Isolation and identification of pseudomonas aeruginosa phages with broad host range in hospital

doi:10.3969/j.issn.1006-7795.2013.05.019

Abstract

Abstract:

Objective To isolate, screen pseudomonas aeruginosa phages with broad host range from hospital sewage wastewater and test their host spectrum to daily clinical isolates of pseudomonas aeruginosa to explore the application value in clinical antibiotic therapy. Methods pseudomonas aeruginosa phages were isolated and identified with plague method and their host spectrum were screened using the same method, the homology of the host bacteria were analysed by Rep-PCR in order to confirm the host spectrum at gene level; The lysis coverage of the combination of the broad host range phages were tested using plague method. Results Fourteen pseudomonas aeruginosa phages were isolated from sewage wastewater of sixteen hospitals, among the fourteen phages there were four phages with broad host range. The lysis coverage rate of the combination of the four broad host range phages to thirty pseudomonas aeruginosa strains isolated from daily test was 96.7%, to the twelve Imipenem-resistant ones in the thirty pseudomonas aeruginosa strains was 91.7%, to all the drug susceptive ones was 100%, and to other drug resistant ones was 100%. Conclusion Four pseudomonas aeruginosa phages with broad host range were isolated from fourteen pseudomonas aeruginosa phages. The combination of the four broad host range phages can lyse twenty-nine in thirty strains isolated from daily clinical test, the lysis coverage rate of the combination of the four broad host range phages to Imipenem-resistant pseudomonas aeruginosa and other drug resistant pseudomonas aeruginosa was above 90%, the combination had a high lysis coverage rate and this can lay the foundation and provide feasibility for clinical antibiotic therapy.

Key words: broad host range, pseudomonas aeruginosa phages, pseudomonas aeruginosa, drug resistant strains

CLC Number:

R446.5

DAI Fangfang, ZHAO Xiuying, YU Yanhua, ZHANG Lili, CHEN Ming, YUAN Xingxing, LI Ning. Isolation and identification of pseudomonas aeruginosa phages with broad host range in hospital[J]. Journal of Capital Medical University, 2013, 34(5): 727-732.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2013.05.019

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2013/V34/I5/727

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