Determination of limonene in volatile oil from citrus peel by gas chromatography

doi:10.3969/j.issn.1006-7795.2021.03.017

Abstract

Abstract: Objective To establish a method for the content determination of limonene in volatile oil from citrus peels. Methods The volatile oil was extracted by steam distillation and the content of limonene was determined by gas chromatography. Gas chromatography conditions were as follows: the capillary column was HP-5(30 m×0.32 mm×0.25 μm)with hydrogen flame ionization detector (FID), and the temperature of inlet and detector were 250 ℃. The temperature program of colum oven started at 70 ℃, holding for 16 minutes, then increased to 250 ℃ at a rate of 30 ℃·min^-1, kept for 1 min. The carrier gas was nitrogen with flow rate of 1 mL·min^-1. Split ration was 100∶1, and injection volume was 1 μL. Results Limonene was linear in the range of 0-17.6 mg·mL^-1(r²=0.999 0). The average recovery was 103.4%(n=9), and relative standard deviation (RSD) was 2.10%(n=9). Conclusion The experiment method has good repeatability, high sensitivity and accuracy, which can be used to determine the content of limonene and control quality in volatile oil from citrus peel.

Key words: gas chromatography, citrus peels, volatile oil, limonene, content determination

CLC Number:

R284

Shao Jianqun, Li Qingyuan, Li Fenghua, Shi Chen, Wang Yuji, Zhang Feng. Determination of limonene in volatile oil from citrus peel by gas chromatography[J]. Journal of Capital Medical University, 2021, 42(3): 443-446.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2021.03.017

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2021/V42/I3/443

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