Effects of high fat diet with low dose TCDD on hepatic lipid metabolism in female obesity-prone SD rats

doi:10.3969/j.issn.1006-7795.2023.06.019

Abstract

Abstract: Objective To study the effect of combination of high fat diet (HFD) induced obesity and low dose persistent organic pollutants 2,3,7,8-tetrachlorodibenzo-p-dioxin （TCDD) on liver lipid metabolism. Methods By 2×2 factorial design, the effects of combination of HFD and low dose TCDD (10 ng·kg^－1·d^－1) on liver lipid metabolism in obesity-prone (OP) 10-week-old female rats were studied. The four groups of experimental animals were: the group treated with no TCDD in chow diet (Cont+TCDD 0), the group treated with low dose TCDD in chow diet (Cont+TCDD 10), and the group high fat diet without TCDD (HFD+TCDD 0) and high fat diet group treated with low dose TCDD (HFD+TCDD 10). Lipid deposition in liver was observed by oil red O staining. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) in liver were determined by enzyme method. mRNA expression of genes related to lipid metabolism in liver was detected by real-time fluorescence quantitative polymerase chain reaction (RT-qPCR). Results The body weight of rats in Cont+TCDD 0 group, Cont+TCDD 10 group, HFD+TCDD 0 group and HFD+TCDD 10 group were (301.29±18.47) g, (312.16±20.45) g, (358.20±27.11) g and (373.83±26.50) g, respectively (F=7.43, P<0.01), liver oil red O positive area ratios were （1.26±0.16)%,（1.66±0.50)%，（9.06±1.54)%,（32.56±2.66)% (F=11.32, P<0.01), liver SOD activity were (1.54±0.06) U/mg, (1.41±0.15) U/mg, (1.25±0.08) U/mg and (0.76±0.11) U/mg (F=4.31, P<0.05), respectively. Liver GSH-PX activity were Liver GSH-PX activity were (1 511.00±69.30) U/mg, (1 409.00±60.81) U/mg, (1 232.00±65.05) U/mg and (965.00±83.44) U/mg (F=3.91, P<0.05). The interaction analysis of the above indexes suggested that the combination of high fat diet and low dose TCDD resulted in synergistic increases in the body weight (F=6.33, P<0.05) and liver fat accumulation (F=12.51, P<0.01) of obese-prone female SD rats, and the activities of antioxidant enzymes such as SOD in liver (F=4.15, P<0.05) and GSH-PX enzyme activity (F=3.97, P<0.05) were decreased synergistically. Analysis on mRNA expression levels of 12 genes related to lipid metabolism in liver showed that high fat diet combined with low dose TCDD increased adipose lipase (Atgl) (F=3.69, P<0.05), mRNA expression of cluster of differentiation 36 (CD36) (F=5.58, P<0.01), inhibited the mRNA expression of hormone sensitive lipase (Hsl) (F=4.01, P<0.05), and there was no significant difference in the expression of other 9 genes (P>0.05). Conclusion The combination of high fat diet and low dose TCDD can synergistically increase the abnormal liver lipid metabolism in female obesity-prone female SD rats.

Key words: obesity, 2,3,7,8-tetrachlorodibenzo-p-dioxin, diet high fat, oxidative stress

CLC Number:

Liu Yue, Zhu Dan, Hong Yujing, Sun Wenxing, Xu Guangfei. Effects of high fat diet with low dose TCDD on hepatic lipid metabolism in female obesity-prone SD rats[J]. Journal of Capital Medical University, 2023, 44(6): 1029-1035.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2023.06.019

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2023/V44/I6/1029

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