Evaluation of the inhibitory effect of docetaxel-loaded polymeric micelles on breast cancer metastasis in mice

doi:10.3969/j.issn.1006-7795.2020.02.013

Journal of Capital Medical University ›› 2020, Vol. 41 ›› Issue (2): 225-230.doi: 10.3969/j.issn.1006-7795.2020.02.013

• Basic Research • Previous Articles Next Articles

Evaluation of the inhibitory effect of docetaxel-loaded polymeric micelles on breast cancer metastasis in mice

Wang Aiting^1,2, Yan Dan^1,2, Qi Xianrong³

1. Department of Pharmacy, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China;
2. Beijing Key Laboratory of Bio-characteristic Profiling for Evaluation of Rational Drug Use, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China;
3. School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China

Received:2019-07-10 Online:2020-04-21 Published:2020-04-16
Supported by:
This study was supported by National Great New Drugs Development Project of China （2017ZX09301-040）， Beijing Excellent Talents Training Fund （2017000021469G267）.

Abstract

Abstract: Objective To investigate the inhibitory effect of docetaxel-loaded polymeric micelles on breast cancer metastasis in mice. Methods Two kinds of polymer micelles, DSPE-mPEG₂₀₀₀-Micelles (DM) and D-α-tocopheryl polyethylene glycol 1 000 succinate (TPGS₁₀₀₀)/DSPE-mPEG₂₀₀₀-Micelles (TDM), loading with docetaxel (DTX), were prepared by film formation method. The encapsulation, drug loading, size and zeta potential of micelles were evaluated. A mouse lung metastasis model was established by injecting 4T1/Luc cells into the tail vein. The bioluminescence intensity and nodule number of lung tissues after treatment were evaluated. Results The encapsulation rates of prepared polymeric micelles were more than 85% and the drug loading efficiencies were about 3%. The particle sizes were about 20 nm, and the zeta potentials were about -4 mV. The polymeric micelles containing TPGS₁₀₀₀ were characterized with a higher encapsulation rate and drug loading efficiency, and a smaller particle size. Both DM and TDM groups could reduce the bioluminescence intensity of lung tumors and the number of pulmonary nodules in mice with breast cancer lung metastasis model, whilst TDM showed a stronger effect. Conclusion Docetaxel-loaded polymeric micelles containing TPGS₁₀₀₀ exhibited a stronger inhibitory effect on breast cancer metastasis in mice.

Key words: docetaxel, D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS₁₀₀₀), polymeric micelles, breast cancer metastasis

CLC Number:

R943

Wang Aiting, Yan Dan, Qi Xianrong. Evaluation of the inhibitory effect of docetaxel-loaded polymeric micelles on breast cancer metastasis in mice[J]. Journal of Capital Medical University, 2020, 41(2): 225-230.

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Evaluation of the inhibitory effect of docetaxel-loaded polymeric micelles on breast cancer metastasis in mice

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