腹腔内注射脂多糖对大鼠黑质ROS含量及NADPH酶的影响

doi:10.3969/j.issn.1006-7795.2015.06.009

首都医科大学学报 ›› 2015, Vol. 36 ›› Issue (6): 882-886.doi: 10.3969/j.issn.1006-7795.2015.06.009

• 神经系统疾病的基础研究 • 上一篇下一篇

腹腔内注射脂多糖对大鼠黑质ROS含量及NADPH酶的影响

白丽娟¹, 姜新¹, 陈晓虹¹, 任艳², 罗晓光²

1. 辽宁省人民医院神经内科, 沈阳 110016;
2. 中国医科大学附属第一医院神经内科, 沈阳 110001

收稿日期:2015-10-10 出版日期:2015-12-21 发布日期:2015-12-18
通讯作者: 白丽娟 E-mail:blj_0510@sina.com
基金资助:
沈阳市科委基金(F14-158-9-07)。

Effect of intraperitoneal injection of lipopolysaccharides on the substantia nigra NADPH oxidase in rats

Bai Lijuan¹, Jiang Xin¹, Chen Xiaohong¹, Ren Yan², Luo Xiaoguang²

1. Department of Neurology, The People's Hospital of Liaoning Province, Shenyang 110016, China;
2. Department of Neurology, The First Hospital of China Medical University, Shenyang 110001, China

Received:2015-10-10 Online:2015-12-21 Published:2015-12-18
Supported by:
This study was supported by Shenyang Municipal Science and Technology Committee Foundation(F14-158-9-07).

摘要/Abstract

摘要： 目的观察腹腔注射脂多糖(lipopolysaccharide,LPS)后,大鼠黑质还原型烟酰胺腺嘌呤二核苷酸磷酸(nicotinamide-adenine dinucleotide phosphate,NADPH)氧化酶的表达及活性氧自由基(reactive oxygen species,ROS)的产生情况。方法健康2月龄、1年龄SD大鼠经腹腔注射LPS(1 mg/kg)后,分别于6 h、12 h、24 h、48 h、1周处死,快速取脑黑质,用DCFH-DA探针检测各组大鼠ROS产生,用Western blotting法检测NADPH酶在胞质蛋白和膜蛋白的表达情况。结果腹腔注射LPS 12 h,大鼠黑质内ROS活性达到高峰,NADPH酶活性增加,达到高峰。结论无论在老年组与青年组,LPS刺激后(12 h)的氧化应激反应均可能是通过激活NADPH氧化酶途径而发生的。

关键词: 脂多糖, 小胶质细胞, 活性氧自由基, NADPH氧化酶

Abstract: Objective To observe the expression of nicotinamide-adenine dinucleotide phosphate(NADPH) oxidase and the production of reactive oxygen species(ROS) in substantia nigra after intraperitoneal injection of lipopolysaccharide(LPS) in rats. Methods Healthy two-month-old and one-year-old SD rats were intraperitoneally injected with LPS(1 mg/kg) at 6 h, 12 h, 24 h, 48 h, and 1 week, respectively. Substantia nigra was taken, reactive oxygen species(ROS) and the activity of NADPH oxidase in the rats were determined. Results At 12 h after intraperitoneal injection with LPS, the activity of ROS in substantia nigra reached the peak and NADPH activity also reached the peak. Conclusion The increase in expression of NADPH oxidase and the shift to membranes protein promotes the inflammatory reaction, however there was no significant differences between the aged group and the young group.

Key words: lipopolysaccharide(LPS), microglia, reactive oxygen species(ROS), NADPH oxidase(PHOX)

中图分类号:

R742.5

白丽娟, 姜新, 陈晓虹, 任艳, 罗晓光. 腹腔内注射脂多糖对大鼠黑质ROS含量及NADPH酶的影响[J]. 首都医科大学学报, 2015, 36(6): 882-886.

Bai Lijuan, Jiang Xin, Chen Xiaohong, Ren Yan, Luo Xiaoguang. Effect of intraperitoneal injection of lipopolysaccharides on the substantia nigra NADPH oxidase in rats[J]. Journal of Capital Medical University, 2015, 36(6): 882-886.

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腹腔内注射脂多糖对大鼠黑质ROS含量及NADPH酶的影响

Effect of intraperitoneal injection of lipopolysaccharides on the substantia nigra NADPH oxidase in rats

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