复苏后常温条件下卵巢组织活性与时间关系的初步探究

doi:10.3969/j.issn.1006-7795.2019.04.010

首都医科大学学报 ›› 2019, Vol. 40 ›› Issue (4): 544-548.doi: 10.3969/j.issn.1006-7795.2019.04.010

复苏后常温条件下卵巢组织活性与时间关系的初步探究

金凤羽¹, 阮祥燕^1,2, Alfred O. Mueck^1,2, 杜娟¹, 李扬璐¹, 程姣姣¹, 王虎生¹

1. 首都医科大学附属北京妇产医院内分泌科, 北京 100026;
2. 德国图宾根大学妇产医院妇女健康部与妇女健康研究中心, 图宾根 D-72076, 德国

收稿日期:2019-05-20 出版日期:2019-07-21 发布日期:2019-07-19
通讯作者: 阮祥燕 E-mail:ruanxiangyan@163.com
基金资助:
北京市自然科学基金（Y181004），首都临床特色应用研究与成果推广项目（Z161100000516143），首都卫生发展科研专项项目（2016-2-2113），北京市医院管理局"登峰"计划专项（DFL20181401），北京市属医院科研培育计划项目（PZ2019031），国家外专局引智重点项目（20181100005），北京市卫生系统高层次卫生技术人才（2014-2-016），北京市医院管理局临床技术创新项目（XMLX201710）。

A study on the relationship between ovarian tissue viability and time under normal temperature after thawing

Jin Fengyu¹, Ruan Xiangyan^1,2, Alfred O. Mueck^1,2, Du Juan¹, Li Yanglu¹, Cheng Jiaojiao¹, Wang Husheng¹

1. Department of Gynecological Endocrinology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China;
2. Department for Women's Health, University Women's Hospital and Research Center for Women's Health, University of Tuebingen, Tuebingen D-72076, Germany

Received:2019-05-20 Online:2019-07-21 Published:2019-07-19
Supported by:
This study was supported by Nature Science Foundation of Beijing(Y181004), Foundation of Beijing Municipal Science & Technology Commission (Z161100000516143), Beijing Capital's Foundation for Science Development and Research (2016-2-2113), Beijing Municipal Administration of Hospitals' Ascent Plan (DFL20181401), Beijing Municipal Administration of Hospitals Incubating Program (PZ2019031),SAFEA:Project for Key Foreign Experts (20181100005), Beijing Municipality Health Technology High-level Talent (2014-2-016), Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding (XMLX201710).

摘要/Abstract

摘要： 目的对复苏后常温存放不同时间段的卵巢组织活性进行测定，评估常温条件下存放时间对复苏卵巢组织活性的影响，进而探索短程转运复苏后卵巢组织的可行性。方法 13位患者，每位患者取直径2 mm的标本共9个，其中一个用于新鲜卵巢组织卵泡计数，剩余8个标本进行程序化冷冻/复苏，根据复苏后常温存放时间的不同分为4组，即组1（0 min）、组2（20 min）、组3（40 min）和组4（60 min）。对比新鲜组织组与复苏后4组样本的卵泡计数，以及复苏后4组间雌激素、乳酸和葡萄糖代谢指标的差异。结果新鲜组织组和复苏后组1之间，复苏后4组之间卵泡计数差异无统计学意义（P>0.05）。复苏后4组之间雌激素浓度、乳酸以及葡萄糖代谢水平差异无统计学意义（P>0.05）。结论复苏后常温条件下1 h内卵巢组织活性无明显下降。

关键词: 卵巢组织冻存, 生殖力保护, 复苏, 卵泡计数

Abstract: Objective To estimate the thawed ovarian tissue viability and explore the feasibility of short distance transportation after thawing different periods at room temperature conditions. Methods Thirteen patients in total,9 samples (2 mm in diameter) were obtained from each patient. One fresh sample was performed follicle counts and the other 8 samples were cryopreservatethawed and randomly divided into 4 groups depends on the time after thawing,that means group 1(0 min), group 2(20 min), group 3(40 min), group 4(60 min). Viable follicle counts,steroid hormone lactic acid and glucose metabolism were performed. Results There were no statistical differences in follicles viability between the fresh group and 4 groups after thawing. And no statistical differences in follicles viability,steroid hormone, lactic acid and glucose metabolism between 4 groups after thawing. Conclusion The ovarian tissue viability did not be impaired after thawing at room temperature in one hour.

Key words: ovarian tissue preservation, fertility protection, thawing, follicle counts

中图分类号:

R711.77

金凤羽, 阮祥燕, Alfred O. Mueck, 杜娟, 李扬璐, 程姣姣, 王虎生. 复苏后常温条件下卵巢组织活性与时间关系的初步探究[J]. 首都医科大学学报, 2019, 40(4): 544-548.

Jin Fengyu, Ruan Xiangyan, Alfred O. Mueck, Du Juan, Li Yanglu, Cheng Jiaojiao, Wang Husheng. A study on the relationship between ovarian tissue viability and time under normal temperature after thawing[J]. Journal of Capital Medical University, 2019, 40(4): 544-548.

