Cell toxicity and cell uptake study of pullulan acetate nanoparticles to BeWo b30 cells

doi:10.3969/j.issn.1006-7795.2016.04.002

Journal of Capital Medical University ›› 2016, Vol. 37 ›› Issue (4): 418-423.doi: 10.3969/j.issn.1006-7795.2016.04.002

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Cell toxicity and cell uptake study of pullulan acetate nanoparticles to BeWo b30 cells

Jiang Ziwen¹, Zhou Zhimin², Tang Hongbo¹, Du bo², Zhang Qiqing², Dai Yinmei¹

1. Department of Gynecology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China;
2. Tianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China

Received:2016-06-15 Online:2016-08-21 Published:2016-07-18
Supported by:
This study was supported by National Natural Science Foundation of China(81301322),Tianjin Research Program of Application Foundation and Advanced Technology(15JCQNJC14400).

Abstract

Abstract: Objective To provide evidence for application in obstetrics field of drugs during pregnancy and the mechanism of pullulan-base nanoparticles across the placental barrier by investigating the cell uptake of pullulan acetate nanoparticles and the cell toxicity of pullulan acetate nanoparticles to BeWo b30 cells. Methods The fluorescein isothiocyanate labelled pullulan acetate nanoparticles (PA-FITC NPs) were prepared by using dialysis method. The methods to investigate the characteristics involved particle size and distribution, zeta potential and morphology of nanoparticles using dynamic light scattering (DLS) and transmission electron microscopy. The growth curve of BeWo b30 and the cell toxicity of different concentration of PA-FITC NPs were studied by cell counting kit-8. The influence of different NPs concentration, incubation time and incubation temperature on cell up-taking were observed in this study.Results The prepared pullulan acetate nanoparticles are solid and spherical in shape. The mean diameter of PA-FITC NPs by DLS were (348.0±114.3) nm and the Zeta potential was (-26.0±5.1) mV. Cell viability in all concentration groups have no significant difference compared with the control group (P>0.05); higher PA-FITC NPs concentration and longer incubation time showed an increased cell uptake; cell uptake was decreased significantly after the temperature was reduced to 4℃. Conclusion The results demonstrated that there was no obvious toxic effect of pullulan acetate nanoparticles on BeWo b30 cells; There is a positive correlation between cell uptake and the concentration, incubation time and environment temperature of PA-FITC NPs.

Key words: pullulan, BeWo b30, nanomedicine, drug delivery in pregnancy

CLC Number:

R318

Jiang Ziwen, Zhou Zhimin, Tang Hongbo, Du bo, Zhang Qiqing, Dai Yinmei. Cell toxicity and cell uptake study of pullulan acetate nanoparticles to BeWo b30 cells[J]. Journal of Capital Medical University, 2016, 37(4): 418-423.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2016.04.002

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2016/V37/I4/418

References

[1] Tang H, Feng X, Zhang T, et al. Stability, pharmacokinetics, biodistributionand safety assessment of folate-conjugated pullulan acetate nanoparticles as cervical cancer targeted drug carriers [J]. J Nanosci Nanotechnol, 2015,15(9):6405-6412.
[2] Zhou Z, Anselmo A C, Mitragotri S. Synthesis of protein-based, rod-shaped particles from spherical templates using layer-by-layer assembly [J]. Adv Mater,2013, 25(19):2723-2727.
[3] Singh R S, Kaur N, Kennedy J F. Pullulan and pullulan derivatives as promising biomolecules for drug and gene targeting [J]. Carbohydr Polym, 2015,123:190-207.
[4] Tang H B, Li L, Chen H. Stability and in vivo evaluation of pullulan acetate as a drug nanocarrier [J]. Drug Deliv, 2010,17(7):552-558.
[5] Wang Y, Chen H, Liu Y, et al. pH-sensitive pullulan-based nanoparticle carrier of methotrexate and combretastatin A4 for the combination therapy against hepatocellular carcinoma [J]. Biomaterials, 2013,34(29):7181-7190.
[6] Zhang C, An T, Wang D, et al. Stepwise pH-responsive nanoparticles containing charge-reversible pullulan-based shells and poly (β-amino ester)/poly(lactic-co-glycolic acid) cores as carriers of anticancer drugs for combination therapy on hepatocellular carcinoma [J]. J Control Release, 2016,226:193-204.
[7] Avery M L, Meek C E, Audus K L. The presence of inducible cytochrome P450 types 1A1 and 1A2 in the BeWo cell line [J]. Placenta, 2003,24(1):45-52.
[8] Bode C J, Jin H, Rytting E, et al. In vitro models for studying trophoblast transcellular transport [J]. Methods Mol Med, 2006,122:225-239.
[9] 唐红波,周志敏,闫素英,等. 纳米递药系统胚胎毒性研究进展 [J]. 药物不良反应杂志, 2015,17(1):44-45.
[10] Fujitani T, Ohyama K, Hirose A, et al. Teratogenicity of multiwall carbon nanotube (MWCNT) in ICR mice [J]. J Toxicol Sci, 2012,37(1):81-89.
[11] Yamashita K, Yoshioka Y, Higashisaka K, et al. Silica and titanium dioxide nanoparticles cause pregnancy complications in mice [J]. Nat Nanotechnol, 2011,6(5):321-328.
[12] Kloet S K, Walczak A P, Louisse J, et al. Translocation of positively and negatively charged polystyrene nanoparticles in an in vitro placental model [J]. Toxicol In Vitro, 2015,29(7):1701-1710.
[13] Gitrowski C, Al-Jubory A R, Handy R D. Uptake of different crystal structures of TiO2 nanoparticles by Caco-2 intestinal cells [J]. Toxicol Lett, 2014,226(3):264-276.
[14] Benfer M, Kissel T. Cellular uptake mechanism and knockdown activity of siRNA-loaded biodegradable DEAPA-PVA-g-PLGA nanoparticles [J]. Eur J Pharm Biopharm, 2012,80(2):247-256.
[15] 黄乾鹏,朱立新,许小亮,等. 免疫金纳米笼子介导热疗诱导人乳腺癌细胞凋亡研究[J]. 中华肿瘤防治杂志,2014,21(15):1129-1133.
[16] 郏亚静,陈红丽,唐红波,等. 普鲁兰基肿瘤靶向纳米粒的体外抑瘤效应和细胞摄取途径研究 [J]. 医学研究生学报, 2015(2):127-130.

Cell toxicity and cell uptake study of pullulan acetate nanoparticles to BeWo b30 cells

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