Pathological morphology change of respiratory and peripheral immune organ and the change of immune cells

doi:10.3969/j.issn.1006-7795.2015.05.019

Journal of Capital Medical University ›› 2015, Vol. 36 ›› Issue (5): 761-767.doi: 10.3969/j.issn.1006-7795.2015.05.019

Pathological morphology change of respiratory and peripheral immune organ and the change of immune cells

Qi Jing¹, Lyu Fudong², Duan Xuejing³, Wang Jue¹, Wang Daye¹, Xu Xin¹, Bai Yunfei¹, Li Hongjun², Jin Ronghua², Li Ning², Dai Jie¹

1. Department of Pathology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China;
2. Beijing Youan Hospital, Capital Medical University, Beijing 100069, China;
3. Chinese Academy of Medical Sciences, Beijing Union Medical College, State Key Laboratory, Department of Pathology, Fuwai Cardiovascular Disease Hospital, Beijing 100037, China

Received:2015-03-20 Online:2015-10-21 Published:2015-10-20
Contact: 戴洁 E-mail:sydaijie@ccmu.edu.cn
Supported by:
This study was supported by the Capital Medical Science Development Foundation of China (2009-2091).

Abstract

Abstract: Objective To explore the pathology morphological changes of trachea and peripheral immune organ and immune cells of the new influenza A (H1N1) virus severe infection. Methods To collect Collection of 2009 Beijing influenza A H1N1 intensive deaths 8 cases; Pathological changes of these tissue were observed by HE staining; The immune cell types were tested, quantity and distribution characteristics were made by immunohistochemistry. Results The principal pathologic changes of trachea were necrotizing bronchiolitis with peripheral inflammation, diffuse alveolar damage, pulmonary hemorrhage and fibrosis. Macrophages hyperplasia and little lymphocyte infiltration; Viral antigen was found in the epithelium of the epithelium and macrophage; CD68⁺ macrophage count significantly increased in lung, CD20⁺ B lymphocytes, CD3⁺, CD4⁺, CD8⁺ T count, CD56⁺ NK cells occasional,no obvious differences were found compared with the control group (P>0.05). Spleen and lymph nodes were same,focal histocyte hyperplasia, erythrocytophagy were observed, lymphoid tissue atrophy.IHC cell count showed CD68⁺ macrophage significantly increased, CD20⁺ B, CD3⁺, CD4⁺, CD8⁺ T lymphocytes were significantly lower than the control group (P<0.05). NK cells were rarely seen in the spleen and lymph nodes. The ratio of CD4⁺ and CD8⁺ T lymphocytes between experimental group and control group is not significant different. Conclusion The severe influenza A (H1N1) patients, peripheral immune organs showed significant atrophy, specific immune function was abated and the cellular immune response decreased more significantly.

Key words: the new influenza A(H1N1), autopsy, immune organs, immune cells

CLC Number:

Qi Jing, Lyu Fudong, Duan Xuejing, Wang Jue, Wang Daye, Xu Xin, Bai Yunfei, Li Hongjun, Jin Ronghua, Li Ning, Dai Jie. Pathological morphology change of respiratory and peripheral immune organ and the change of immune cells[J]. Journal of Capital Medical University, 2015, 36(5): 761-767.

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https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I5/761

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Pathological morphology change of respiratory and peripheral immune organ and the change of immune cells

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