Abstract: Objective To find out more effective vaccine candidate antigen against the Trichinella spiralis,and to construct the adult cDNA plasmid library of T.spiralis.Methods The cDNA fragments were amplified using adult cDNA plasmid library as the template by PCR and then the fragments digested with BamHⅠand HindⅢ to the size of 500～1500 bp were cloned into a eukaryotic expression vector pcDNA3.1.The inserted DNA fragments in the recombinants were identified by digestion with restriction enzyme.Results The partial adult cDNA plasmid library of T.spiralis,with 10⁵ transformants,was constructed.Most of the recombinants contained the insert DNA fragments.Conclusion A partial adult cDNA plasmid library of T.spiralis is constructed successfully.

Key words: Trichinella spiralis, cDNA expression plasmid library, construction