Study on the virulence features of carbapenem-resistant Klebsiella pneumoniae from invasive abdomen infections

doi:10.3969/j.issn.1006-7795.2021.05.007

Abstract

Abstract: Objective To investigate the virulence features of carbapenem-resistant Klebsiella pneumoniae (CRKP). Methods A total of 38 CRKP clinical strains isolated from invasive abdomen infections were collected. The polymerase chain reaction (PCR) were performed to detect carbapenemase genes (blaKPC-2、blaNDM-1、blaIMP-4、blaVIM-1 and bla_OXA-48), virulence gene (rmpA2) and siderophore gen e(iucA). The CRKP strains were characterized wtih their multilocus sequence typing (MLST) . The capacity of biofilm formation and siderophore production of the isolates were quantitatively tested by crystal violet method and chrome azurol S (CAS) assay, respectively. Results Among 38 CRKPs, 25 strains (25/38, 78.1%) belonged to ST11, which was the predominate clone. Twenty-two strains(22/25, 88.0%)in ST11 group carried bla_KPC-2 only, with the percentage significant higher than that in non-ST11 group. Fifteen ST11 CRKPs (15/25, 60.0%) simultaneously possessed rmpA2 and iucA, and there had no statistical difference between ST11 group and non-ST11 group. Biofilm formation capacity were weaker in non-ST11 group and KPC+rmpA2+iucA group, without statistical differences. The CAS assay showed that the capacity of siderophore production in ST11 group and KPC+rmpA2+iucA group were significantly stronger than that in non-ST11 group and KPC group, respectively. Conclusion The coexistence of bla_KPC-2, rmpA2, and iucA in ST11 CRKPs indicated that strict control measures should be taken to prevent further dissemination.

Key words: Klebsiella pneumoniae, carbapenem-resistant, virulence, biofilm, siderophore

CLC Number:

R446.5

Ma Liyan, Sun Wei, Xia Shuai, Su Jianrong. Study on the virulence features of carbapenem-resistant Klebsiella pneumoniae from invasive abdomen infections[J]. Journal of Capital Medical University, 2021, 42(5): 733-738.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2021.05.007

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2021/V42/I5/733

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