载脂蛋白C3转基因小鼠应用于严重高三酰甘油血症急性胰腺炎的探索

doi:10.3969/j.issn.1006-7795.2020.03.018

首都医科大学学报 ›› 2020, Vol. 41 ›› Issue (3): 421-427.doi: 10.3969/j.issn.1006-7795.2020.03.018

载脂蛋白C3转基因小鼠应用于严重高三酰甘油血症急性胰腺炎的探索

柳鑫¹, 徐有青², 崔纯莹³, 赵志刚¹

1. 首都医科大学附属北京天坛医院药学部, 北京 100070;
2. 首都医科大学附属北京天坛医院消化内科, 北京 100070;
3. 首都医科大学药学院药剂学系, 北京 100069

收稿日期:2019-10-23 出版日期:2020-06-21 发布日期:2020-06-17
通讯作者: 赵志刚 E-mail:1022zzg@sina.com
基金资助:
北京市医院管理中心青苗培育项目（QML20170507），中共北京市委优秀人才项目（2018000021469G239），首都医科大学科研培育基金（17JL67）。

Application of ApoC3 transgenic mice in acute pancreatitis with severe hypertrig-lyceridemia

Liu Xin¹, Xu Youqing², Cui Chunying³, Zhao Zhigang¹

1. Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China;
2. Department of Gastroenterology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China;
3. Department of Pharmaceutics, College of Pharmacy, Capital Medical University, Beijing 100069, China

Received:2019-10-23 Online:2020-06-21 Published:2020-06-17
Supported by:
This study was supported by Beijing Municipal Administration of Hospitals' Youth Program (QML20170507), Talent project of Beijing Municipal Committee (2018000021469G239), Cultivation Fund of Capital Medical University (17JL67).

摘要/Abstract

摘要： 目的本研究旨在建立严重高三酰甘油血症下的急性胰腺炎小鼠模型。方法采用野生小鼠与载脂蛋白C3（apolipoprotein C3，ApoC3）转基因小鼠，以雨蛙素诱导急性胰腺炎，检测血浆脂质、淀粉酶活性与胰腺病理。并通过抗氧化剂叔丁基-特丁基对苯二酚（terbiary-butylhydroquinone，tBHQ）预防性给药，检测胰腺病理改变。结果 ApoC3转基因小鼠血浆三酰甘油浓度显著升高，在诱发急性胰腺炎后，淀粉酶活性在9 h与12 h时均升高，胰腺病理损伤加重较明显。使用tBHQ并不能改善ApoC3转基因小鼠的胰腺病理损伤。结论 ApoC3转基因小鼠诱导急性胰腺炎后，血浆淀粉酶活性增加，胰腺病理损伤明显加重，与临床中严重高三酰甘油血症-急性胰腺炎患者的胰腺病理改变类似，ApoC3转基因小鼠可以作为探讨严重高三酰甘油血症下急性胰腺炎病理机制研究的动物模型。

关键词: 严重高三酰甘油血症, 急性胰腺炎, 载脂蛋白C3转基因, 三酰甘油, 叔丁基-特丁基对苯二酚

Abstract: Objective To establish acute pancreatitis mouse model with severe hypertriglyceridemia. Methods Wild type and apolipoprotein C3 (ApoC3) transgenic mice were used to induce acute pancreatitis with caerulein. Plasma levels of lipids, amylase activity and pancreatic pathology were detected. The pancreatic pathological changes were also evaluated by administrating antioxidant terbiary-butylhydroquinone (tBHQ). Results ApoC3 transgenic mice demonstrated significantly higher plasma levels of triglyceride, and aggravated pancreatic pathological injury after inducing acute pancreatitis. Amylase activity in ApoC3 transgenic mice increased at 9h and 12h. The treatment of tBHQ did not improve pancreatic pathology in ApoC3 transgenic mice. Conclusions ApoC3 transgenic mice showed increased plasma amylase activity, and significantly aggravated pancreatic pathological injury after inducing acute pancreatitis. These were similar to pathological changes of pancreas in clinical patients with acute pancreatitis in severe hypertriglyceridemia. ApoC3 transgenic mice could be used as a mouse model for studying pathological mechanism of acute pancreatitis in severe hypertriglyceridemia.

Key words: severe hypertriglyceridemia, acute pancreatitis, apolipoprotein C3 (ApoC3) transgenic, triglyceride, terbiary-butylhydroquinone (tBHQ)

中图分类号:

柳鑫, 徐有青, 崔纯莹, 赵志刚. 载脂蛋白C3转基因小鼠应用于严重高三酰甘油血症急性胰腺炎的探索[J]. 首都医科大学学报, 2020, 41(3): 421-427.

Liu Xin, Xu Youqing, Cui Chunying, Zhao Zhigang. Application of ApoC3 transgenic mice in acute pancreatitis with severe hypertrig-lyceridemia[J]. Journal of Capital Medical University, 2020, 41(3): 421-427.

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载脂蛋白C3转基因小鼠应用于严重高三酰甘油血症急性胰腺炎的探索

Application of ApoC3 transgenic mice in acute pancreatitis with severe hypertrig-lyceridemia

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