Activity of α motor neuron of spinal cord modulated by botulinum toxin A

doi:10.3969/j.issn.1006-7795.2013.02.024

Abstract

Abstract:

Objective To study the modulating effect of botulinum toxin A(BTX-A) on the α motor neurons in spinal cord anterior horn. Methods Forty Wistar rats were randomly divided into four groups: group A, group B, group C, and group D. All rats in each group were anesthetized with 10% chloraldurat (0.3 mL/kg). Dorsal root ganglion(DRG) of L₄, L₅, L₆ on the left were exposed following partial laminectomy under the condition of sterility. In Group A and group B, 7 U/kg, 4 U/kg of BTX-A was injected into DRG respectively. In Group C, DRG was injected with the same volume of physiological saline. Group D is the control group. Spinal reflex function and neuroethology were evaluated before and 1, 3, 7, 14 days after BTX-A administration. Results H reflex latency and amplitude of the left hind limb of the rats were longer(P<0.05) and lower(P<0.01) than that of before BTX-A injection in group A. There was also no significant difference of H reflex latency and amplitude in goup B, C, and D when compared before and after BTX-A injection. H reflex latency and amplitude at different time points after BTX-A injection in group A were longer and lower than those of group B, C and D(P<0.05, P<0.01) on 7 days and 14 days after BTX-A injection. There was no significant difference of H reflex latency and amplitude at different time points after BTX-A injection between goup B and C, goup B and D, group C and D(P>0.05). The degree of modified Rivlin loxotic plate test at different time points is lower when compared after and before BTX-A injection in group A(P<0.05). The degree of modified Rivlin loxotic plate test in group A is lower than that of group B, C and D at different time points after BTX-A injection(P<0.05). There was no significant difference of modified Rivlin loxotic plate test degree between group B and C, group B and D,or group C and D(P>0.05) at different time points after BTX-A injection. Conclusion DRG injection of botulinum toxin type A may fundamentally block circuit of spinal and lower excitability of the α motor neurons in spinal cord anterior horn.

Key words: botulinum toxin type A, dorsal root ganglion, &alpha, motor neurons

CLC Number:

R651.3

GAO Baoqin, TIAN Zhaoxia, LIANG Yun, ZHANG Shaodong, YU Yazhen, WANG Yajie, DENG Yaxian, TIAN Yaping, YANG Weili, WANG Guifen. Activity of α motor neuron of spinal cord modulated by botulinum toxin A[J]. Journal of Capital Medical University, 2013, 34(2): 292-297.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2013.02.024

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2013/V34/I2/292

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Activity of α motor neuron of spinal cord modulated by botulinum toxin A

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