不同性别大鼠局灶性脑缺血脑血流及梗死体积的变化

doi:10.3969/j.issn.1006-7795.2013.01.012

首都医科大学学报 ›› 2013, Vol. 34 ›› Issue (1): 64-68.doi: 10.3969/j.issn.1006-7795.2013.01.012

• 神经系统疾病的基础研究 • 上一篇下一篇

不同性别大鼠局灶性脑缺血脑血流及梗死体积的变化

闫峰, 刘向荣, 赵海苹, 王荣亮, 吉训明, 赵咏梅, 陶真, 罗玉敏

首都医科大学宣武医院脑血管病研究室北京市老年病医疗研究中心, 北京 100053

收稿日期:2012-10-18 出版日期:2013-02-21 发布日期:2013-02-25
通讯作者: 罗玉敏 E-mail:yumin111@ccmu.edu.cn
基金资助:
国家自然科学基金面上项目(81071058, 30770743)。

Changes in cerebral blood flow and infarct volume in different sexes of rats after focal brain ischemia

YAN Feng, LIU Xiangrong, ZHAO Haiping, WANG Rongliang, JI Xunming, ZHAO Yongmei, TAO Zhen, LUO Yumin

Cerebrovascular Diseases Research Institute, Xuanwu Hospital, Capital Medical University, Beijing Geriatric Medical Research Center, Beijing 100053, China

Received:2012-10-18 Online:2013-02-21 Published:2013-02-25
Supported by:
This study was supported by National Natural Science Foundation of China (81071058, 30770743).

摘要/Abstract

摘要：

目的探讨正常生理条件下性别因素对大鼠局灶性脑缺血模型脑梗死体积及梗死过程中脑血流变化的影响。方法健康雌、雄(sprague dawley, SD)大鼠(280~310g)各12只,使用大脑中动脉阻塞(middle cerebral artery occlusion, MCAO)线栓模型,大脑中动脉阻塞2 h,再灌注24 h后处死。(1)手术中全程监测大脑中动脉灌注区域脑血流变化,记录梗死前脑血流的基础值,插栓后脑血流的下降值及拔栓后脑血流的恢复值,并比较雌雄2组之间的差异。(2)处死大鼠后进行大脑切片,通过2,3,5-氯化三苯四氮唑(2,3,5-triphenyl tetrazolium,TTC)染色测量并比较雌雄2组大鼠脑梗死体积。结果 (1)脑血流监测基础值雌性为(154±20)PU,雄性为(212±24)PU,雌性显著低于雄性(P<0.01);线栓插入后,雌性为(48±3)PU,雄性为(78±21)PU,雌性显著低于雄性(P<0.05),下降百分比雌性为(68±4)%,雄性为(63±12)%,差异无统计学意义(P>0.05);拔栓后脑血流雌性为(159±5)PU,雄性为(196±36)PU,恢复百分比雌性为(104±11)%,雄性为(91±14)%,差异无统计学意义(P>0.05)。(2)TTC染色发现雌性大鼠脑梗死体积为(28.4±3.3)%,雄性为(40.0±3.1)%,雌性明显小于雄性,差异有统计学意义(P<0.05)。结论正常生理条件下,雌性大鼠脑血流基础值显著低于雄性,单侧MCAO缺血再灌过程中,雌雄2组脑血流和各自基础值相比,下降和恢复的程度差异无统计学意义。因此推测雌性大鼠脑血流基础值低水平状态可能提高其对缺血再灌注损伤的耐受性,最终导致在病死率及脑梗死统计中雌性大鼠都显著低于雄性大鼠。

关键词: 大鼠, 性别, 大脑中动脉阻塞, 脑血流

Abstract:

Objective To study the infarct volumes and the cortical blood flow of the rat model of middle cerebral artery occlusion (MCAO) in different sexes.Methods Twenty-four healthy sprague dawley (SD) rats, including 12 males and 12 females, weighing 280-310 g were studied. The rats were subjected to unilateral middle cerebral artery occlusion (MCAO) for 2 hours and sacrificed after 24 hours reperfusion. The cortical blood flow was continuously monitored by laser Doppler flowmetry (LDF). The base line value, the decreasing amplitude after occlusion and the recovering amplitude after reperfusion of the local blood flow were recorded. And the data of the two groups were compared. Brain slices were obtained at the time of ischemia for 2 hours and reperfusion for 24 hours. The volumes of infarction in the brain slices were determined by triphenyltetrazolium chloride (TTC) staining.Results The average baseline value of cortical blood flow of the females and males were (154±20)PU and (212±24)PU separately. The value of the female was significantly lower than that of the male (P<0.01). The baseline value remarkably decreased to (48±3)PU and (78±21)PU separately after occlusion (P<0.05). The decline in the percentage of females was (68±4)%, and that of males was (63±12)%. There was no significant difference in decreasing amplitude of the blood flow between the different sexes(P>0.05). In the reperfusion period, the average local blood flow rapidly recovered to (159±5)PU and (196±36)PU separately. Recovery percentage of females was(104±11)%, and that of the males was(91±14)%. There was no significant difference in recovering amplitude of the blood flow between the different sexes(P>0.05). The infarct volumes of the female group measured by TTC were significantly smaller than that of the male (P<0.05).Conclusion A steady model of middle cerebral artery occlusion in different sexes of rats was established, and it was found that under the normal physical conditions the the average baseline value of cortical blood flow of the females are significantly lower than that of the males. There are no significant differences in both the decreasing amplitude after occlusion and the recovering amplitude after reperfusion between females and males. However the recovery of the blood flow of the females was better than that of the males. Therefore it is supposed that low-level state of CBF may contribute to the tolerance of female rats to the ischemia-reperfusion injury, and at last improved the neurological functions and decreased the infarct volumes.

Key words: rats, sex, middle cerebral artery occlusion, cerebral blood flow

中图分类号:

R743.31

闫峰, 刘向荣, 赵海苹, 王荣亮, 吉训明, 赵咏梅, 陶真, 罗玉敏. 不同性别大鼠局灶性脑缺血脑血流及梗死体积的变化[J]. 首都医科大学学报, 2013, 34(1): 64-68.

YAN Feng, LIU Xiangrong, ZHAO Haiping, WANG Rongliang, JI Xunming, ZHAO Yongmei, TAO Zhen, LUO Yumin. Changes in cerebral blood flow and infarct volume in different sexes of rats after focal brain ischemia[J]. Journal of Capital Medical University, 2013, 34(1): 64-68.

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不同性别大鼠局灶性脑缺血脑血流及梗死体积的变化

Changes in cerebral blood flow and infarct volume in different sexes of rats after focal brain ischemia

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