Effect of DBHS family members on migration and invasion of esophageal squamous cell carcinoma

doi:10.3969/j.issn.1006-7795.2016.01.002

Abstract

Abstract: Objective To investigate the effects of drosophila behavior human splicing(DBHS) family members, including p54nrb, PSF and PSPC1, on regulation of migration and invasion in human esophageal squamous cell carcinoma (ESCC) cell line TE-8.Methods After transfection with p54nrb, PSF and PSPC1 plasmids, the expression of mRNA and protein in human ESCC cell line TE-8 were determined by qRT-PCR and Western blotting, respectively. Wound healing and Matrigel invasion test were used to detect the effects of p54nrb, PSF and PSPC1 overexpression on migration and invasion of the cells, respectively. Results After 48 h of transfection with p54nrb, PSF and PSPC1 plasmids, the expression of relatives p54nrb, PSF and PSPC1 mRNA and protein levels were increased (P<0.05), respectiveIy. The migration rate was measured by wound healing after overexpression of p54nrb, PSF and PSPC1, was promoted significantly (P<0.05). Matrigel invasion of the cells were significantly increased after p54nrb, PSF and PSPC1 plasmids transfection (P<0.05). Conclusion Overexpression of DBHS family members could promote both migration and invasion of ESCC cell line.

Key words: esophageal squamous cell carcinoma, drosophila behavior human splicing(DBHS), p54nrb, PSF, PSPC1, invasion, migration

CLC Number:

R655.4

Cheng Rui, Zhu Shengtao, Guo Shuilong, Li Peng, Guo Qingdong, Zhang Shutian. Effect of DBHS family members on migration and invasion of esophageal squamous cell carcinoma[J]. Journal of Capital Medical University, 2016, 37(1): 6-11.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2016.01.002

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2016/V37/I1/6

References

[1] Tang S, Gao L, Bi Q, et al. SDR9C7 promotes lymph node metastases in patients with esophageal squamous cell carcinoma[J]. PLoS One, 2013, 8(1): e52184.
[2] van Hagen P, Hulshof MCCM, van Lanschot J J B, et al. Preoperative chemoradiotherapy for esophageal or junctional cancer[J]. N Engl J Med, 2012, 366(22): 2074-2084.
[3] Siegel R, Naishadham D, Jemal A. Cancer statistics, 2013[J]. Cancer J Clin, 2013, 63(1): 11-30.
[4] Hanke J H, Landolfi N F, Tucker P W, et al. Identification of murine nuclear proteins that bind to the conserved octamer sequence of the immunoglobulin promoter region[J]. Proc Natl Acad Sci USA, 1988, 85(10): 3560-3564.
[5] Dong B, Horowitz D S, Kobayashi R, et al. Purification and cDNA cloning of hela cell p54nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF and drosophilaNonA/Bj6[J]. Nucleic Acids Res, 1993, 21(17): 4085-4092.
[6] Kuwahara S, Ikei A, Taguchi Y, et al. PSPC1, NONO, and SFPQ are expressed in mouse Sertoli cells and may function as coregulators of androgen receptor-mediated transcription[J]. Biol Reprod, 2006,75(3): 352-359.
[7] Fox A H, Bond C S, Lamond A I. P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner[J]. Mol Biol Cell, 2005,16(11): 5304-5315.
[8] Kameoka S, Duque P, Konarska M M. p54(nrb) associates with the 5' splice site within large transcription/splicing complexes[J]. EMBO J,2004,23(8): 1782-1791.
[9] Kaneko S, Rozenblatt-Rosen O, Meyerson M, et al. The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination[J]. Genes Dev, 2007,21(14): 1779-1789.
[10] Salton M, Lerentha Y, Wang S, et al. Involvement of Matrin 3 and sfpq/nono in the DNA damage response[J]. Cell Cycle, 2010, 9(8): 1568-1576.
[11] Shav-Tal Y, Zipori D. PSF and p54nrb/NonO-multi-functional nuclear proteins[J]. FEBS Lett, 2002, 531(2): 109-114.
[12] Ha K, Takeda Y, Dynan W S, et al. Sequences in PSF/SFPQ mediate radioresistance and recruitment of PSF/SFPQ-containing complexes to DNA damage sites in human cells[J]. DNA Repair: Amst, 2011, 10(3): 252-259.
[13] Schiffner S, Zimara N, Schmid R, et al. P54nrb is a new regulator of progression of malignant melanoma[J]. Carcinogenesis, 2011, 32(8): 1176-1182.
[14] Clark J, Lu Y J, Sidhar S K, et al. Fusion of splicing factor genes PSF and NonO/p54nrb to the TFE3 gene in papillary renal cell carcinoma[J]. Oncogene, 1997, 15(18): 2233-2239.
[15] Tsofack S P, Garand C, Sereduk C, et al. NonO and RALY proteins are required for YB-1 oxaliplatin induced resistance in colon adenocarcinoma cell lines[J]. Mol Cancer, 2011, 10: 145.
[16] Ishiguro H, Uemura H, Fujinami K, et al. 55 kDa nuclearmatrix protein (nmt55) mRNA is expressed in human prostate cancer tissue and is associated with the androgen receptor[J]. Int J Cancer, 2003, 105(1): 26-32.
[17] Zhang C, Zhanga M X, Shena Y H, et al. Role of NonO-histone interaction in TNFα-suppressed prolyl-4-hydroxylase α1[J]. Biochim Biophys Acta, 2008, 1783(8): 1517-1528.
[18] Yamauchi T, Nakamura N, Hiramoto M, et al. Sepantronium bromide (YM155) induces disruption of the ILF3/p54nrb complex, which is required for survivin expression[J]. Biochem Biophys Res Commun, 2012, 425(4): 711-716.
[19] 曹彦坤, 李润霄,田志辉,等. 食管鳞癌单纯放化疗预后及相关因素分析[J]. 中华肿瘤防治杂志,2015,(16):1297-1302.
[20] 胡倩, 姚和瑞. 长链非编码RNA与肿瘤研究现状[J]. 中华肿瘤防治杂志, 2014,21(21):1746-1750.
[21] Barboro P, Rubagotti A, Orecchia P, et al. Differential proteomic analysis of nuclear matrix in muscle-invasive bladder cancer: potential to improve diagnosis and prognosis[J]. Analytical Cellular Pathology,2008,30(1):13-26.
[22] Hodgson M C, Astapova I, Cheng S, et al. The androgen receptor recruits nuclear receptor CoRepressor (N-CoR) in the presence of mifepristone via its N and C termini revealing a novel molecular mechanism for androgen receptor antagonists[J]. J Biol Chem,2005,280(8):6511-6519.
[23] Figueroa A, Kotani H, Toda Y, Mazan-Mamczarz K, et al. Novel roles of hakai in cell proliferation and oncogenesis[J]. Mol Biol Cell, 2009,20(15):3533-3542.