Advanced glycation end products impair voltage-gated K+ channels in coronary vascular smooth muscle cells

doi:10.3969/j.issn.1006-7795.2016.06.002

Journal of Capital Medical University ›› 2016, Vol. 37 ›› Issue (6): 725-730.doi: 10.3969/j.issn.1006-7795.2016.06.002

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Advanced glycation end products impair voltage-gated K⁺ channels in coronary vascular smooth muscle cells

Su Wen¹, Li Hongwei¹, Chen Hui¹, Liu Huirong², Huang Haixia², Li Weiping¹

1. Department of Cardiology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China;
2. Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2016-10-03 Online:2016-12-21 Published:2016-12-16
Supported by:
This study was supported by National Natural Science Foundation of China(30971240), Natural Science Foundation of Beijing (7122053),High-level Technical Talents Foundation in Beijing Health System (2013-3-060).

Abstract

Abstract: Objective To investigate the role of advanced glycation end products (AGEs) in impairment of voltage-gated K⁺(K_v) channels in rat coronary vascular smooth muscle cells (VSMCs). Methods We isolated rat coronary VSMCs. The cells were incubated either in control medium, or medium with 100 μg/mL AGE-bovine serum albumin (AGE-BSA), or medium with 100 μg/mL AGE-BSA plus 100 μg/mL anti-receptor of AGEs immunoglobulin G (anti-RAGE IgG). Patch-clamp recording was used to assess the K_v currents. Western blotting and quantitative real-time PCR techniques were used to assess protein and mRNA expression of K_v 1.2 and K_v1.5. Results Incubation of VSMCs with AGE-BSA reduced K_v current density by 32.7% and decreased both protein and mRNA expression of K_v1.2 and K_v1.5 channels, whereas blocking AGE-BSA interacting with their receptors by preincubation with anti-RAGE IgG for 30 minutes prevented AGE-BSA-induced impairment of K_v channels.Conclusion AGEs impair K_v channels in coronary VSMCs by interacting with RAGE.

Key words: advanced glycation end products, voltage-gated K⁺ channels, coronary vascular smooth muscle cells, receptor of advanced glycation end products

CLC Number:

Su Wen, Li Hongwei, Chen Hui, Liu Huirong, Huang Haixia, Li Weiping. Advanced glycation end products impair voltage-gated K⁺ channels in coronary vascular smooth muscle cells[J]. Journal of Capital Medical University, 2016, 37(6): 725-730.

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Advanced glycation end products impair voltage-gated K⁺ channels in coronary vascular smooth muscle cells

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