大鼠卵巢组织体积对其动情周期及体质量的影响

doi:10.3969/j.issn.1006-7795.2019.04.012

首都医科大学学报 ›› 2019, Vol. 40 ›› Issue (4): 554-559.doi: 10.3969/j.issn.1006-7795.2019.04.012

大鼠卵巢组织体积对其动情周期及体质量的影响

程姣姣¹, 阮祥燕^1,2, 周琦³, 李扬璐¹, 杜娟¹, 金凤羽¹, 谷牧青⁴, Alfred O. Mueck^1,2

1. 首都医科大学附属北京妇产医院内分泌科, 北京 100026;
2. 德国图宾根大学妇产医院妇女健康部与妇女健康研究中心, 图宾根 D-72076, 德国;
3. 首都医科大学附属北京妇产医院妇科, 北京 100026;
4. 首都医科大学附属北京妇产医院生殖医学科, 北京 100026

收稿日期:2019-05-20 出版日期:2019-07-21 发布日期:2019-07-19
通讯作者: 阮祥燕 E-mail:jiachanwei@163.com
基金资助:
首都临床特色应用研究与成果推广项目（Z161100000516143），北京市医院管理局"登峰"计划专项经费资助（DFL20181401），北京市医院管理局临床技术创新项目（XMLX201710），首都卫生发展科研专项（2016-2-2113），国家外专局引智重点项目（20181100005），医疗健康技术创新与人才培养专项基金（2017041900004，2018042000001），北京市卫生系统高层次卫生技术人才项目（2014-2-016）。

Study on the effect of the ovarian tissue volume on estrous cycle and body weight in rats

Cheng Jiaojiao¹, Ruan Xiangyan^1,2, Zhou Qi³, Li Yanglu¹, Du Juan¹, Jin Fengyu¹, Gu Muqing⁴, Alfred O. Mueck^1,2

1. Department of Gynecological Endocrinology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China;
2. Department for Women's Health, University Women's Hospital and Research Center for Women's Health, University of Tuebingen, Tuebingen D-72076, Germany;
3. Department of Gynecology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China;
4. Department of Reproductive Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China

Received:2019-05-20 Online:2019-07-21 Published:2019-07-19
Supported by:
This study was supported by Foundation of Beijing Municipal Science and Technology Commission (Z161100000516143), Beijing Municipal Administration of Hospitals' Ascent Plan (DFL20181401), Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support (XMLX201710),Beijing Capital Foundation for Medical Science Development and Research (2016-2-2113), SAFEA:Project for Key Foreign Experts (20181100005), Medical Health Technology Innovation and Talent Training Special Fund (2017041900004, 2018042000001), Beijing Municipality Health Technology High-level Talent (2014-2-016).

摘要/Abstract

摘要： 目的观察切除大鼠不同卵巢体积对其动情周期及体质量的影响。方法 67只10周龄SD大鼠适应1周后，按照切除不同的卵巢体积共分为6组：A组，假手术组（n=12）；B组，切除单侧1/2（n=12）；C组，切除单侧全部（n=12）；D组，切除单侧全部+对侧1/2（n=12）；E组，切除单侧全部+对侧3/4（n=12）；F组，切除双侧全部（n=7）。卵巢部分切除术后4、8、12周连续9 d监测大鼠阴道细胞涂片，并于手术当天、术后4、6、8、10、12周监测大鼠体质量。分析切除大鼠不同卵巢体积对其动情周期及体质量的影响。结果 E组以及F组大鼠的动情周期与其余4组间差异有统计学意义（P<0.05），动情周期天数高于其余4组。F组大鼠体质量明显高于其余5组（P<0.05）。结论大鼠卵巢切除超过单侧全部+对侧3/4组时对动情周期有影响，双侧全切对体质量有影响。

