电针对帕金森病模型小鼠纹状体背侧的c-Fos表达的选择性调节

doi:10.3969/j.issn.1006-7795.2021.06.014

首都医科大学学报 ›› 2021, Vol. 42 ›› Issue (6): 986-992.doi: 10.3969/j.issn.1006-7795.2021.06.014

电针对帕金森病模型小鼠纹状体背侧的c-Fos表达的选择性调节

粟文婷¹, 於佳楠², 贾军³, 王晓民^2,3*

1.首都医科大学脑重大疾病研究中心北京脑重大疾病研究院北京市脑重大疾病重点实验室——省部共建国家重点实验室培育基地脑重大疾病防治省部共建协同创新中心,北京 100069;
2.首都医科大学基础医学院神经生物学系,北京 100069;
3.首都医科大学基础医学院生理学与病理生理学系,北京 100069

收稿日期:2021-07-16 出版日期:2021-12-21 发布日期:2021-12-17
基金资助:
国家自然科学基金(81704151,81473770),北京市教育委员会科技计划一般项目(KM201710025014)。

Electroacupuncture selectively regulates the expression of c-Fos in the dorsal striatum of Parkinsonian mice

Su Wenting¹, Yu Jianan², Jia Jun³, Wang Xiaomin^2,3*

1. Beijing Institute of Brain Disorders, Laboratory of Brain Disorders, Ministry of Science and Technology, Collaborative Innovation Center for Brain Disorders, Capital Medical University, Beijing 100069, China;
2. Department of Neurobiology,School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China;
3. Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2021-07-16 Online:2021-12-21 Published:2021-12-17
Contact: * E-mail: xmwang@ccmu.edu.cn
Supported by:
National Natural Science Foundation of China (81704151,81473770),Scientific Research Common Program of Beijing Municipal Commission of Education (KM201710025014).

摘要/Abstract

摘要： 目的观察电针对帕金森病(Parkinson’s disease, PD)模型小鼠纹状体内原癌基因蛋白(c-Fos)表达的选择性调控作用,以探究电针治疗PD的潜在机制。方法制备6-羟基多巴胺(6-hydroxydopamine, 6-OHDA)右侧损伤的PD模型小鼠,实验分为对照组、对照+电针组、模型组和模型+电针组。电针施加于小鼠下肢双侧的“足三里”和“三阴交”穴位,采用100 Hz电针刺激持续30 min,继而用免疫组化检测纹状体背侧、腹侧和扣带回皮质的c-Fos蛋白表达。结果模型组小鼠右侧背部纹状体c-Fos表达较对照组显著降低(P<0.001),而电针刺激显著增加了模型组右侧背部和腹部纹状体的c-Fos的表达(P<0.05)。检测扣带回皮质的c-Fos表达,各组之间差异无统计学意义,电针未产生明显的调控作用。结论电针可区域特异性地激活PD小鼠损伤侧纹状体背部神经元的活性。

关键词: 电针, 帕金森病, 纹状体, c-Fos表达

Abstract: Objective To observe the selective regulation of electroacupuncture (EA) on the expression of proto oncogene protein (c-Fos) in the striatum of Parkinsonian mice, so as to explore the potential mechanism of EA in the treatment of Parkinson's disease (PD). Methods PD model mice with right side injury by 6-hydroxydopamine (6-OHDA) were made. The mice were divided into Sham, Sham+EA, Model and Model + EA groups. The “Zusanli” and “Sanyinjiao” acupoints on both sides of the lower limbs of mice were used as the stimulation sites. 100 Hz EA was applied for 30 minutes, and the c-Fos protein expression in the dorsal and ventral striatum and cingulate cortex were detected by immunohistochemistry later. Results Compared with the Sham group, the expression of c-Fos in the right dorsal side of the Model mice was significantly lower (P<0.001), and EA stimulation increased the expression of c-Fos in the right dorsal and ventral side of the Model group (P<0.05). In the cingulate cortex, there was no significant difference in the expression of c-Fos among these groups, and EA had no obvious regulatory effect. Conclusion EA can specifically activate the activity of neurons in the dorsal striatum on the injured side of parkinsonian mice.

Key words: electroacupuncture, Parkinson's disease, striatum, c-Fos expression

中图分类号:

R245.3

粟文婷, 於佳楠, 贾军, 王晓民. 电针对帕金森病模型小鼠纹状体背侧的c-Fos表达的选择性调节[J]. 首都医科大学学报, 2021, 42(6): 986-992.

Su Wenting, Yu Jianan, Jia Jun, Wang Xiaomin. Electroacupuncture selectively regulates the expression of c-Fos in the dorsal striatum of Parkinsonian mice[J]. Journal of Capital Medical University, 2021, 42(6): 986-992.

