AD7C-NTP在老年痴呆患者及不同种属动物脑内的存在和意义

首都医科大学学报 ›› 2008, Vol. 29 ›› Issue (2): 158-161.

AD7C-NTP在老年痴呆患者及不同种属动物脑内的存在和意义

张炙萍, 姬志娟, 赵志炜, 王宏娟, 盛树力, 王蓉

首都医科大学宣武医院中心实验室

收稿日期:2007-09-11 修回日期:1900-01-01 出版日期:2008-04-24 发布日期:2008-04-24
通讯作者: 王蓉

Existence and Significance of AD7C-NTP in the Brain of Patients with Alzheimer's Disease and in Different Species of Animals

Zhang Zhiping, Ji Zhijuan, Zhao Zhiwei, Wang Hongjuan, Sheng Shuli, Wang Rong

Expariment Center, Xuanwu Hospital, Capital Medical University

Received:2007-09-11 Revised:1900-01-01 Online:2008-04-24 Published:2008-04-24

摘要/Abstract

摘要： 目的研究Alzheimer相关的神经丝蛋白(Alzheimer-associated neuronal thread protein,AD7C-NTP)在老年痴呆(Alzheimer's disease,AD)患者脑内、猪脑和小鼠脑内的表达,初步确定一种AD7C-NTP蛋白含量丰富的组织来源.方法采用免疫组织化学染色方法观察猪脑、小鼠脑内AD7C NTP的分布,采用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)和蛋白印迹(Western blot)的方法检测AD患者脑内、猪脑、小鼠脑内AD7C-NTP的表达水平.结果免疫组化结果显示,猪大脑皮质、小鼠海马内有AD7C-NTP免疫反应阳性神经元分布;Western blot的结果显示,以AD7C-NTP C端抗体作为检测抗体时,AD患者脑和猪脑内可见相对分子质量为41 000 bp的免疫反应阳性条带,小鼠脑内未见免疫反应阳性条带.结论 AD患者脑内、猪脑内存在AD7C-NTP,可作为提取AD7C-NTP蛋白纯品的来源之一,为研制AD7C-NTP诊断试剂盒提供实验材料.

关键词: Alzheimer相关的神经丝蛋白, 老年痴呆

Abstract: Objective To investigate the expression of Alzheimer-associated neuronal thread protein(AD7C-NTP) in the brain of patients with Alzheimer's disease(AD), as well as in swine and mice, so as to determine the source of tissues rich in AD7C-NTP. Methods The distribution of AD7C-NTP in the brain of pig and mouse was investigated with immunohistochemical methods. SDS-PAGE and Western blot were applied to detect the expression of AD7C-NTP in the brain of patients with AD as well as in pigs and mice. Results The results of immunohistochemistry indicated that AD7C-NTP immunoreactive positive neurons were observed in the cerebral cortices of pigs and hippocampi of mice. The results of Western blot indicated that a band of about 41 000 bp was detected with the antibody of AD7C-NTP against C-terminal in the brain of patients with AD and in pigs. Conclusion The existence of AD7C-NTP in the brain of patients with AD is confirmed; AD7C-NTP is also found in the brain of swine, so that it can be considered as a source for extraction of purified AD7C-NTP protein, which can be used as the experimental material for AD7C-NTP diagnostic kit.

Key words: Alzheimer-associated neuronal thread protein, Alzheimer's disease

中图分类号:

R329.2

张炙萍;姬志娟;赵志炜;王宏娟;盛树力;王蓉. AD7C-NTP在老年痴呆患者及不同种属动物脑内的存在和意义[J]. 首都医科大学学报, 2008, 29(2): 158-161.

Zhang Zhiping;Ji Zhijuan;Zhao Zhiwei;Wang Hongjuan;Sheng Shuli;Wang Rong. Existence and Significance of AD7C-NTP in the Brain of Patients with Alzheimer's Disease and in Different Species of Animals[J]. Journal of Capital Medical University, 2008, 29(2): 158-161.

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