Comparison between different methods to detect the subcellular localization of proteins

doi:10.3969/j.issn.1006-7795.2016.05.012

Journal of Capital Medical University ›› 2016, Vol. 37 ›› Issue (5): 616-620.doi: 10.3969/j.issn.1006-7795.2016.05.012

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Comparison between different methods to detect the subcellular localization of proteins

Ji Xiaofang¹, Li Liying², Chang Na²

1. Grade 2011 in Department of Basic Medicine, Capital Medical University, Beijing 100069, China;
2. Department of Cell Biology, School of Basic Medical Sciences, Municipal Laboratory for Liver Protection and Regulation of Regeneration, Capital Medical University, Beijing 100069, China

Received:2016-03-01 Online:2016-10-21 Published:2016-10-19
Supported by:
This study was supported by National Natural Science Foundation of China (31301154), Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges Under Beijing Municipality (IDHT20150502).

Abstract

Abstract: Objective To compare different methods for detecting the subcellular localization of proteins. Methods Using high content analysis to count the cytoplasmic or nuclear fluorescence intensity of proteins. Results Cytoplasmic or nuclear proteins were separated and detected by Western blotting. Conclusion The cytoplasmic translocation of protein was detected by these two different methods conclusion high content analysis is better than Western blotting for detecting the subcellular localization of proteins.

Key words: high content analysis, Western blotting, subcellular localization of protein

CLC Number:

Ji Xiaofang, Li Liying, Chang Na. Comparison between different methods to detect the subcellular localization of proteins[J]. Journal of Capital Medical University, 2016, 37(5): 616-620.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2016.05.012

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2016/V37/I5/616

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Comparison between different methods to detect the subcellular localization of proteins

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