超黄金治疗期的缺血性中风大鼠模型、治疗模式及疗效表述

doi:10.3969/j.issn.1006-7795.2014.01.023

首都医科大学学报 ›› 2014, Vol. 35 ›› Issue (1): 108-112.doi: 10.3969/j.issn.1006-7795.2014.01.023

超黄金治疗期的缺血性中风大鼠模型、治疗模式及疗效表述

蒋雪云¹, 张颖洁², 赵明²

1. 首都医科大学医学实验与测试中心, 北京 100069;
2. 首都医科大学化学生物学与药学院, 北京 100069

收稿日期:2013-06-28 出版日期:2014-02-21 发布日期:2014-02-21
通讯作者: 赵明 E-mail:maozhao@126.com
基金资助:
北京市自然科学基金（7122023）。

Long-term model, administration pattern and efficacy description of rats in acute ischemic stroke condition

Jiang Xueyun¹, Zhang Yingjie², Zhao Ming²

1. Medical Experiment and Test Center, Capital Medical University, Beijing 100069, China;
2. College of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, China

Received:2013-06-28 Online:2014-02-21 Published:2014-02-21
Supported by:
This study was supported by Natural Science Foundation of Beijing (7122023).

摘要/Abstract

摘要：

目的研究长时缺血性中风大鼠模型及治疗有效性和出血不良反应。方法连续给药7 d，使用自身对照法对大鼠进行单独的神经评分。结果体内实验中立即给予1 μmol/kg自由基多肽缀合物（JXY，Gly-Arg-Pro-Ala-Lys-Arg-Gly-Asp-Val）治疗急性缺血性脑卒中大鼠，或发病4、6和24 h后给药均可治愈。这种优势在以JXY 1 μmol/kg为初始剂量并持续给药时被进一步增强。相反，3 mg/kg t-PA没有这样的治疗效果。结论急性缺血性脑卒中发病超过3 h后的溶栓治疗中提高t-PA的疗效和限制其神经毒性具有重要的临床意义。急性缺血性脑卒中发病后4、6、24 h的大鼠模型具有广泛的用途。1 μmol/kg JXY在急性缺血性脑卒中大鼠模型中立即治疗或发病4、6、24 h后治疗均有效。

关键词: 缺血性中风, 大鼠模型, 行为, 不良反应

Abstract:

Objective To establish rat model of acute ischemic stroke of long-term symptom onset, as well as to evaluate therapeutic efficacy and side effects. Methods Individually neurological ratings were scored and self-contrast was performed, as well as continues administration were given for 6 consecutive days. Results In vivo 1 μmol/kg JXY(Gly-Arg-Pro-Ala-Lys-Arg-Gly-Asp-Val) treated rats of acute ischemic stroke immediately, or 4, 6 and 24 h after symptom onset were cured. This advantage was further enhanced by continuously administered JXY with 1 μmol/kg initial dose. In contrast, 3 mg/kg t-PA had no such therapeutic efficacy. Conclusion Improving efficacy and limiting the neurotoxicity of t-PA are of clinical importance to thrombolytic therapy in the treatment of acute ischemic stroke over 3 h symptom onset. The rat models of acute ischemic stroke of 4, 6 and 24 h symptom onset have broad utility. On the model 1 μmol/kg JXY is capable of treating acute ischemic stroke rats immediately, 4, 6 and 24 h after symptom onset.

Key words: ischemic stroke, experimental model, behavior, side effect

中图分类号:

R965.1

蒋雪云, 张颖洁, 赵明. 超黄金治疗期的缺血性中风大鼠模型、治疗模式及疗效表述[J]. 首都医科大学学报, 2014, 35(1): 108-112.

Jiang Xueyun, Zhang Yingjie, Zhao Ming. Long-term model, administration pattern and efficacy description of rats in acute ischemic stroke condition[J]. Journal of Capital Medical University, 2014, 35(1): 108-112.

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超黄金治疗期的缺血性中风大鼠模型、治疗模式及疗效表述

Long-term model, administration pattern and efficacy description of rats in acute ischemic stroke condition

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