麝香保心丸对心力衰竭大鼠肾脏血管紧张素Ⅱ受体各亚型表达的影响

doi:10.3969/j.issn.1006-7795.2012.01.020

首都医科大学学报 ›› 2012, Vol. 33 ›› Issue (1): 99-102.doi: 10.3969/j.issn.1006-7795.2012.01.020

麝香保心丸对心力衰竭大鼠肾脏血管紧张素Ⅱ受体各亚型表达的影响

刘飞¹, 李艳芳¹, 曹芳芳¹, 张玲姬¹, 白雪源², 吕杨²

1. 首都医科大学附属北京安贞医院心内科, 北京 100029;2. 解放军总医院肾科, 北京 100039

收稿日期:2011-09-29 修回日期:1900-01-01 出版日期:2012-02-21 发布日期:2012-02-21
通讯作者: 李艳芳

Effect of Shexiangbaoxin pills on expression of renal angiotensin Ⅱ receptor subtypes in rats with heart failure

LIU Fei¹, LI Yan-fang¹, CAO Fang-fang¹, ZHANG Ling-ji¹, BAI Xue-yuan², LV Yang²

1. Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China;2. Department of Nephrology, Chinese PLA General Hospital, Beijing 100039, China

Received:2011-09-29 Revised:1900-01-01 Online:2012-02-21 Published:2012-02-21

摘要/Abstract

摘要： 目的探讨麝香保心丸对心力衰竭大鼠肾脏血管紧张素Ⅱ受体(angiotensin Ⅱ receptor, ATR)各亚型表达水平的影响。方法选择雄性Wistar大鼠80只,采用抽签法随机分为正常对照组(A组,10只)、假手术组(B组,10只),其余60只制作心肌梗死后心力衰竭模型,35只大鼠模型制作成功,并采用抽签法随机分为模型组(C组,9只)、阳性药物对照组(D组,8只)、麝香保心丸小剂量组(E组,9只)和麝香保心丸大剂量组(F组,9只)。取大鼠肾脏皮质,采用反转录聚合酶链反应(RT-PCR)法分别测定各组大鼠ATR各亚型mRNA表达水平。结果 B组与A组AT₁R、AT₂R比较,差异无统计学意义(P>0.05);与B组比较,C组大鼠AT₁R表达明显上调,AT₂R表达明显下调,差异均有统计学意义(P<0.05);与C组比较,D、E、F组大鼠AT₁R表达明显下调,AT₂R表达明显上调,差异有统计学意义(P<0.01);与E组比较,F组大鼠AT₁R表达下调更显著(P<0.05)。结论采用麝香保心丸治疗,可调节大鼠肾脏ATR各亚型的表达,使心力衰竭时上调的AT₁R表达下调,下调的AT₂R表达上调,对保护肾脏功能、改善心力衰竭起到了有益作用。同时,与小剂量麝香保心丸相比,大剂量麝香保心丸的疗效更为显著。

关键词: 麝香保心丸, 血管紧张素Ⅱ受体, 肾脏, 心力衰竭

Abstract: Objective To explore the effect of Shexiangbaoxin pills (SXBXP) on expression of renal angiotensin Ⅱ receptor(ATR) subtypes in rats with heart failure.Methods Eighty male Wistar rats were randomly divided into control group (group A, n=10) and sham operation group (group B, n=10). The heart failure model was established successfully in 35 of the rest 60 male Wistar rats. The 35 heart failure model rats were randomly divided into four group: heart failure model group (group C, n=9), medicine control group (group D, n=8), small dose of SXBXP group (group E, n=9) and large dose of SXBXP group (group F, n=9). The mRNA levels of ATR subtypes of renal cortex were measured by reverse transcription polymerase chain reaction (RT-PCR) in each group. Results Compared with group A, expression of AT₁R and AT₁R showed no significant difference in group B(P>0.05), compared with group B, expression of AT₁R was increased significantly in group C(P<0.05), expression of AT₁R was decreased significantly in group C(P<0.01). Compared with group C, expression of AT₁R was decreased significantly in group D, E and F(P<0.05), expression of AT₁R was increased significantly in group D, E and F(P<0.01). Compared with group E, expression of AT₁R was decreased significantly in group F(P<0.05). Conclusion SXBXP regulated the expression of renal ATR subtypes, including the downregulation of AT₁R and the upregulation of AT₁R in rats with heart failure, and it is also effective to protect renal function and improve heart failure, especially large dose of SXBXP.

Key words: Shexiangbaoxin pills, angiotensin Ⅱ receptor, kidney, heart failure

中图分类号:

R 54

刘飞;李艳芳;曹芳芳;张玲姬;白雪源;吕杨. 麝香保心丸对心力衰竭大鼠肾脏血管紧张素Ⅱ受体各亚型表达的影响[J]. 首都医科大学学报, 2012, 33(1): 99-102.

LIU Fei;LI Yan-fang;CAO Fang-fang;ZHANG Ling-ji;BAI Xue-yuan;LV Yang. Effect of Shexiangbaoxin pills on expression of renal angiotensin Ⅱ receptor subtypes in rats with heart failure[J]. Journal of Capital Medical University, 2012, 33(1): 99-102.

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麝香保心丸对心力衰竭大鼠肾脏血管紧张素Ⅱ受体各亚型表达的影响

Effect of Shexiangbaoxin pills on expression of renal angiotensin Ⅱ receptor subtypes in rats with heart failure

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