Detection of the interaction between the peptide and β1-adrenergic receptor autoantibodies using surface plasmon resonance

doi:10.3969/j.issn.1006-7795.2016.06.004

Abstract

Abstract: Objective To investigate the interaction between the peptide which mimic the structure of the second extracellular loop of the β₁-adrenergic receptor (β₁-AR-EC_Ⅱ) and β₁-adrenergic receptor autoantibodies (β₁-AA). Methods Peptide was synthesized according to the amino acid sequence of human β₁-AR-EC_Ⅱ; a hybridoma fusion method was used to obtain anti-β₁-AR-EC_Ⅱ monoclonal antibody; the interaction between the peptide and β₁-AA was detected by surface plasmon resonance (SPR); and the beating frequency experiment of neonatal rat cardiomyocytes was explored to test the neutralization of the peptide to β₁-AA. Results β₁-AA enhanced the beating frequency of neonatal rat cardiomyocytes (P<0.05), indicating that the β₁-AA we obtained has biological activity; SPR results showed that there was a moderate binding affinity (K_D) of 4.44 μmol/L between the synthetic peptide and β₁-AA. Conclusion There was interaction between the synthesized peptide and β₁-adrenergic receptor autoantibodies.

Key words: β₁-adrenergic receptor autoantibodies(β₁-AA), 26 peptide, surface plasmon resonance

CLC Number:

R971

Dong Yu, Lyu Tingting, Xu Wenli, Bai Yan, Wu Ye, Liu Huirong, Wang Wen. Detection of the interaction between the peptide and β₁-adrenergic receptor autoantibodies using surface plasmon resonance[J]. Journal of Capital Medical University, 2016, 37(6): 736-739.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2016.06.004

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2016/V37/I6/736

References

[1] Fiore G, Suppress P, Triggiani V, et al. Neuroimmune activation in chronic heart failure[J]. Enocr Metab Immun Disord Drug Targets, 2013,13(1): 68-75.
[2] Wang L, Lu K, Hao H, et al. Decreased autophagy in rat heart induced by anti-β₁-adrenergic receptor autoantibodies contributes to the decline in mitochondrial membrane potential[J]. PLoS One, 2013,8(11):e81296.
[3] Du Y, Yan L, Wang J, et al. β₁-Adrenoceptor autoantibodies from DCM patients enhance the proliferation of T Lymphocytes through the β₁-AR/cAMP/PKA and p38 MAPK Pathways[J]. PLoS One, 2012,7(12): e52911.
[4] Du Y, Yan L, Du H, et al. β₁-adrenergic receptor autoantibodies from heart failure patients enhanced TNF-α secretion in RAW264.7 macrophages in a largely PKA-dependent fashion[J]. J Cell Biochem, 2012, 113(10): 3218-3228.
[5] Zuo L, Du Y, Ma J, et al. Pro-arrhythmic action of autoantibodies against the second extracellular loop of β₁-adrenoceptor and its underlying molecular mechanisms[J]. Int J Cardiol, 2015,198: 251-258.
[6] Zhao Y, Huang H, Du Y, et al.β₁-Adrenoceptor autoantibodies affect action potential duration and delayed rectifier potassium currents in guinea pigs[J]. Cardiovasc Toxicol, 2015,15(1):1-9.
[7] Nitsche A, Kurth A, Dunkhorst A, et al. One-step selection of vaccinia virus-binding DNA aptamers by MonoLEX[J].BMC Biotechnol, 2007,7: 48.
[8] Haberland A, Wallukat G, Dahmen C, et al. Aptamer neutralization of beta1-adrenoceptor autoantibodies isolated from patients with cardiomyopathies[J]. Circ Res, 2011,109(9): 986-992.
[9] Haberland A, Wallukat G, Berg S, et al. Neutralization of pathogenic beta1-receptor autoantibodies by aptamers in vivo: the first successful proof of principle in spontaneously hypertensive rats[J]. Mol Cell Biochem, 2014,393(1-2): 177-180.
[10] Jahns R, Schlipp A, Boivin V, et al. Targeting receptor antibodies in immune cardiomyopathy[J]. Semin Thromb Hemost, 2010,36(2):212-218.
[11] Pei J, Li N, Chen J, et al. The predictive values of beta1-adrenergic and M₂ muscarinic receptor autoantibodies for sudden cardiac deathin patients with chronic heart failure[J]. Eur J Heart Fail, 2012,14(8): 887-894.
[12] Li H, Zhang L, Huang B, et al.A peptidomimetic inhibitor suppresses the inducibility of β₁-adrenergic autoantibody-mediated cardiac arrhythmias in the rabbit[J]. J Interv Card Electrophysiol, 2015,44(3):205-212.
[13] Jahns R, Boivin V, Hein L, et al. A new cyclic receptor peptide prevents development of heart dilatation and failure induced by antibodies activating cardiac beta1-adrenergic receptors[J]. Circulation, 2005,112(17): U53-U53.
[14] Munch G, Boivin-Jahns V, Holthoff HP, et al. Administration of the cyclic peptide COR-1 in humans (phase I study): ex vivo measurements of anti-beta1-adrenergic receptor antibody neutralization and of immune parameters[J]. Eur J Heart Fail, 2012,14(11):1230-1239.
[15] 陈丽华, 苏忠民, 王荣顺. 用表面等离子共振仪进行生物分子相互作用研究的评述[J]. 分子科学学报, 2004,20(4):44-49.
[16] 吴世康, 汪鹏飞. 表面等离子共振(SPR)-一种新型化学检测方法的原理[J].影像科学与光化学, 2008,26(2):157-168.
[17] 徐江敏,张丽丽,李桂芬. 利用表面等离子体共振仪检测黄瓜花叶病毒[J]. 生物技术通讯, 2013,24(6):847-849.

Detection of the interaction between the peptide and β₁-adrenergic receptor autoantibodies using surface plasmon resonance

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