外泌体miRNA在急性心肌梗死中的研究进展

doi:10.3969/j.issn.1006-7795.2022.02.006

摘要/Abstract

摘要： 外泌体是被细胞以胞吐形式释放到细胞间隙中的囊泡状结构,可以作为载体携带miRNA、蛋白质等物质远距离传输生物学信息。miRNA是内源性进化上高度保守的非编码小RNA,与靶mRNA特异性结合,导致翻译阻遏,调节转录后基因的表达,从而参与细胞的生长分化等病理生理过程。近年来研究显示外泌体可以借助miRNA调节多种信号通路,在急性心肌梗死的发生、发展中发挥重要作用,在诊断和治疗方面具有较大应用前景。本文将针对外泌体miRNA在急性心肌梗死中的研究进展进行简要概述。

关键词: 外泌体, miRNA, 急性心肌梗死

Abstract: Exosomes is extracellular vesicles that are released into the extracellular space by the way of exoplasmosis. Exosomes can be used as a carrier to carry miRNA, protein,etc and transmit biological information in long distance. miRNA are endogenous and evolutionarily highly conserved non-coding RNA that combine with target mRNA to form translational repressions to achieve regulation of post transcriptional gene expression, thereby participating in pathophysiological processes such as cell growth and differentiation. Recent studies have shown that exosomes plays a role in the process of acute myocardial infarction by carrying microRNA, and have large prospect in the diagnosis and treatment of acute myocardial infarction. Therefore, this article briefly summarizes the recent research progress of exosomes miRNA in acute myocardial infarction.

Key words: exosomes, miRNA, acute myocardial infarction

中图分类号:

R542.2

鲍博艺, 李卫萍. 外泌体miRNA在急性心肌梗死中的研究进展[J]. 首都医科大学学报, 2022, 43(2): 199-204.

Bao Boyi, Li Weiping. Research progress of exosomes miRNA in acute myocardial infarction[J]. Journal of Capital Medical University, 2022, 43(2): 199-204.

