脑血红蛋白表达增高可减轻α-突触核蛋白引起的MES23.5细胞凋亡

doi:10.3969/j.issn.1006-7795.2016.06.016

首都医科大学学报 ›› 2016, Vol. 37 ›› Issue (6): 799-805.doi: 10.3969/j.issn.1006-7795.2016.06.016

脑血红蛋白表达增高可减轻α-突触核蛋白引起的MES23.5细胞凋亡

杨巍巍^1,2, 李旭冉^1,2, 李昕^1,2, 李旭颖^1,2, 于顺^1,2,3

1. 首都医科大学宣武医院神经生物学研究室, 北京 100053;
2. 帕金森病研究北京重点实验室, 北京 100053;
3. 北京脑重大疾病研究院帕金森病研究所, 北京 100053

收稿日期:2016-02-14 出版日期:2016-12-21 发布日期:2016-12-16
通讯作者: 于顺 E-mail:yushun103@163.com
基金资助:
国家重点基础研究发展计划（2011CB504101），国家自然科学基金（81071014，81371200，81401042），国家科技支撑计划课题（2012BAI10B03），首都卫生发展科研专项课题（2011-4001-01），北京市自然科学基金（7122035）

Expression of neuronal hemoglobin alleviates α-synuclein-induced apoptosis in MES23.5 cells

Yang Weiwei^1,2, Li Xuran^1,2, Li Xin^1,2, Li Xuying^1,2, Yu Shun^1,2,3

1. Department of Neurobiology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China;
2. Key Laboratory of Neurodegenerative Disease, Ministry of Education, Beijing 100053, China;
3. Center for Parkinson's Disease, Beijing Institute for Brain Disorders, Beijing 100053, China

Received:2016-02-14 Online:2016-12-21 Published:2016-12-16
Supported by:
This study was supported by Major State Basic Research Development Program ("973" Program) of China (2011CB504101), National Natural Science Foundation of China (81071014, 81371200, 81401042), National Science and Technology Support Program (2012BAI10B03),The Capital Health Research and Development Special Fund (2011-4001-01), Natural Science Foundation of Beijing (7122035).

摘要/Abstract

摘要： 目的观察脑血红蛋白（neuronal hemoglobin，nHb）在α-突触核蛋白（α-synuclein，α-syn）引起的细胞凋亡中的作用。方法在MES23.5细胞中瞬时转染空载（myc）和nHb质粒，部分转染细胞外加α-syn，免疫荧光化学方法鉴定基因表达情况。Western blotting法检测α-syn与nHb表达水平。JC-1检测各组细胞线粒体膜电势水平，TUNEL染色和MTT法检测各组细胞死亡情况。结果过表达nHb可与α-syn在胞质及线粒体均形成复合物，并显著降低游离α-syn在胞质及线粒体的水平，同时，nHb过表达可显著逆转α-syn所致线粒体膜电势下降及细胞凋亡。结论 nHb表达增高可缓解α-syn致细胞损伤。

关键词: α-突触核蛋白, 血红蛋白, 线粒体, 凋亡

Abstract: Objective To explore the effect of neuronal hemoglobin (nHb) on α-synuclein(α-syn)-induced cell apoptosis. Methods myc vector or nHb was transiently transfected into MES23.5 cells with or without addition of exogenous α-syn. As identification of gene expression by immunofluorescence, Western blotting was used to detect the expression of α-syn and nHb. JC-1 staining was used to detect the mitochondrial membrane potential. The cell injuries were observed by TUNEL staining and MTT assay. Results nHb gene overexpression in MES23.5 cells lead to formation the complexes with α-syn in cytosolic and mitochondrial fraction, and reduced free nHb levels. Meanwhile, nHb could reverse α-syn-induced reduction of mitochondrial membrane potential and cell apoptosis. Conclusion Increase of nHb could alleviate α-syn-induced cell injuries.

Key words: α-synuclein (α-syn), hemoglobin, mitochondria, apoptosis

中图分类号:

R338

杨巍巍, 李旭冉, 李昕, 李旭颖, 于顺. 脑血红蛋白表达增高可减轻α-突触核蛋白引起的MES23.5细胞凋亡[J]. 首都医科大学学报, 2016, 37(6): 799-805.

Yang Weiwei, Li Xuran, Li Xin, Li Xuying, Yu Shun. Expression of neuronal hemoglobin alleviates α-synuclein-induced apoptosis in MES23.5 cells[J]. Journal of Capital Medical University, 2016, 37(6): 799-805.

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脑血红蛋白表达增高可减轻α-突触核蛋白引起的MES23.5细胞凋亡

Expression of neuronal hemoglobin alleviates α-synuclein-induced apoptosis in MES23.5 cells

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