Expression of CXCR4 and NDRG1 and its association with invasion and metastasis in prostate cancer

doi:10.3969/j.issn.1006-7795.2016.03.014

Abstract

Abstract: Objective To investigate the expression and clinical significance of CXCR4 and N-myc downstream regulated gene 1 (NDRG1) in prostate cancer (PCa), and explore the relation between the protein expression and metastasis in PCa. Methods The expression of CXCR4 was detected by immunohistochemistry in 46 cases of PCa, 15 cases of benign prostatic hyperplasia (BPH) and 10 cases of normal prostate tissues (NP). The relation between the expression and clinicopathological changes was analyzed statistically. CXCR4 and phosphorylated NDRG1 (pNDRG1) were examined by Western blotting in prostate cancer cells. Results The expression level of CXCR4 in PCa was significantly higher than those in BPH and NP. The over-expression rate of CXCR4 in PCa was 65.2%. The expression of CXCR4 protein was not significantly associated with pathological grade and Gleason score (P=0.081). However, the increased expression of CXCR4 protein was significantly associated with the presence of metastasis of PCa (P=0.002). By Western blotting analysis, the expression of CXCR4 protein was stronger in PC3 and DU145 than in PrEC and LNCap cell lines. However, the expression of pNDRG1 decreased in the prostate cancer cells with high metastasis potential. Conclusion The expressions of CXCR4 and pNDRG1 are closely related to metastasis of PCa. CXCR4 and pNDRG1 have significant clinical implications for diagnosis and treatment of PCa.

Key words: prostate cancer, chemokine receptor 4 (CXCR4), N-myc downstream regulated gene 1 (NDRG1), metastasis

CLC Number:

R737.25

Ping Hao, Niu Yinong, Yang Feiya, Wang Mingshuai, Xing Nianzeng. Expression of CXCR4 and NDRG1 and its association with invasion and metastasis in prostate cancer[J]. Journal of Capital Medical University, 2016, 37(3): 327-330.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2016.03.014

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2016/V37/I3/327

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