CD200R1缺失促进脂多糖诱导的小胶质细胞内吞

doi:10.3969/j.issn.1006-7795.2022.01.015

首都医科大学学报 ›› 2022, Vol. 43 ›› Issue (1): 82-90.doi: 10.3969/j.issn.1006-7795.2022.01.015

CD200R1缺失促进脂多糖诱导的小胶质细胞内吞

杜天舒^1,2, 宫晓丽³, 艾孜儿·艾尼瓦尔^1,2, 张震^1,2, 刘玚^1,2, 王晓民^2,3,4*, 张婷^1,2*

1.首都医科大学基础医学院神经生物学系,北京 100069;
2.首都医科大学教育部神经变性病重点实验室, 北京 100069;
3.首都医科大学基础医学院生理与病理生理学系,北京 100069;
4.北京脑重大疾病研究院,北京 100069

收稿日期:2021-04-30 出版日期:2022-02-21 发布日期:2022-01-27
基金资助:
国家自然科学基金(31971094),国家自然科学基金重点项目(81527901), 国家重点研究与发展计划 (2016YFC1306300),北京市教委科技计划一般项目(KM202010025032)。

CD200R1 deletion promotes LPS-induced endocytosis of microglia

Du Tianshu^1,2, Gong Xiaoli³, Aizier Ainiwaer^1,2, Zhang Zhen^1,2, Liu Yang^1,2, Wang Xiaomin^2,3,4*, Zhang Ting^1,2*

1. Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China;
2. Key Laboratory of Neurodegenerative Disorders of the Ministry of Education, Capital Medical University, Beijing 100069, China;
3. Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China;
4. Beijing Institute for Brain Disorders, Beijing 100069, China

Received:2021-04-30 Online:2022-02-21 Published:2022-01-27
Contact: * E-mail:xmwang@ccmu.edu.cn;zhang.t@ccmu.edu.cn
Supported by:
National Natural Science Foundation of China(31971094,81527901),National Key Research and Development Plan (2016YFC1306300),Scientific Research Common Program of Beijing Municipal Commission of Education (KM202010025032).

摘要/Abstract

摘要： 目的研究CD200R1对细菌脂多糖(lipopolysaccharide,LPS)诱导的小胶质细胞内吞功能的影响。方法应用LPS激活小胶质细胞并通过荧光微球内吞实验观察小胶质细胞内吞水平的变化以及通过RT-qPCR检测CD200R1mRNA水平的表达,并在BV2-SH-SY5Y共培养模型中同样通过荧光微球内吞实验观察LPS诱导的小胶质细胞内吞功能的变化,最后利用CD200R1基因敲除小鼠提取原代培养的小胶质细胞并加LPS处理,通过荧光微球内吞实验观察其内吞功能的变化。结果 LPS激活小胶质细胞,促进了小胶质细胞内吞水平增加以及CD200R1表达下降。在BV2-SH-SY5Y共培养模型中,神经元细胞接触小胶质细胞后抑制LPS诱导的小胶质细胞内吞增加,CD200R1基因敲除的小胶质细胞加入LPS处理后,内吞水平增加更明显。结论 CD200R1基因敲除的小胶质细胞在LPS刺激后内吞水平增加更明显。

关键词: CD200R1, 小胶质细胞, BV2细胞系, SH-SY5Y细胞系, 脂多糖, 内吞

Abstract: Objective To study the effect of CD200R1 on the endocytosis of microglia induced by bacterial lipopolysaccharide (LPS). Methods The changes of microglia endocytosis level was observed with fluorescentmicrobeads endocytosis experiment, and the expression of CD200R1mRNA was detected with RT-qPCR, and also it was observed in the BV2-SH-SY5Y co-culture model. Moreover, the primary microglia was extracted from CD200R1 knockout mice to observe the changes of the endocytic function induced by LPS. Results LPS activated microglia and promoted the increase of endocytosis and the decrease of CD200R1 expression in microglia. In the BV2-SH-SY5Y co-culture model, the neuronal cells contacted microglia to repress the increase of LPS-induced microglia endocytosis. The level of endocytosis increased more significantly in the CD200R1 knockout microglia treated with LPS. Conclusion CD200R1 knockout microglia showed more significant increase in endocytosis after LPS stimulation.

Key words: CD200R1, microglia, BV2 cell line, SH-SY5Y cell line, lipopolysaccharide, endocytosis

中图分类号:

R329.2
R741

杜天舒, 宫晓丽, 艾孜儿·艾尼瓦尔, 张震, 刘玚, 王晓民, 张婷. CD200R1缺失促进脂多糖诱导的小胶质细胞内吞[J]. 首都医科大学学报, 2022, 43(1): 82-90.

Du Tianshu, Gong Xiaoli, Aizier Ainiwaer, Zhang Zhen, Liu Yang, Wang Xiaomin, Zhang Ting. CD200R1 deletion promotes LPS-induced endocytosis of microglia[J]. Journal of Capital Medical University, 2022, 43(1): 82-90.

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CD200R1缺失促进脂多糖诱导的小胶质细胞内吞

CD200R1 deletion promotes LPS-induced endocytosis of microglia

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