Combining Laser Capture Microdissection and Microarray to Analyze the Gene Expression of Rat Dorsal Root Ganglion Sensory Neurons

doi:10.3785/j.issn.1006-7795.2009.04.023

Journal of Capital Medical University ›› 2009, Vol. 30 ›› Issue (4): 516-520.doi: 10.3785/j.issn.1006-7795.2009.04.023

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Combining Laser Capture Microdissection and Microarray to Analyze the Gene Expression of Rat Dorsal Root Ganglion Sensory Neurons

XU Min, JIA Jian-ping, GENG Zhi-wei, SONG Jue-xian, WU You, LI Hao, LIU Lu, LIU Fang-yan

Department of Neurology, Xuanwu Hospital, Capital Medical University

Received:2008-09-08 Revised:1900-01-01 Online:2009-08-21 Published:2009-08-21

Abstract

Abstract: Objective To test the feasibility of applying laser capture microdissection(LCM) combined with microarray technique in studies on expression of sensory neuron genes in dorsal root ganglion(DRG) of rats. Methods Sensory neurons were obtained from frozen sections of rat L₄-L₆ DRGs by using LCM. The quality and yield of total RNA extracted from captured cells were tested in order to determine the impact of frozen-section cutting, staining and capture procedure. Part of the array results was verified by real-time RT-PCR. Results No degradation was detected, and via a single cycle of T7 linear amplification, the yield was sufficient for RT-PCR and microarray analysis. Conclusion Combined with effective RNA protection and linear amplification, LCM can be applied for gene transcriptional analysis of DRG sensory neurons.

Key words: gene expression, laser capture microdissection, dorsal root ganglion

CLC Number:

Q 789

XU Min;JIA Jian-ping;GENG Zhi-wei;SONG Jue-xian;WU You;LI Hao;LIU Lu;LIU Fang-yan. Combining Laser Capture Microdissection and Microarray to Analyze the Gene Expression of Rat Dorsal Root Ganglion Sensory Neurons[J]. Journal of Capital Medical University, 2009, 30(4): 516-520.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3785/j.issn.1006-7795.2009.04.023

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2009/V30/I4/516

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Combining Laser Capture Microdissection and Microarray to Analyze the Gene Expression of Rat Dorsal Root Ganglion Sensory Neurons

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