参考文献 13

[1]	Furlong W,Rae C,Greenberg M L,et al. Surveillance and survival among adolescents and young adults with cancer in Onrario,Canada[J]. Int J Cancer,2012,131(11):2660-2667.
[2]	Ruan X. Chinese Society of Gynecological Endocrinology Affiliated to the International Society of Gynecological Endocrinology Guideline for ovarian tissue cryopreservation and transplantation[J]. Gynecol Endocrinol, 2018,34(12):1005-1010.
[3]	Dittrich R,Kliesch S,Schüring A,et al. Fertility preservation for patients with malignant disease. Guideline of the DGGG,DGU and DGRM (S2k-Level,AWMF Registry No.015/082,November 2017) - Recommendations and Statements for Girls and Women[J]. Geburtshlife Frauenheilkd,2018,78(6):567-584.
[4]	Donnez J,Dolmans M M,Diaz C,et al. Ovarian cortex transplantation:time to move on from experimental studies to open clinical application[J]. Fertil Steril,2015,104(5):1097-1098.
[5]	Lambertini M,Del Mastro L,Pescio M C,et al. Cancer and fertility preservation:international recommendations from an expert meeting[J]. BMC Med, 2016,14:1
[6]	Kim S K,Youm H W,Lee J R,et al. Chapter 4 role of antioxidants and antifreeze proteins in cryopreservation/[J]itrification[J]. Methods Mol Biol,2017,1568:45-63.
[7]	Donnez J,Dolmans M M. Ovarian cortex transplantation:60 reported live births brings the success and worldwide expansion of the technique towards routine clinical practice[J]. J Assist Reprod Genet,2015,32(8):1167-1170.
[8]	Isachenko E,Isachenko V,Nawroth F,et al. Effect of long-term exposure at suprazero temperatures on activity and viability of human ovarian cortex[J]. Fertil Steril,2009,91(Suppl 4):1556-1559.
[9]	Klocke S,Bundgen N,Koster F,et al. Slow-freezing versus vitrification for human ovarian tissue cryopreservation[J]. Arch Gynecol Obstet,2015,291(2):419-426.
[10]	Gerritse R,Beerendonk C C,Westphal J R,et al. Glucose/lactate metabolism of cryopreserved intact bovine ovaries as a novel quantitative marker to assess tissue cryodamage[J]. Reprod Biomed Online,2011,23(6):755-756.
[11]	Chambers E L,Gosden R G,Yap C,et al. In situ identification of follicles in ovarian cortex as a tool for quantifying follicle density,viability and developmental potential in strategies to preserve female fertility[J]. Hum Reprod,2010,25(10):2559-2568.
[12]	Milenkovic M,Diaz-Garcia C,Wallin A,et al. Viability and function of the cryopreserved whole rat ovary:comparison between slow-freezing and vitrification[J]. Fertil Steril,2012,97(5):1176-1182.
[13]	程姣姣, 阮祥燕,李扬璐,等.北京及周边地区早发性卵巢功能不全的病因学研究[J].首都医科大学学报,2018,39(4):494-498.

复苏后常温条件下卵巢组织活性与时间关系的初步探究

A study on the relationship between ovarian tissue viability and time under normal temperature after thawing

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献 13

相关文章 11

编辑推荐

Metrics

本文评价

[1]	程姣姣, 阮祥燕, 杜娟, 谷牧青. 未成熟卵母细胞体外成熟在生育力保护中的应用进展[J]. 首都医科大学学报, 2022, 43(3): 329-335.
[2]	程姣姣, 阮祥燕, 杜娟, 金凤羽, 李扬璐, 谷牧青. 卵巢组织冻存移植安全性的研究进展[J]. 首都医科大学学报, 2021, 42(4): 505-510.
[3]	金凤羽, 阮祥燕, 杜娟, 程姣姣, 李扬璐, 王虎生, 谷牧青, 鞠蕊, 杨瑜, 王月姣, 许新. 中国首个卵巢组织冻存库冻存患者特征及冻存效果分析[J]. 首都医科大学学报, 2021, 42(4): 521-525.
[4]	阮祥燕, 杨瑜, 卢丹, 杜娟, 程姣姣, 谷牧青, 李扬璐, 金凤羽, 段微, 代荫梅, 鞠蕊, 许新, MatthiasKorell, AlfredO.Mueck. 中国首例乳腺癌患者冻存卵巢组织移植成功报道及文献复习[J]. 首都医科大学学报, 2021, 42(2): 177-182.
[5]	程姣姣, 阮祥燕, 杜娟, 谷牧青. 间充质干细胞对人冻融卵巢组织移植后低氧期保护作用的研究进展[J]. 首都医科大学学报, 2020, 41(4): 536-541.
[6]	程姣姣, 阮祥燕, 周琦, 李扬璐, 杜娟, 金凤羽, 谷牧青, Alfred O. Mueck. 大鼠卵巢组织体积对其动情周期及体质量的影响[J]. 首都医科大学学报, 2019, 40(4): 554-559.
[7]	李扬璐, 阮祥燕, Alfred O. Mueck. 人卵巢组织冻存与移植研究进展[J]. 首都医科大学学报, 2017, 38(4): 485-491.
[8]	王烁, 李春盛. 通气与否？心肺复苏的新策略[J]. 首都医科大学学报, 2015, 36(3): 491-496.
[9]	杭晨晨, 李春盛, 吴彩军, 杨军, 张奕, 郭志军, 殷勤. 腹部加压联合胸外按压对窒息猪复苏效果的研究[J]. 首都医科大学学报, 2013, 34(3): 441-445.
[10]	张婷;王晓民. 解读《时代》周刊2010年度十大医学突破[J]. 首都医科大学学报, 2011, 32(1): 159-163.
[11]	曾翔俊;张立克;芦玲巧;王红霞. 11, 12-环氧二十碳三烯酸对心肺复苏术的影响[J]. 首都医科大学学报, 2002, 23(4): 297-299.