关键词: 卵巢体积, 动情周期, 卵巢组织冻存, 早发性卵巢功能不全

Abstract: Objective To investigate the effect of different ovarian tissue volume on estrous cycle and body weight in rats. Methods Totally 67 10-week-old Sprague-Dawley rats were divided into 6 groups according to removed different ovarian tissue volume after one week's acclimatization. Group A,sham operation group (n=12), Group B,the half of left ovary removed (n=12),Group C,the left whole ovary (n=12),Group D,the left whole ovary and the half of right ovary removed (n=12),Group E,the left whole ovary and the three quarters of right ovary removed (n=12),Group F,both left and right ovaries removed (n=7). The vaginal smear of the rats was monitored for 9 consecutive days at 4 weeks,8 weeks,and 12 weeks after partial ovarian resection,and the body weight of the rats was monitored on the day of surgery,4 weeks,6 weeks,8 weeks,10 weeks,and 12 weeks after surgery. The effects of the ovarian volumes on the estrous cycle and body weight of the rats were analyzed. Results There was a statistically significant difference in estrous cycle between the E group and the F group and the other 4 groups (P<0.05),and the estrous cycle days of Group E,Group F were higher than that of the other 4 groups. Rats of weight in Group F is higher than that in the other 5 groups (P<0.05). Conclusion Removed one and three quarters of ovary imposed an effect on the rat's estrous cycle, and bilateral oophorectomy imposed an effect on rat's body weight.

Key words: ovarian volume, estrous cycle, ovarian tissue cryopreservation, premature ovarian insufficiency

中图分类号:

R711.77

程姣姣, 阮祥燕, 周琦, 李扬璐, 杜娟, 金凤羽, 谷牧青, Alfred O. Mueck. 大鼠卵巢组织体积对其动情周期及体质量的影响[J]. 首都医科大学学报, 2019, 40(4): 554-559.

Cheng Jiaojiao, Ruan Xiangyan, Zhou Qi, Li Yanglu, Du Juan, Jin Fengyu, Gu Muqing, Alfred O. Mueck. Study on the effect of the ovarian tissue volume on estrous cycle and body weight in rats[J]. Journal of Capital Medical University, 2019, 40(4): 554-559.