参考文献

[1] 国家心血管病中心. 中国心血管健康与疾病报告2020[J]. 心肺血管病杂志, 2021, 40(9): 885-889.
[2] Kalluri R, LeBleu V S. The biology, function, and biomedical applications of exosomes[J]. Science, 2020, 367(6478): eaau6977.
[3] Wang C X, Zhang C C, Liu L X, et al. Macrophage-derived mir-155-containing exosomes suppress fibroblast proliferation and promote fibroblast inflammation during cardiac injury[J]. Mol Ther, 2017, 25(1): 192-204.
[4] Liu S J, Chen J, Shi J, et al. M1-like macrophage-derived exosomes suppress angiogenesis and exacerbate cardiac dysfunction in a myocardial infarction microenvironment[J]. Basic Res Cardiol, 2020, 115(2): 22.
[5] Liu H B, Zhang Y M, Yuan J, et al. Dendritic cell-derived exosomal miR-494-3p promotes angiogenesis following myocardial infarction[J]. Int J Mol Med, 2021, 47(1): 315-325.
[6] 孙硕, 康品方, 张恒. 外泌体miRNA在心血管疾病中的研究进展[J]. 心血管病学进展, 2021, 42(3): 224-227.
[7] Aheget H, Mazini L, Martin F, et al. Exosomes: their role in pathogenesis, diagnosis and treatment of diseases[J]. Cancers, 2020, 13(1): 84.
[8] Mori M A, Ludwig R G, Garcia-Martin R, et al. Extracellular miRNAs: from biomarkers to mediators of physiology and disease[J]. Cell Metab, 2019, 30(4): 656-673.
[9] Singla D K. Stem cells and exosomes in cardiac repair[J]. Curr Opin Pharmacol, 2016, 27: 19-23.
[10] Mir R, Elfaki I, Khullar N, et al. Role of selected miRNAs as diagnostic and prognostic biomarkers in cardiovascular diseases, including coronary artery disease, myocardial infarction and atherosclerosis[J]. J Cardiovasc Dev Dis, 2021, 8(2): 22.
[11] Schulte C, Karakas M, Zeller T. MicroRNAs in cardiovascular disease-clinical application[J]. Clin Chem Lab Med, 2017, 55(5): 687-704.
[12] Shao L B, Zhang Y, Lan B B, et al. MiRNA-sequence indicates that mesenchymal stem cells and exosomes have similar mechanism to enhance cardiac repair[J]. Biomed Res Int, 2017, 2017: 4150705.
[13] Peng Y, Zhao J L, Peng Z Y, et al. Exosomal miR-25-3p from mesenchymal stem cells alleviates myocardial infarction by targeting pro-apoptotic proteins and EZH2[J]. Cell Death Dis, 2020, 11(5): 317.
[14] Cheng H, Chang S F, Xu R D, et al. Hypoxia-challenged MSC-derived exosomes deliver miR-210 to attenuate post-infarction cardiac apoptosis[J]. Stem Cell Res Ther, 2020, 11(1): 224.
[15] Luther K M, Haar L, McGuinness M, et al. Exosomal miR-21a-5p mediates cardioprotection by mesenchymal stem cells[J]. J Mol Cell Cardiol, 2018, 11: 125-137.
[16] Zhu L P, Tian T, Wang J Y, et al. Hypoxia-elicited mesenchymal stem cell-derived exosomes facilitates cardiac repair through miR-125b-mediated prevention of cell death in myocardial infarction[J]. Theranostics, 2018, 8(22): 6163-6177.
[17] Hao C S, Lu Z R, Zhao Y Y, et al. Overexpression of GATA4 enhances the antiapoptotic effect of exosomes secreted from cardiac colony-forming unit fibroblasts via miRNA221-mediated targeting of the PTEN/PI3K/AKT signaling pathway[J]. Stem Cell Res Ther, 2020, 11(1): 251.
[18] Ning W L, Li S H, Yang W G, et al. Blocking exosomal miRNA-153-3p derived from bone marrow mesenchymal stem cells ameliorates hypoxia-induced myocardial and microvascular damage by targeting the ANGPT1-mediated VEGF/PI3k/Akt/eNOS pathway[J]. Cell Signal, 2021, 77: 109812.
[19] Long R, Gao L, Li Y P, et al. M2 macrophage-derived exosomes carry miR-1271-5p to alleviate cardiac injury in acute myocardial infarction through down-regulating SOX6[J]. Mol Immunol, 2021, 136: 26-35.
[20] Wang B, Cao C, Han D J, et al. Dysregulation of miR-342-3p in plasma exosomes derived from convalescent AMI patients and its consequences on cardiac repair[J]. Biomed Pharmacother, 2021, 142: 112056.
[21] Geng T, Song Z Y, Xing J X, et al. Exosome derived from coronary serum of patients with myocardial infarction promotes angiogenesis through the miRNA-143/IGF-IR pathway[J]. Int J Nanomedicine, 2020, 15: 2647-2658.
