Joint effects of soy lecithin and soy isoflavone on improving learning and memory impairment and cerebrovascular function in rats

doi:10.3969/j.issn.1006-7795.2021.06.015

Abstract

Abstract: Objective To explore the effects of soy lecithin (SL) combined with soy isoflavone (SIF) on cognitive function and cerebrovascular damage in rats with learning and memory impairment, and to explore the best combined dosage in order to provide scientific evidence of the neuroprotective effects of soybean's active substances and their combined supplementary effects. Methods: In this study, SPF-grade adult male Wistar rats were used. Using the L9(34) orthogonal experimental design, control group, β-amyloid(Aβ) group and nine different dose combinations of SIF+SL were obtained. Methods Such as water maze, cerebrovascular HE staining, enzyme-linked immunosorbent assay (ELISA), and enzymatic kits were used. The rat's learning and memory ability, cerebrovascular pathological damage, the level of oxidative damage, and the redox balance system were tested. Results Compared with the control group, the escape latency of the Aβ model group rats was significantly prolonged (P<0.05), and the morphological changes of cerebrovascular tissue were obvious, and there were increased significant changes in malondialdehyde (MDA) and 3-nitrotyrosine (3-NT) in rats (P<0.05), the ratio of glutathione/oxidized glutathione (GSH/GSSG) decreased significantly (P<0.05). On the contrary, compared with the model group in the SL+SIF intervention group, SL+SIF played a protective role for the model group. Based on the above results, the study found that SL(40 mg·kg^-1·d^-1)+SIF(50 mg·kg^-1·d^-1) is the best combined dose to improve the rat's learning and memory impairment, protect the cerebral blood vessels and regulate the redox balance system. Conclusion SL+SIF can significantly improve Aβ-mediated learning and memory impairment in rats, and its neuroprotective effect may be related to the antioxidant activity of the two to antagonize Aβ-mediated cerebrovascular injury, and their neuroprotective effects of SL(40 mg·kg^-1·d^-1)+SIF(50 mg·kg^-1·d^-1) is the best combined dose for rats.

Key words: soy lecithin, soy isoflavone, combination, learning and memory impairment, β-amyloid peptide

CLC Number:

R459.3

Yu Huiyan, Chi Yafei, Li Hongrui, Wang Xuan, Zhou Xueyang, Xiao Rong, Xi Yuandi. Joint effects of soy lecithin and soy isoflavone on improving learning and memory impairment and cerebrovascular function in rats[J]. Journal of Capital Medical University, 2021, 42(6): 993-999.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2021.06.015

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2021/V42/I6/993

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