参考文献 32

[1]	Webber L,Davies M,Anderson R,et al. ESHRE Guideline:management of women with premature ovarian insufficiency[J]. Human Reprod(Oxford,England),2016,31(5):926-937.
[2]	陈子江,田秦杰,乔杰,等. 早发性卵巢功能不全的临床诊疗中国专家共识[J]. 中华妇产科杂志,2017,52(9):577-581.
[3]	程姣姣,阮祥燕,李扬璐,等. 北京及周边地区早发性卵巢功能不全的病因学研究[J]. 首都医科大学学报,2018,4(39):494-498.
[4]	程姣姣,阮祥燕,冯欣,等. 生育力保护保存与早发性卵巢功能不全的防治[J]. 中国临床医生杂志,2018,16(3):1-5.
[5]	Tsiligiannis S,Panay N,Stevenson J C. Premature ovarian insufficiency and long-term health consequences[J]. Curr Vasc Pharmacol,2019.
[6]	Spath M A,Braat D D M. Iatrogenic and non-iatrogenic causes of female fertility loss that may indicate fertility preservation[J]. Acta Obstet Gynecol Scand,2019,98(5):559-562.
[7]	Simpson J L,Ory S J. Can pregnancies be achieved in premature ovarian insufficiency?[J]. Reprod Sci,2019,26(2):157-158.
[8]	阮祥燕. 卵巢组织冻存与移植中国专家共识[J]. 中国临床医生杂志,2018,46(4):496-500.
[9]	Ruan X. Chinese society of gynecological endocrinology affiliated to the international society of gynecological endocrinology guideline for ovarian tissue cryopreservation and transplantation[J]. Gynecol Endocrinol,2018,34(12):1005-1010.
[10]	Forman E J. Ovarian tissue cryopreservation:still experimental?[J]. Fertil Steril,2018,109(3):443-444.
[11]	Sanger N,Jarisch A,Ochsendorf F,et al. Fertility preservation in prepubertal und pubertal children and adolescents[J]. Klin Padiatr,2018,78:567-584.
[12]	Wallace W H B,Smith A G,Kelsey T W,et al. Fertility preservation for girls and young women with cancer:population-based validation of criteria for ovarian tissue cryopreservation[J]. Lancet Oncol,2014,15(10):1129-1136.
[13]	Wallace W H B,Kelsey T W,Anderson R A. Fertility preservation in pre-pubertal girls with cancer:the role of ovarian tissue cryopreservation[J]. Fertil Steril,2016,105(1):6-12.
[14]	Jensen A K,Rechnitzer C,Macklon K T,et al. Cryopreservation of ovarian tissue for fertility preservation in a large cohort of young girls:focus on pubertal development[J]. Human Reprod,2017,32(1):154-164.
[15]	Yasmin E,Balachandren N,Davies M C,et al. Fertility preservation for medical reasons in girls and women:British fertility society policy and practice guideline[J]. Human Fertil,2018,21(1):3-26.
[16]	Corkum K S,Rhee D S,Wafford Q E,et al. Fertility and hormone preservation and restoration for female children and adolescents receiving gonadotoxic cancer treatments:A systematic review[J]. J Pediatr Surg,2019, Epub ahead of print.
[17]	Donnez J,Dolmans M. Fertility preservation in women[J]. N Engl J Med,2017,377(17):1657-1665.
[18]	Beckmann M W,Lotz L,Toth B,et al. Concept paper on the technique of cryopreservation,removal and transplantation of ovarian tissue for fertility preservation[J]. Geburtshilife Frauenheilkd,2019,79(1):53-62.
[19]	Rosendahl M,Simonsen M K,Kjer J J. The influence of unilateral oophorectomy on the age of menopause[J]. Climacteric,2017,20(6):540-544.
[20]	Cora M C,Kooistra L,Travlos G. Vaginal cytology of the laboratory rat and mouse:review and criteria for the staging of the estrous cycle using stained vaginal smears[J]. Toxicol Pathol,2015,43(6):776-793.
[21]	Dolmans M,Manavella D D. Recent advances in fertility preservation[J]. J Obstetr Gynaecol Rese,2018,45(2):266-279.
[22]	Meirow D,Ra'Anani H,Shapira M,et al. Transplantations of frozen-thawed ovarian tissue demonstrate high reproductive performance and the need to revise restrictive criteria[J]. Fertil Steril,2016,106(2):467-474.
[23]	Amato P, Brzyski R, Benward J, et al. Fertility preservation and reproduction in patients facing gonadotoxic therapies:a committee opinion[J]. Fertil Steril,2013,100(5):1224-1231.
[24]	Imbert R,Moffa F,Tsepelidis S,et al. Safety and usefulness of cryopreservation of ovarian tissue to preserve fertility:a 12-year retrospective analysis[J]. Hum Reprod,2014,29(9):1931-1940.
[25]	Kristensen S G,Andersen C Y. Cryopreservation of ovarian tissue:opportunities beyond fertility preservation and a positive view into the future[J]. Front Endocrinol,2018,9:347.
[26]	Gellert S E,Pors S E,Kristensen S G,et al. Transplantation of frozen-thawed ovarian tissue:an update on worldwide activity published in peer-reviewed papers and on the Danish cohort[J]. J Assist Reprod Genet,2018,35(4):561-570.
[27]	Van der Ven H,Liebenthron J,Beckmann M,et al. Ninety-five orthotopic transplantations in 74 women of ovarian tissue after cytotoxic treatment in a fertility preservation network:tissue activity,pregnancy and delivery rates[J]. Hum Reprod,2016,31(9):2031-2041.
[28]	阮祥燕,程姣姣,杜娟,等. 生育力保护方法研究进展[J]. 山东大学学报:医学版,2019,57(2):29-34.
[29]	Meirow D,Ra'anani H,Shapira M,et al. Transplantations of frozen-thawed ovarian tissue demonstrate high reproductive performance and the need to revise restrictive criteria[J]. Fertil Steril,2016,106(2):467-474.
[30]	Poirot C,Brugieres L,Yakouben K,et al. Ovarian tissue cryopreservation for fertility preservation in 418 girls and adolescents up to 15 years of age facing highly gonadotoxic treatment.Twenty years of experience at a single center[J]. Acta Obstetr Gynecol Scand,2019,98(5):630-637.
[31]	Donnez J,Dolmans M,Diaz C,et al. Ovarian cortex transplantation:time to move on from experimental studies to open clinical application[J]. Fertil Steril,2015,104(5):1097-1098.
[32]	Lizcano F,Guzman G. Estrogen deficiency and the origin of obesity during menopause[J]. Bio Med Res Int,2014,2014:757461.

大鼠卵巢组织体积对其动情周期及体质量的影响

Study on the effect of the ovarian tissue volume on estrous cycle and body weight in rats

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