参考文献

[1] Bloem B R, Okun M S, Klein C. Parkinson's disease[J]. Lancet, 2021, 397(10291): 2284-2303.
[2] Guo X Y, Ma T. Effects of acupuncture on neurological disease in clinical- and animal-based research[J]. Front Integr Neurosci, 2019, 13: 47.
[3] Zhao Y D, Zhang Z C, Qin S R, et al. Acupuncture for Parkinson's disease: efficacy evaluation and mechanisms in the dopaminergic neural circuit[J]. Neural Plast, 2021, 2021: 9926445.
[4] Liang X B, Luo Y, Liu X Y, et al. Electro-acupuncture improves behavior and upregulates GDNF mRNA in MFB transected rats[J]. Neuroreport, 2003, 14(8): 1177-1181.
[5] Wang H M, Pan Y L, Xue B, et al. The antioxidative effect of electro-acupuncture in a mouse model of Parkinson's disease[J]. Plos One, 2011, 6(5): e19790.
[6] Jia J, Sun Z L, Li B, et al. Electro-acupuncture stimulation improves motor disorders in Parkinsonian rats[J]. Behav Brain Res, 2009, 205(1): 214-218.
[7] Lv E, Deng J, Yu Y, et al. Nrf2-ARE signals mediated the anti-oxidative action of electroacupuncture in an MPTP mouse model of Parkinson's disease[J]. Free Radic Res, 2015, 49(11): 1296-1307.
[8] Deng J H, Lv E, Yang J, et al. Electroacupuncture remediates glial dysfunction and ameliorates neurodegeneration in the astrocytic α-synuclein mutant mouse model[J]. J Neuroinflammation, 2015, 12: 103.
[9] Yu Y, Wang K, Deng J H, et al. Electroacupuncture produces the sustained motor improvement in 6-hydroxydopamine-lesioned mice[J]. Plos One, 2016, 11(2): e0149111.
[10] Jia Y J, Deng J H, Zhang W Z, et al. The role of group II metabotropic glutamate receptors in the striatum in electroacupuncture treatment of Parkinsonian rats[J]. CNS Neurosci Ther, 2017, 23(1): 23-32.
[11] Li M, Li L J, Wang K, et al. The effect of electroacupuncture on proteomic changes in the motor cortex of 6-OHDA Parkinsonian rats[J]. Brain Res, 2017, 1673: 52-63.
[12] Bullitt E. Expression of c-fos-like protein as a marker for neuronal activity following noxious stimulation in the rat[J]. J Comp Neurol, 1990, 296(4): 517-530.
[13] 李莉,余剑波,刘志学. 针刺镇痛与c-fos基因的研究[J]. 临床麻醉学杂志, 2011, 27(10): 1030-1032.
[14] 于燕,王可,贾军,等. 纹状体注射不同剂量6-OHDA制备帕金森病小鼠模型的评价[J]. 首都医科大学学报, 2015, 36(2): 255-261.
[15] DeLong M R. Primate models of movement disorders of basal ganglia origin[J]. Trends Neurosci, 1990, 13(7): 281-285.
[16] Meoni S, Cury R G, Moro E. New players in basal ganglia dysfunction in Parkinson's disease[J]. Prog Brain Res, 2020, 252: 307-327.
[17] Fieblinger T. Striatal control of movement: a role for new neuronal (sub-) populations?[J]. Front Hum Neurosci, 2021, 15: 697284.
[18] 张辉,马惠清,王森茂,等. 银杏叶提取物对帕金森病模型大鼠脑内神经炎性反应的影响[J]. 首都医科大学学报, 2019, 40(3): 439-442.
[19] Cao L J, Li X X, Li M X, et al. The effectiveness of acupuncture for Parkinson’s disease: an overview of systematic reviews[J]. Complement Ther Med, 2020, 50: 102383.
[20] Lee S H, Lim S. Clinical effectiveness of acupuncture on Parkinson disease: a PRISMA-compliant systematic review and meta-analysis[J]. Medicine, 2017, 96(3): e5836.
[21] Ye Q P, Liu C Y, Shi J H, et al. Effect of electro-acupuncture on regulating the swallowing by activating the interneuron in ventrolateral medulla (VLM)[J]. Brain Res Bull, 2019, 144: 132-139.
[22] Shi J H, Ye Q P, Zhao J Y, et al. EA promotes swallowing via activating swallowing-related motor neurons in the nucleus ambiguus[J]. Brain Res, 2019, 1718: 103-113.
[23] 吴锋, 缪化春, 李怀斌. 电针下调氯胺酮滥用大鼠眶额叶皮质c-Fos、TH的表达[J]. 包头医学院学报, 2019, 35(1): 59-60.
[24] 王强, 王渊, 牛文民, 等. 电针不同穴组对功能性腹泻大鼠下丘脑及结肠5-羟色胺和c-fos蛋白表达的影响[J]. 针刺研究, 2019, 44(7): 501-505.
[25] Van Heukelum S, Mars R B, Guthrie M, et al. Where is cingulate cortex? A cross-species view[J]. Trends Neurosci, 2020, 43(5): 285-299.
[26] 李德鹏, 徐群渊, 张进禄, 等. 大鼠PD模型多巴胺能神经元损毁与纹状体相关神经元内c-fos的表达[J]. 首都医科大学学报, 2000, 21(1): 16-19.
[27] Wang Y, Yao L, Gao S S, et al. Inhibition of striatal dopamine D5 receptor attenuates levodopa-induced dyskinesia in a rat model of Parkinson’s disease[J]. Brain Res, 2021, 1754: 147266.
[28] Gomez G, Escande M V, Suarez L M, et al. Changes in dendritic spine density and inhibitory perisomatic connectivity onto medium spiny neurons in L-dopa-induced dyskinesia[J]. Mol Neurobiol, 2019, 56(9): 6261-6275.

电针对帕金森病模型小鼠纹状体背侧的c-Fos表达的选择性调节

Electroacupuncture selectively regulates the expression of c-Fos in the dorsal striatum of Parkinsonian mice

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