Effect of PHF8 gene on the growth of transplanted tumor of esophageal squamous-cell carcinoma in nude mice

doi:10.3969/j.issn.1006-7795.2016.01.003

Abstract

Abstract: Objective To establish nude mice models with implanted tumor of esophageal squamous-cell carcinoma cells, and to observe the effects of PHF8 gene on the growth of implanted tumor. Methods Nude mice were injected subcutaneously into their right posterior flank with human TE-1 esophageal squamous-cell carcinoma cells (control group), lentivirus mediated Non-silencing shRNA TE-1 cells (Non-silencing shRNA group), or lentivirus mediated PHF8 shRNA TE-1 cells (PHF8 shRNA group), respectively. The size of implanted tumor was measured at regular intervals and the growth curve of tumor was portrayed. Animals were sacrificed at 4 weeks, and the silencing of PHF8 in tumor tissues was confirmed by real-time PCR and Western blotting.Results The tumorigenic effect on nude mice was weaker and lower in PHF8 shRNA group, as compared to those in Non-silencing shRNA group and control group. The expression of PHF8 mRNA and protein in PHF8 shRNA group was significantly decreased. Conclusion Lentivirus mediated shRNA effectively inhibited the expression of PHF8 in transplanted tumor of nude mice with human esophageal squamous-cell carcinoma cell lines, and the tumorigenic ability was significantly reduced.

Key words: PHD finger protein 8, lentivirus, esophageal squamous cell carcinoma, transplanted tumor in nude mice

CLC Number:

R735.1

Zhu Shengtao, Sun Xiujing, Li Peng, Guo Qingdong, Zhu Shengquan, Zhang Shutian. Effect of PHF8 gene on the growth of transplanted tumor of esophageal squamous-cell carcinoma in nude mice[J]. Journal of Capital Medical University, 2016, 37(1): 12-16.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2016.01.003

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2016/V37/I1/12

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