Harnessing the power of evolution:directed evolution of enzymes and binding protein & Phage display-Introduction of Nobel prize in Chemistry 2018

doi:10.3969/j.issn.1006-7795.2018.05.028

Abstract

Abstract: The power of evolution is revealed through the diversity of life. The Nobel Prize in Chemistry 2018 was awarded to Frances H. Arnold, George P. Smith and Sir Gregory P. Winter for the way they have taken control of evolution and used it for the greatest benefit to humankind. Enzymes developed through directed evolution are now used to produce biofuels and pharmaceuticals, among other things. Antibodies evolved using a method called phage display can combat autoimmune diseases, and in some cases, cure metastatic cancer. This article overviewed their research discoveries with the highlighted significance.

Key words: the 2018 Nobel Prize in Chemistry, enzymes, directed evolution, phage display, antibodies

Cao Lixue. Harnessing the power of evolution:directed evolution of enzymes and binding protein & Phage display-Introduction of Nobel prize in Chemistry 2018[J]. Journal of Capital Medical University, 2018, 39(5): 770-777.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2018.05.028

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2018/V39/I5/770

References

[1] Press release:The Nobel Prize in Chemistry 2018[EB/OL].(2018-10-03) https://www.nobelprize.org/prizes/chemistry/2018/press-release/.
[2] Eigen M, Gardiner W. Evolutionary molecular engineering based on RNA replication[J]. Pure Appl Chem, 1984,56(8):967-978.
[3] Chen K, Arnold F H. Tuning the activity of an enzyme for unusual environments:sequential random mutagenesis of subtilisin E for catalysis in dimethylformamide[J]. Proc Natl Acad Sci U S A, 1993, 90(12):5618-5622.
[4] Arnold F H. Protein engineering for unusual environments[J]. Curr Opin Biotechnal, 1993, 4(4):450-455.
[5] You L, Arnold F H. Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide[J]. Protein Eng, 1996, 9(1):77-83.
[6] Moore J C, Arnold F H. Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents[J]. Nat Biotechnol, 1996, 14(4):458-467.
[7] Stemmer W P. Rapid evolution of a protein in vitro by DNA shuffling[J]. Nature, 1994, 370(6488):389-391.
[8] Stemmer W P. DNA shuffling by random fragmentation and reassembly:in vitro recombination for molecular evolution[J]. Proc Natl Acad Sci U S A, 1994, 91(22):10747-10751.
[9] Clackson T, Hoogenboom H R, Griffiths A D, et al. Making antibody fragments using phage display libraries[J]. Nature, 1991, 352(6336):624-628.
[10] Marks J, Griffiths A D, Malmqvist M, et al. By-passing immunization:building high affinity human antibodies by chain shuffling[J]. Biotechnology (N Y), 1992, 10(7):779-783.
[11] Collet T A, Roben P, O'kennedy R, et al. A binary plasmid system for shuffling combinatorial antibody libraries[J]. Proc Natl Acad Sci USA, 1992, 89(21):10026-10030.
[12] Smith G P. Filamentous fusion phage:novel expression vectors that display cloned antigens on the virion surface[J]. Science, 1985, 228(4705):1315-1317.
[13] Parmley S F, Smith G P. Antibody-selectable filamentous fd phage vectors:affinity purification of target genes[J]. Gene, 1988. 73(2):305-318.
[14] Devlin J J, Panganiban L C, Devlin P E. Random peptide libraries:a source of specific protein binding molecules[J]. Science, 1990, 249(4967):404-406.
[15] Scott J K, Smith G P. Searching for peptide ligands with an epitope library[J]. Science, 1990, 249(4967):386-390.
[16] McCafferty J, Griffiths A D, Winter G. Phage antibodies:filamentous phage displaying antibody variable domains[J]. Nature, 1990, 348(6301):552-554.
[17] Huston J S, Levinson D, Mudgett-Hunter M, et al. Protein engineering of antibody binding sites:recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli[J]. Proc Natl Acad Sci U S A, 1988, 85(16):5879-5883.
[18] Kang A S, Barbas C F, Janda K D, et al. Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces[J]. Proc Natl Acad Sci U S A, 1991, 88(10):4363-4366.