Magnolol pretreatment prevents sepsis-induced intestinal dysmotility by maintaining functional interstitial cells of Cajal

doi:10.3969/j.issn.1006-7795.2013.02.001

Abstract

Abstract:

Objective To investigate the mechanism by which magnolol treatment prevents sepsis-induced dysmotility in lipopolysaccharide(LPS)-treated mice. Methods Sepsis was induced by intravenous(tail vein) injection of LPS. Animals were divided into four groups: the magnolol-treated septic group, the mosapride-treated septic group,the placebo-treated septic group, and the control group. Intestinal transit and circular smooth muscle contraction were measured 12 h after LPS injection, and immunocytochemisty was performed to study the morphology of ICCs. NO content, superoxide dismutase(SOD) activity, and malondialdehyde(MDA) concentration were detected using commercial kits. Results Intestinal transit and muscular contractility were significantly lower in the LPS-treated group than in the control group. Immunocytochemical experiments showed that the total number of ICCs, and the total and average lengths of the ICC processes were significantly decreased in the LPS-treated group compared with those in the control group. In LPS-treated animals, magnolol pretreatment significantly accelerated intestinal transit, increased circular muscle contraction, and prevented ICC morphological changes. Magnolol pretreatment prevented the sepsis-induced increase in NO concentration, and MDA concentration, and decrease in SOD activity in LPS-treated animals. Conclusion Magnolol treatment prevented sepsis-induced intestinal dysmotility by regulating oxidative stress to maintain functional ICCs.

Key words: Cajal cells, magnolol, nitric oxide, oxidative stress

CLC Number:

R969.4

MIAO Bin, ZHANG Shuwen, WANG Hong, YANG Tiecheng, ZHOU Deshan, WANG Baoen. Magnolol pretreatment prevents sepsis-induced intestinal dysmotility by maintaining functional interstitial cells of Cajal[J]. Journal of Capital Medical University, 2013, 34(2): 163-170.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2013.02.001

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2013/V34/I2/163

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