[22] Gao L, Mei S Y, Zhang S N, et al. Cardio-renal exosomes in myocardial infarction serum regulate proangiogenic paracrine signaling in adipose mesenchymal stem cells[J]. Theranostics, 2020, 10(3): 1060-1073.
[23] Liao Z F, Chen Y L, Duan C C, et al. Cardiac telocytes inhibit cardiac microvascular endothelial cell apoptosis through exosomal miRNA-21-5p-targeted cdip1 silencing to improve angiogenesis following myocardial infarction[J]. Theranostics, 2021, 11(1): 268-291.
[24] Zhu W W, Sun L, Zhao P C, et al. Macrophage migration inhibitory factor facilitates the therapeutic efficacy of mesenchymal stem cells derived exosomes in acute myocardial infarction through upregulating miR-133a-3p[J]. J Nanobiotechnology, 2021, 19(1): 61.
[25] Kang J Y, Park H, Kim H, et al. Human peripheral blood-derived exosomes for microRNA delivery[J]. Int J Mol Med, 2019, 43(6): 2319-2328.
[26] Pan J J, Alimujiang M, Chen Q Y, et al. Exosomes derived from miR-146a-modified adipose-derived stem cells attenuate acute myocardial infarction-induced myocardial damage via downregulation of early growth response factor 1[J]. J Cell Biochem, 2019, 120(3): 4433-4443.
[27] Ke X, Yang R F, Wu F, et al. Exosomal miR-218-5p/miR-363-3p from endothelial progenitor cells ameliorate myocardial infarction by targeting the p53/JMY signaling pathway[J]. Oxid Med Cell Longev, 2021, 2021: 5529430.
[28] Vaskova E, Ikeda G, Tada Y, et al. Sacubitril/valsartan improves cardiac function and decreases myocardial fibrosis via downregulation of exosomal miR-181a in a rodent chronic myocardial infarction model[J]. J Am Heart Assoc, 2020, 9(13): e015640.
[29] Wei Z L, Qiao S H, Zhao J X, et al. MiRNA-181a over-expression in mesenchymal stem cell-derived exosomes influenced inflammatory response after myocardial ischemia-reperfusion injury[J]. Life Sci, 2019, 232: 116632.
[30] Zhao J X, Li X L, Hu J X, et al. Mesenchymal stromal cell-derived exosomes attenuate myocardial ischaemia-reperfusion injury through miR-182-regulated macrophage polarization[J]. Cardiovasc Res, 2019, 115(7): 1205-1216.
[31] Liu H Y, Yu L F, Zhou T G, et al. Lipopolysaccharide-stimulated bone marrow mesenchymal stem cells-derived exosomes inhibit H₂O₂-induced cardiomyocyte inflammation and oxidative stress via regulating miR-181a-5p/ATF2 axis[J]. Eur Rev Med Pharmacol Sci, 2020, 24(19): 10069-10077.
[32] Chen S Y, Fang H C, Liu R Z, et al. MiR-6718-5p and miR-4329 can be used as potential biomarkers for acute myocardial infarction[J]. J Card Surg, 2021, 36(10): 3721-3728.
[33] Ling H, Guo Z Y, Shi Y F, et al. Serum exosomal microRNA-21, microRNA-126, and PTEN are novel biomarkers for diagnosis of acute coronary syndrome[J]. Front Physiol, 2020, 11: 654.
[34] Su J, Li J Y, Yu Q L, et al. Exosomal miRNAs as potential biomarkers for acute myocardial infarction[J]. IUBMB Life, 2020, 72(3): 384-400.
[35] Guo M, Li R, Yang L F, et al. Evaluation of exosomal miRNAs as potential diagnostic biomarkers for acute myocardial infarction using next-generation sequencing[J]. Ann Transl Med, 2021, 9(3): 219.
[36] Zhao X X, Jia Y P, Chen H Z, et al. Plasma-derived exosomal miR-183 associates with protein kinase activity and may serve as a novel predictive biomarker of myocardial ischemic injury[J]. Exp Ther Med, 2019, 18(1): 179-187.
[37] Cortez-Dias N, Costa M C, Carrilho-Ferreira P, et al. Circulating miR-122-5p/miR-133b ratio is a specific early prognostic biomarker in acute myocardial infarction[J]. Circ J, 2016, 80(10): 2183-2191.
[38] Chachques J C, Gardin C, Lila N, et al. Elastomeric cardiowrap scaffolds functionalized with mesenchymal stem cells-derived exosomes induce a positive modulation in the inflammatory and wound healing response of mesenchymal stem cell and macrophage[J]. Biomedicines, 2021, 9(7): 824.
[39] Huang C Y,Neupane Y R, Lim X C, et al.Extracellular vesicles in cardiovascular disease[J]. Adv Clin Chem, 2021, 103: 47-95.