Journal of Capital Medical University ›› 2015, Vol. 36 ›› Issue (2): 291-298.doi: 10.3969/j.issn.1006-7795.2015.04.023

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Surgery management of implantable left ventricular assist device in sheep model

Wu Guanghui1, Lin Changyan1, Hou Xiaotong2, Li Haiyang3, Chen Chen4, Liu Xiujian1, Xu Chuangye1, Wang Jing1, Yang Peng4, Qu Wenbo4   

  1. 1. Biomedical Engineering Laboratory, Beijing Institute of Heart Lung and Blood Vessel Disease, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China;
    2. Cardiac Surgery Ward 5, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China;
    3. Cardiac Surgery Critical Care Center, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China;
    4. Chinaheart Biomedical Incorporation, Suzhou 215125, Jiangsu Province, China
  • Received:2014-10-20 Published:2015-04-16
  • Supported by:

    This study was supported by National High Technology Research and Development Program of China(2012AA041265), Capital Health Research Fund(2011-1005-02).

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Abstract:

Objective We performed this study to explore particular management requirement on sheep experimental implantation of left ventricular assist device(LVAD). Methods In accordance with the Beijing Laboratory Animal Management Regulations, the criteria of American Association for Laboratory Animal Science(AALAS) and the experiences of the other in vivo animal experiments of the LVAD, we implanted the LVAD in the ovine model. The LVADs were implanted in healthy sheep on beating heart. Considering with the characteristics of the animals and in accordance with the requirements of the LVAD, the managements were formulated on aspects of the perioperative, the perioperative and the postoperative. Results In total 6 sheep survived the surgical procedure, one case was terminated because of lung infection 3 days after the surgery, one case was terminated because of the inflow cannula obstruction associated with a thrombus and cellular fibrous tissue growths at the LV apex 25 days after the surgery, and the remaining four cases survived in good condition after implantation and reached the 30 d expectations. Conclusion Careful preparation before the surgery, development of strict surgical procedure, rigorous early postoperative care and a reasonable choice of anticoagulant strategies can effectively improve the success rate of implantable experiments.

Key words: left ventricular assist device, sheep, experimental animal, surgical management

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