated TCM-WM Scientific Research Foundation (2011-01).

摘要/Abstract

摘要：

目的研究慢性心力衰竭(chronic heart failure, CHF)时肺脏α_1A-肾上腺素受体(α_1A-AR)和β₁、β₂肾上腺素受体(β-AR)表达水平的变化。方法结扎Wistar大鼠左冠状动脉前降支制作心肌梗死后心力衰竭模型,模型成功后采用数字表法随机分为正常对照组、假手术组和心力衰竭模型组。取大鼠肺组织,采用蛋白质免疫印迹法(Western blotting)分别测定3组的α_1A-AR和β₁、β₂-AR蛋白表达水平。结果心力衰竭模型组与假手术组相比,α_1A-AR、β₁-AR的蛋白表达水平显著下调(P<0.01或P<0.05),β₂-AR的蛋白表达水平显著上调(P<0.05),正常对照组α_1A-AR、β₁-AR和β₂-AR的蛋白表达水平与假手术组相比差异无统计学意义(P>0.05)。结论 CHF大鼠肺循环淤血,α_1A-AR下调,有利于肺血管舒张,抑制平滑肌增生以代偿这种病理改变。肺脏β₂-AR表达水平上调可能与β₂-AR激动可以舒张血管和支气管平滑肌作用,开放钠离子通道等机制促进肺水肿液的清除、并能改善心力衰竭条件下肺脏微血管的通透性有关。心力衰竭时肺脏β₁-AR的下调一方面与CHF时交感肾上腺素系统激活产生的负反馈调节有关,另一面可能与代偿性拮抗β₂-AR的上调,维持相对稳定的肺泡液体清除率有关。

关键词: 肾上腺素受体, 肺脏, 心力衰竭

Abstract:

Objective To investigate the protein levels of α_1A,β₁ and β₂ adrenergic receptor ( AR ) in lungs of rats with chronic heart failure (CHF) induced by myocardial infarction. Methods Models of CHF established by anterior descending coronary artery ligature. Thirty five Wistar rats were randomly divided into three groups: control group (group A), sham operation group (group B) and CHF model group (group C). The protein expression levels of α_1A-AR, β₁-AR and β₂-AR in lung tissue were measured by Western blotting.Results Compared with sham operation group,α_1A-AR, β₁-AR protein expression in group C markedly decreased (P<0.01 and P<0.05), expression of β₂-AR remarkably increased (P<0.05), while there was no significant difference between control group and sham operation group (P>0.05). Conclusion CHF rats always had pulmonary congestion, the α_1A-AR down-regulation helped pulmonary vascular relax and inhibited smooth muscle cells proliferation, which was a compensation of the pathological situation of CHF lung. Increasing expression of β₂-AR in CHF lungs could relax the vascular and bronchial smooth muscle; promote the clearance of pulmonary edema fluid and lung conditions through opening the sodium ion channels and other mechanisms. It could also improve the permeability of the capillaries in lungs. The expression of β₁-AR in CHF rats was down-regulated for negative feedback regulation of activation of the sympathetic adrenergic system or compensation of up-regulation of β₂-AR,which could maintain a relatively stable rates of alveolar fluid clearance.

Key words: adrenergic receptor, lung, heart failure

中图分类号:

R541.6

蒋志丽, 李艳芳, 曹芳芳, 刘飞, 白雪源, 吕杨. 心力衰竭大鼠肺脏α_1A及β₁、β₂肾上腺素受体表达的改变[J]. 首都医科大学学报, 2013, 34(1): 140-143.

JIANG Zhili, LI Yanfang, CAO Fangfang, LIU Fei, BAI Xueyuan, LÜ Yang. Expression of α_1A, β₁ and β₂ adrenergic receptors in rats lung with heart failure induced by myocardial infarction[J]. Journal of Capital Medical University, 2013, 34(1): 140-143.

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心力衰竭大鼠肺脏α_1A及β₁、β₂肾上腺素受体表达的改变

Expression of α_1A, β₁ and β₂ adrenergic receptors in rats lung with heart failure induced by myocardial infarction

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