Influence of intermittent PTHrP stimulation on apoptosis and cementogenesis in cementoblasts

doi:10.3969/j.issn.1006-7795.2021.03.013

Abstract

Abstract: Objective To investigate the effect of intermittent parathyroid hormone related protein(PTHrP) on apoptosis and the expression of cementum mineralization related proteins and cellular factors in cementoblasts. Methods OCCM-30 cells were stimulated intermittently with PTHrP (1-36) and PTH1R antagonist PTHrP (7-34). The intermittent administration consisted of three cycles, 48 hours/cycle. The cells were divided into control group, PTHrP (1-36) group and PTH1R blocker group. Cell apoptosis was detected by flow cytometry, osteopontin (OPN), collagen (COL-1) and insulin-like growth factor-1 (IGF-1) were detected by real-time PCR and Western blotting. Results Intermittent administration of PTHrP inhibited the apoptosis of cementoblasts and PTH1R antagonist promoted the apoptosis (P<0.05), intermittent PTHrP significantly increased the mRNA and protein expression of OPN, COL-1 and IGF-1 in cementoblasts (P<0.05) compared with control group. PTH1R antagonist inhibited the mRNA and protein expression of OPN, COL-1 and IGF-1 in cementoblasts (P<0.05). Conclusion Our study indicates that intermittent PTHrP could inhibit apoptosis and promote the cementogenesis by interacting with PTH1R in cementoblasts.

Key words: parathyroid hormone related protein (PTHrP), parathyroid hormone type Ⅰ receptor (PTH1R), cementoblast, intermittent stimulation

CLC Number:

R783.5

Li Shengnan, Li Fan, Guan Xiuchen, Bai Yuxing. Influence of intermittent PTHrP stimulation on apoptosis and cementogenesis in cementoblasts[J]. Journal of Capital Medical University, 2021, 42(3): 418-424.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2021.03.013

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2021/V42/I3/418

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