用PCR法检测真菌性鼻窦炎分泌物中的真菌

首都医科大学学报 ›› 2005, Vol. 26 ›› Issue (3): 249-251.

用PCR法检测真菌性鼻窦炎分泌物中的真菌

周兵¹, 张伟², 孙文艳³, 陶格陶呼³, 杨凌¹

1. 首都医科大学附属北京同仁医院耳鼻咽喉-头颈外科;2. 北京市耳鼻咽喉科研究所;;3. 内蒙古医学院

收稿日期:2005-05-19 修回日期:1900-01-01 出版日期:2005-06-24 发布日期:2005-06-24

Detection of Fungi in Secretion of Fungal Sinusitis Using Polymerase Chain Reaction

Zhou Bing¹, Zhang Wei², Sun Wenyan³, Taogetaohu³, Yang Ling¹

1. Department of Otorhinolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital University of Medical Sciences;2. Beijing Institute of Otolaryngology;3. Inner Mongolia Medical College

Received:2005-05-19 Revised:1900-01-01 Online:2005-06-24 Published:2005-06-24

摘要/Abstract

摘要： 目的比较真菌内转录片段(ITS)PCR检测方法与培养的敏感性,探讨真菌ITS PCR检测方法的敏感性与特异性及其在真菌性鼻窦炎诊断中的意义.方法取18例临床诊断为真菌性鼻窦炎患者的鼻窦内分泌物,分别做真菌ITS PCR及培养,比较2种方法阳性率的差异及检出真菌的相符率.同时以20例临床诊断为非真菌性慢性鼻窦炎的鼻窦内分泌物为对照.结果 18例真菌性鼻窦炎的标本中,ITS PCR方法检测真菌阳性的例数为14例(78%);传统培养的阳性例数为8例(44%).20例慢性鼻窦炎ITS PCR方法检测阳性例数为1例(5%).结论 ITS PCR方法对于真菌性鼻窦炎的真菌病原快速诊断很有价值,并能通过测序明确感染菌种,是一种高敏感和高特异性的方法.

关键词: 真菌性鼻窦炎, 真菌, 聚合酶链反应

Abstract: Objective Polymerase chain reaction (PCR) of internal transcribed spacer region (ITS) is an exquisitely sensitive assay that can detect the DNA of fungal elements. The aim of this study was to compare the sensitivity of conventional culture and techniques using PCR. Methods The samples of secretion were collected from 18 patients diagnosed as fungal sinusitis clinically and 20 cases of control group with chronic sinusitis. Fungal-specific PCR analysis and standard culture were performed on every sample. χ² analysis was used to test for statistical differences between the two groups. Results ITSPCR analysis detected fungal DNA in fourteen(78%) patients with chronic fungal sinusitis and eight cases in control group while the positive results of standard cultures were 44%, respectively. Conclusion ITS PCR is more rapid and more sensitive technique than nasal swab culture in detecting the fungi in sinus secretion. The study suggested that it is an effective method in diagnosis of fungal sinusitis.

Key words: fungal sinusitis, fungi, PCR

中图分类号:

R765.22

周兵;张伟;孙文艳;陶格陶呼;杨凌. 用PCR法检测真菌性鼻窦炎分泌物中的真菌[J]. 首都医科大学学报, 2005, 26(3): 249-251.

Zhou Bing;Zhang Wei;Sun Wenyan;Taogetaohu;Yang Ling. Detection of Fungi in Secretion of Fungal Sinusitis Using Polymerase Chain Reaction[J]. Journal of Capital Medical University, 2005, 26(3): 249-251.

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