Effect of Magnolia officinalis decoction on Candida albicans and its biofilm

doi:10.3969/j.issn.1006-7795.2021.05.015

Abstract

Abstract: Objective To observe the effects of Magnolia officinalis decoction on the formation of germ tube, the early adhesion gene and mature biofilm of Candida albicans(C.albicans). Methods Bud tube formation test was used to detect the effect of Magnolia officinalis decoction on the formation of bud tube C. albicans. The expression of agglutinin-like sequencefamily (ALS1), ALS2 and ALS3 genes was detected in real-time using fluorescence quantitative real-time PCR (qRT PCR). The effects of Magnolia officinalis decoction on the biofilm of C. albicansat maturity were observed using fluorescence microscope and scanning electron microscope(SEM). The effect of Magnolia officinalis decoction on bacterial activity in biofilm was observed by confocal microscope. Results The results obtained from the formation of germ tube test showed that Magnolia officinalis decoction could inhibit the formation of germ tube C.albicans in a concentration dependent manner (P<0.05). Magnolia officinalis decoction could reduce the expression of ALS1, ALS2 and ALS3 genes in a concentration dependent manner (P<0.05).Under fluorescence microscope, the biofilm in the blank control group was mainly mycelium. The biofilm structure was destroyed after the Magnolia officinalis decoction for 24, 48 and 72. SEM results also showed that Magnolia officinalis decoction could result in the destruction of the biofilm structure. Confocal microscopy showed that C. albicans in the blank control group was mainly living bacteria, and the number of dead bacteria increased significantly after 48 hours of Magnolia officinalis decoction at 15.625 mg/L. Conclusion The Magnolia officinalis decoction can inhibit the early adhension of C. albicans by down regulating ALS1, ALS2 and ALS3 genes.At the same time,it can promote the entry of drugs into biofilm by destroying the structure of mature biofilm in inhibit the formation of germ tube and play a bactericidal role.

Key words: Mangnolia officinalis, Candida albicans, germ tube, biofilm, traditional Chinese medicine decoction

CLC Number:

Xiang Xiaobo, Yan Lyubing, Zhou Yanmeng. Effect of Magnolia officinalis decoction on Candida albicans and its biofilm[J]. Journal of Capital Medical University, 2021, 42(5): 790-798.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2021.05.015

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2021/V42/I5/790

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