首都医科大学学报 ›› 1989, Vol. 10 ›› Issue (2): 101-105.

• 论著 • 上一篇    下一篇

冰冻保存肾上腺髓质组织适宜条件的研究

张国风1, 王红1, 王元身1, 鲁强1, 焦守恕1, 王卫2, 李楠2, 蔡青2   

  1. 1. 首都医学院北京市神经科学研究所;2. 首都医学院解剖学教研室
  • 收稿日期:1988-11-28 修回日期:1900-01-01 出版日期:1989-04-15 发布日期:1989-04-15

Studies on Suitable Factors for Freezing and Storage fo Adrenal Medulla Tissue

Zhang Guofeng1, Wang Hong1, Wang Yuanshen1   

  1. Beijing Institute of Neuroscience, Capital Institute of Medicine
  • Received:1988-11-28 Revised:1900-01-01 Online:1989-04-15 Published:1989-04-15

摘要: 研究了冷冻溶液种类、冰冻速率、组织块体积、保护剂种类、储存时间及从动物死亡至冰冻储存的时间间隔对冰冻后肾上腺髓质组织存活的影响。证实冰冻速率、冷冻溶液和保护剂种类与冰冻储存的肾上腺髓质组织的存活紧密相关。用羊水为冷冻溶液、DMSO作保护剂,以1~2C/min的速率冰冻可获得最好结果。

关键词: 冰冻, 肾上腺髓质, 储存, 保护剂, 培养基

Abstract: The relationships between the survival of adrenal medulla tissue after freezing and some factors were investigated. The factorsin clude freezing mediums, freezing rates, the volumes of tissue, protective agents, storing Periods and the permissible intervial from animal death to freezing. It is proved that the freezing rates, freezing mediums and protective agents are closely related with the survival of frozen-stored adrenal medulla tissue. The best result can be obtained by using amniotic fluid as freezing medium, and DMSO as protective agent for freezing, with the freezing rate of-1~-2℃per minute. This study provides the reference data for the freezing and storage of adrenal medulla tissue.

Key words: freezing, adrenal medulla, storage, protective agent, medium