Omi/HtrA2 overexpression is associated with myocardial aging

doi:10.3969/j.issn.1006-7795.2021.05.014

Abstract

Abstract: Objective To observe the effect of increased serine protease Omi/HtrA2 on myocardial aging. Methods Overexpressed cardiac-specific Omi/HtrA2 transgenic mice was constructed, the expression of senescence markers p53, p21 and p16 were detected by Western blotting, and the correlation between Omi/HtrA2 and the senescence markers was analyzed. The aging indicators were detected in overexpressed Omi/HtrA2 H9c2 cells (Omi group) by Western blotting. The senescent cells were detected by β-galactosidase staining. Compared with overexpressed Omi/HtrA2 cells, the expression of senescence markers and the β-galactosidase positive senescent cells were detected after inhibiting the function of Omi/HtrA2 by Omi/HtrA2 specific inhibitor UCF101. Results (1) Compared with the control group, the expression of Omi/HtrA2 was significantly increased in the heart of myocardium-specific overexpressed Omi/HtrA2 transgenic mice (P<0.01). The aging markers p53, p21 and p16 were significantly increased in Omi/HtrA2 overexpressed group(P<0.01). (2) The expression of Omi/HtrA2 was positively correlated with the aging markers p53, p21, and p16 (P<0.01). (3) Compared with H9c2 cells (control group), expression of the senescence markers p53, p21 and p16 and the β-galactosidase positive senescent cells increased in Omi group (P<0.05). Compared with Omi group, expression of the senescence markers and the β-galactosidase positive senescent cells decreased in Omi/HtrA2 H9c2 cells treated by Omi/HtrA2 specific inhibitor UCF101 (Omi+UCF101 group) (P<0.05). Conclusion The expression of Omi/HtrA2 is positively correlated with aging markers. Overexpression of Omi/HtrA2 promoted myocardial aging.

Key words: Omi/HtrA2, myocardium, aging

CLC Number:

R542.2

Wei Xin, Liu Dan, Wu Ye, Lyu Kunyi, Shen Yan, Liu Huirong. Omi/HtrA2 overexpression is associated with myocardial aging[J]. Journal of Capital Medical University, 2021, 42(5): 783-789.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2021.05.014

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2021/V42/I5/783

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