Correlation study between thyrotropin receptor antibodies (TRAb) and intestinal flora and short-chain fatty acids in patients with Graves disease

doi:10.3969/j.issn.1006-7795.2022.06.018

Abstract

Abstract: Objective To investigate the differences in intestinal microbiota and short-chain fatty acids between patients suffering from Graves disease (GD) with normal thyroid function and healthy controls, and to analyze the correlation between thyrotropin receptor auto-antibodies (TRAb) and different intestinal microbiota. Methods Totally 30 Graves patients with normal thyroid function (GD group) and 32 healthy controls (Control) were enrolled in our study.Feces were collected, and 16S rRNA sequencing was used to analyze the structure of intestinal bacteria, and gas chromatography-mass spectrometry (GC-MS) was used to determine the content of short-chain fatty acids, the relationship between TRAb and intestinal microbiota was analyzed. Results Compared with the control group, α-diversity indexes of intestinal flora in GD patients were significantly decreased statistically.At the family level, the abundance of Bacteroidaceae and Ruminococcaceae decreased in GD patients, while the abundance of Prevotellaceae and Erysipelotrichaceae increased.At the species level, Eubacterium _hallii, Ruminococcus and Eubacterium_ventriosum were increased in GD patients, whileRuminococcus-bicirculans and Bacteroide_vulgatus decreased, and the differences were statistically significant.LEfSe analysis showed that Prevotellaceae (f_prevotellaceae and g_unidentified_prevotellaceae) and anaerobic bacteria (s_Prevotella copri) were enriched in GD group.The levels of propionic acid and butyric acid in GD patients were significantly decreased.The level of propionic acid was positively correlated with the abundance of Ruminococcaceae. Our analysis showed that serum TRAb antibody was inversely correlated with Ruminococcus abundance. Conclusion Even if the thyroid function of patients with Graves disease was normal, the intestinal flora and levels of propionic acid in patients were still significantly changed, and this change may be related to the serum TRAb antibody.

Key words: Graves disease, thyrotropin receptor auto-antibodies (TRAb), intestinal bacteria, short-chain fatty acids, 16S rRNA

CLC Number:

R581

Wang Hua, Shi Tingting, Xin Zhong, Hua Lin, Li Cong, Cao Qiumei. Correlation study between thyrotropin receptor antibodies (TRAb) and intestinal flora and short-chain fatty acids in patients with Graves disease[J]. Journal of Capital Medical University, 2022, 43(6): 931-939.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2022.06.018

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2022/V43/I6/931

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