荧光原位杂交技术在膀胱尿路上皮癌术后复发的早期检测中的临床应用价值

doi:10.3969/j.issn.1006-7795.2014.03.007

首都医科大学学报 ›› 2014, Vol. 35 ›› Issue (3): 298-301.doi: 10.3969/j.issn.1006-7795.2014.03.007

• 泌尿外科基础临床研究 • 上一篇下一篇

荧光原位杂交技术在膀胱尿路上皮癌术后复发的早期检测中的临床应用价值

闫伟¹, 康雯婷², 陈山¹, 刘跃新¹

1. 首都医科大学附属北京同仁医院泌尿外科, 北京 100730;
2. 首都医科大学附属北京同仁医院中心实验室, 北京 100730

收稿日期:2014-03-19 出版日期:2014-06-21 发布日期:2014-06-14
通讯作者: 刘跃新，E-mail：doctorlyx@126.com E-mail:doctorlyx@126.com
基金资助:
首都医学发展科研基金（2009-3100）。

Clinical value of fluorescence in situ hybridization in early detection of postoperative recurrence of bladder urothelial cancer

Yan Wei¹, Kang Wenting², Chen Shan¹, Liu Yuexin¹

1. Department of Urology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China;
2. Central Laboratory of Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China

Received:2014-03-19 Online:2014-06-21 Published:2014-06-14
Supported by:
This study was supported by the Scienctific Research Foundation of Capital Medical Development(2009-3100).

摘要/Abstract

摘要：

目的应用荧光原位杂交技术（fluorescence in situ hybridization，FISH）对膀胱尿路上皮肿瘤患者术后复发进行早期评估预测。方法 2009年1月至 2011年11月，选取首都医科大学附属北京同仁医院40例临床诊断的高危膀胱尿路上皮癌患者，术后第3，6，9，12，18个月行尿细胞学检查、膀胱镜检查，并行尿液FISH检查，记录膀胱肿瘤复发的时间。观察FISH对于膀胱尿路上皮肿瘤患者术后复发的早期预测价值。结果 60%（12/20）的FISH阳性者膀胱肿瘤复发，FISH阴性者无1例出现膀胱肿瘤复发。FISH阳性者膀胱尿路上皮癌复发率高于FISH阴性者（60% vs 0%，P<0.05）。6例（50%）肿瘤复发患者FISH阳性先于膀胱镜检查及尿细胞学检查。结论 FISH对膀胱肿瘤检测的阳性率高于细胞学检测，FISH对膀胱肿瘤的诊断早于膀胱镜和细胞学。FISH技术可作为膀胱尿路上皮肿瘤术后复发的监测指标之一。

关键词: 膀胱癌, 荧光原位杂交技术, 复发, 预测

Abstract:

Objective To early diagnose postoperative recurrence of bladder urothelial cancer with fluorescence in situ hybridization(FISH).Methods Forty patients with bladder urothelial cancer were chosen between January, 2009 and November, 2011. Urine cytologic examination, cystoscopy, and FISH were performed in these patients at 3, 6, 9, 12, and 18 months post-operation, and tumor recurrence was recorded. Results Among the 40 patients tested, 12 of 20 FISH-positive patients developed tumor recurrence within the follow-up period, while none of the FISH-negative patients developed recurrence during the same period. The recurrence rate in FISH-positive patients(60%) was significantly higher than that of FISH-negative patients(P<0.05). The sensitivity to detect tumor recurrence by FISH analysis was higher than that by cystoscopy and urine cytologic examination. Conclusion FISH analysis is effective in early detection of postoperative recurrence of bladder urothelial cancer.

Key words: bladder cancer, fluorescence in situ hybridization, recurrence, prediction

中图分类号:

闫伟, 康雯婷, 陈山, 刘跃新. 荧光原位杂交技术在膀胱尿路上皮癌术后复发的早期检测中的临床应用价值[J]. 首都医科大学学报, 2014, 35(3): 298-301.

Yan Wei, Kang Wenting, Chen Shan, Liu Yuexin. Clinical value of fluorescence in situ hybridization in early detection of postoperative recurrence of bladder urothelial cancer[J]. Journal of Capital Medical University, 2014, 35(3): 298-301.

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荧光原位杂交技术在膀胱尿路上皮癌术后复发的早期检测中的临床应用价值

Clinical value of fluorescence in situ hybridization in early detection of postoperative recurrence of bladder urothelial cancer

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