不同浓度β1-肾上腺素受体自身抗体对心肌细胞存活影响的差异性

doi:10.3969/j.issn.1006-7795.2019.03.015

首都医科大学学报 ›› 2019, Vol. 40 ›› Issue (3): 402-408.doi: 10.3969/j.issn.1006-7795.2019.03.015

不同浓度β₁-肾上腺素受体自身抗体对心肌细胞存活影响的差异性

王兆佳^1,2, 武烨^1,2, 刘丹^2,3, 王美丽^1,2, 隗歆^1,2, 刘慧荣^1,2

1. 首都医科大学基础医学院生理学与病理生理学系, 北京 100069;
2. 代谢紊乱相关心血管疾病北京市重点实验室, 北京 100069;
3. 首都医科大学燕京医学院, 北京 101300

收稿日期:2018-12-22 出版日期:2019-05-21 发布日期:2019-06-13
通讯作者: 刘慧荣 E-mail:liuhr2000@126.com
基金资助:
国家自然科学基金（91539205），北京市自然科学基金（7192019）。

Differences in cardiomyocytes' survival by anti-β₁-adrenergic receptor autoantibodies of different concentrations

Wang Zhaojia^1,2, Wu Ye^1,2, Liu Dan^2,3, Wang Meili^1,2, Wei Xin^1,2, Liu Huirong^1,2

1. Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China;
2. Beijing Key Laboratory of Metabolic Disorders Related Cardiovascular Diseases, Beijing 100069, China;
3. Yanjing Medical College, Capital Medical University, Beijing 101300, China

Received:2018-12-22 Online:2019-05-21 Published:2019-06-13
Supported by:
This study was supported by National Natural Science Foundation of China (91539205),Natural Science Foundation of Beijing (7192019).

摘要/Abstract

摘要： 目的观察不同浓度的β₁-肾上腺素受体自身抗体（β₁-adrenergic receptor autoantibodies，β₁-AA）作用不同时间，对于心肌细胞产生的不同终末效应。方法分别使用10^-10、10^-9、10^-8、10^-7、10^-6 mol/L β₁-AA作用于乳鼠心肌细胞（neonatal rat cardiomyocytes，NRCMs）和H9c2细胞检测细胞生存率（24、36、48 h），作用于H9c2细胞检测线粒体功能（ATP含量、线粒体膜电势）（48 h）。结果中、高浓度（10^-7、10^-6 mol/L）β₁-AA刺激各个时间，NRCMs、H9c2细胞生存率明显降低；而低浓度（10^-10、10^-9、10^-8mol/L）使得H9c2细胞轻微增生。高浓度（10^-6 mol/L）β₁-AA刺激后，H9c2细胞中ATP含量降低，而中低浓度（10^-10、10^-9、10^-8、10^-7mol/L）β₁-AA使H9c2细胞中ATP含量增加。高浓度β₁-AA （10^-6 mol/L）刺激后，H9c2细胞线粒体膜电势降低，低浓度（10^-10、10^-8 mol/L）β₁-AA则使H9c2细胞线粒体膜电势增加。结论高浓度β₁-AA刺激心肌细胞后表现为损伤作用，低浓度刺激表现为正性作用（即增强线粒体功能、促存活），与作用时间无明显相关，本研究为β₁-AA阳性人群中心功能差异的现象提供了病理生理学依据。

关键词: β₁-AA, 心肌细胞, 线粒体

Abstract: Objective To investigate the terminal effects on cardiomyocytes of β₁-adrenergic receptor autoantibodies (β₁-AA) at different concentrations for different time. Methods Cell survival was detected after 10^-10, 10^-9, 10^-8, 10^-7, 10^-6 mol/L β₁-AA treating neonatal rat cardiomyocytes (NRCMs) and H9c2 cells for 24, 36, and 48 hours, while mitochondrial function (ATP content and mitochondrial membrane potential) of H9c2 cells was detected after 48 hours.Results The cell survival of NRCMs and H9c2 decreased significantly by β₁-AA in high-concentrations (10^-7, 10^-6 mol/L) at any time and the survival of H9c2 proliferated slightly in low concentrations (10^-10, 10^-9, 10^-8mol/L) at any time. ATP content of H9c2 was decreased by β₁-AA in high concentration (10^-6 mol/L), while increased in medium and low concentrations (10^-10, 10^-9, 10^-8, 10^-7 mol/L). The mitochondrial membrane potential of H9c2 was decreased by β₁-AA in high concentrations (10^-6 mol/L), while increased in low concentrations. Conclusion β₁-AA damage cardiomyocytes in high concentration while it showed the positive effects in low concentrations (ie, promoting ATP production and cell survival). And all the effects had no relation to time. In conclusion, our study provides a new foundation for the differences of cardiac function among β₁-AA positive population.

Key words: β₁-AA, cardiomyocyte, mitochondria

中图分类号:

R541.6

王兆佳, 武烨, 刘丹, 王美丽, 隗歆, 刘慧荣. 不同浓度β₁-肾上腺素受体自身抗体对心肌细胞存活影响的差异性[J]. 首都医科大学学报, 2019, 40(3): 402-408.

Wang Zhaojia, Wu Ye, Liu Dan, Wang Meili, Wei Xin, Liu Huirong. Differences in cardiomyocytes' survival by anti-β₁-adrenergic receptor autoantibodies of different concentrations[J]. Journal of Capital Medical University, 2019, 40(3): 402-408.

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不同浓度β₁-肾上腺素受体自身抗体对心肌细胞存活影响的差异性

Differences in cardiomyocytes' survival by anti-β₁-adrenergic receptor autoantibodies of different concentrations

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