Construction and expressional identification of human hepatic cell stably transfected with HSS

doi:10.3969/j.issn.1006-7795.2015.06.016

Abstract

Abstract: Objective To construct a hepatic cell stably transfected with HSS gene, and to identify its biological characteristics. Methods Flag-HSS-pcDNA3.0 was transfected into BEL-7402 cell line via FuGENE HD and G418 selection. The stably transfected cell line was identified by immunofluorescence, real-time PCR, and Western blotting. Results Immunofluorescence demonstrate the expression of HSS in the stably transfected cell line and the mitochondrial location of HSS. The expression of mRNA and protein levels were significantly increased in the hepatic cell line stably transfected with HSS gene compared with the wild type cell line. Conclusion A new hepatic cell lines transfected with HSS is successfully established, and it will provide the experiment tools and research base to in-depth study of the physiological functions of HSS.

Key words: hepatic stimulator substance, hepatic cell, transfection

CLC Number:

Q503

Wu Yuan, Zhang Jing, Dong Lingyue, An Wei. Construction and expressional identification of human hepatic cell stably transfected with HSS[J]. Journal of Capital Medical University, 2015, 36(6): 924-928.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.06.016

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I6/924

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